Comprehensive identification of novel tumor markers by targeted shotgun proteomics

• Project/Area Number: 23650612
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Tumor diagnosis
• Research Institution: Jikei University School of Medicine (2012); Tokyo Medical and Dental University (2011)
• Principal Investigator: YOSHIDA Kiyotsugu 東京慈恵会医科大学, 医学部, 教授 (70345312)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: プロテオーム / 癌 / 腫瘍マーカー / プロテオミクス

Research Abstract

In an attempt to identify cancer-specific disease markers, we chose 2DICAL as a quantitative proteome analysis. By using 2DICAL, we found novel cancer-related genes.

Research Products

• [Journal Article] PDCD6, a novel p53-responsive gene, targets to the nucleus in the apoptotic response to DNA damage. (2012)
  • Author(s): Suzuki K, Dashzeveg N, Lu Z-G, Taira N, Miki Y, Yoshida K
  • Journal Title: Cancer Sci. Volume: 103 Pages: 1788-1894
• [Journal Article] DYRK2 priming phosphorylation of c-Jun and c-Myc modulates cell cycle progression in human cancer cells. (2012)
  • Author(s): Taira N, Mimoto R, Kurata M, Yamaguchi T, Kitagawa M, Miki Y, Yoshida K.
  • Journal Title: J Clin. Invest Volume: 122 Pages: 859-872
• [Journal Article] Post-translational modifications of p53 tumor suppressor: determinants of its functional targets. (2012)
  • Author(s): Taira N, Yoshida K
  • Journal Title: Histol. Histopathol. Volume: 27 Pages: 437-443
• [Journal Article] 抗腫瘍性キナーゼによる細胞周期制御とがんの進展 (2012)
  • Author(s): 平直江、吉田清嗣
  • Journal Title: 実験医学 Volume: 30 Pages: 1786-1789
• [Journal Article] DYRK2 priming phosphorylation of c-Jun and c-Myc modulates cell cycle progression in human cancer cells (2012)
  • Author(s): Taira N., et al
  • Journal Title: The Journal of Clinical Investigation Volume: 122 Issue: 3 Pages: 859-872
  • DOI: 10.1172/jci60818
  • Peer Reviewed
• [Journal Article] Programmed cell death 6, a novel p53-responsive gene, targets to the nucleus in the apoptotic response to DNA damage. (2012)
  • Author(s): Kazuho Suzuki
  • Journal Title: Cancer Sci. Volume: 103 Issue: 10 Pages: 1788-1794
  • DOI: 10.1111/j.1349-7006.2012.02362.x
  • Peer Reviewed
• [Journal Article] Identification of Evi-1 as a novel effector of PKCδin the apoptotic response to DNA damage. (2011)
  • Author(s): Hew HC, Liu H, Lu-Z-G, Kimura J, Miki Y, Yoshida K
  • Journal Title: Biochim. Biophys. Acta Volume: 1803 Pages: 285-294
• [Journal Article] PKCδregulates Mdm2 independently of p53 in the apoptotic response to DNA damage. (2011)
  • Author(s): Hew HC, Liu H, Miki Y, Yoshida K
  • Journal Title: Mol. Carcinog. Volume: 50 Pages: 719-731
• [Journal Article] 『アポトーシス誘導におけるPKC』 「Surgery Frontier」18:62-65 (2011) 8, Kimura J, Kudoh T, Miki Y, Yoshida K. Identification of dihydropyrimidinase-related protein 4 as a novel target of the p53 tumor suppressor in the apoptotic response to DNA damage. (2011)
  • Author(s): 吉田清嗣
  • Journal Title: Int. J. Cancer Volume: 128 Pages: 1524-1531
• [Journal Article] PKCdelta regulates Mdm2 independently of p53 in the apoptotic response to DNA damage. (2011)
  • Author(s): Hew, et al.
  • Journal Title: Mol. Carcinog. Volume: 50 Issue: 9 Pages: 719-731
  • DOI: 10.1002/mc.20748
• [Journal Article] Identification of Evi-1 as a novel effector of PKCdelta in the apoptotic response to DNA damage. (2011)
  • Author(s): Hew, et al.
  • Journal Title: Biochim. Biophys. Acta Volume: 1803 Pages: 285-294
• [Presentation] DYRK2 phosphorylation of c-Jun/c-Myc controls tumor progression by monitoring G1/S transition. (2013)
  • Author(s): Yoshida K
  • Organizer: 1st International Symposium on Protein Modifications in Pathogenic Dysregulation of Signaling.
  • Place of Presentation: Tokyo, Japan
• [Presentation] Identification of a novel mechanism for apoptosis and microRNA metabolism in response to genotoxic stress (2013)
  • Author(s): Taira N, Kimura J, Lu Z-G, Yamaguchi T, Yoshida K
  • Organizer: 9th Joint Conference of the American Association for Cancer Research and the Japanese Cancer Association
  • Place of Presentation: Maui, Hawaii
• [Presentation] The comprehensive study for the target genes of the Serine 46 phosphorylation of the p53. (2013)
  • Author(s): Dashzeveg N, Taira N, Miki Y, Yoshida K
  • Organizer: 9th Joint Conference of the American Association for Cancer Research and the Japanese Cancer Association
  • Place of Presentation: Maui, Hawaii
• [Presentation] DYRK2 phosphorylation of c-Jun/c-Myc controls tumor progression by monitoring G1/S transition. (2013)
  • Author(s): Kiyotsugu Yoshida
  • Organizer: 1st International Symposium on Protein Modifications in Pathogenic Dysregulation of Signaling
  • Place of Presentation: Tokyo, Japan
  • Invited
• [Presentation] 細胞死誘導と細胞周期制御による発癌抑制の分子機構 (2012)
  • Author(s): 吉田清嗣
  • Organizer: 第3次対がん10か年総合戦略・文科省がん支援活動 合同公開シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2012-01-31
• [Presentation] Role for DYRK2 in cell cycle control and tumor progression (2012)
  • Author(s): Yoshida K
  • Organizer: Siriaj Medical Journal 64: Suppl. 2, Nov-Dec. 61. (2012) MMC2012.
  • Place of Presentation: Bangkok, Thailand
• [Presentation] DYRK2 regulates breast cancer invasion via Snail/E-cadherin pathway (2012)
  • Author(s): Mimoto R, Imawari Y, Kamio M, Kato K, Nogi H, Toriumi Y, Takeyama H, Yoshida K,Uchida K
  • Organizer: The 2012 CTRC-AACR San Antonio Breast Cancer Symposium
  • Place of Presentation: San Antonio, TX
• [Presentation] Discovery of the pro-apoptotic genes induced by tumor suppressor p53 (2012)
  • Author(s): Dashzeveg N, Taira N, Miki Y, Yoshida K
  • Organizer: AACR Annual Meeting 2012
  • Place of Presentation: Chicago, IL
• [Presentation] DYRK2 regulates cancer invasiveness via Snail/E-cadherin pathway (2012)
  • Author(s): Mimoto R, Taira N, Miki Y, Yoshida K
  • Organizer: AACR Annual Meeting 2012
  • Place of Presentation: Chicago, IL
• [Presentation] DYRK2-mediated phosphorylation of c-Jun and c-Myc is requisite for proper control of the G1/S transition (2012)
  • Author(s): Taira N, Mimoto R, Miki Y, Yoshida K
  • Organizer: AACR Annual Meeting 2012
  • Place of Presentation: Chicago, IL
• [Presentation] Role for DYRK2 in cell cycle control and tumor progression. (2012)
  • Author(s): Kiyotsugu Yoshida
  • Organizer: MMC2012
  • Place of Presentation: Bangkok, Thailand
• [Presentation] アポトーシス誘導の制御機構 (2011)
  • Author(s): 吉田清嗣
  • Organizer: 第26回生体・生理工学シンポジウム/「バイオシグナルの統合と治療応用に関する研究会」第21回研究会
  • Place of Presentation: 草津、滋賀
  • Year and Date: 2011-09-21
• [Presentation] PKCδcontrols Evi-1 to transactivate PLZF in the apoptotic response to DNA damage (2011)
  • Author(s): Hew HC, Liu H, Lu-Z-G, Kimura J, Miki Y, Yoshida K
  • Organizer: 'Signal Transduction within the Nucleus', Gordon Research Conference.
  • Place of Presentation: Ventura, CA
• [Presentation] Amphiregulin, a novel target of p53, modulates microRNA metabolism in the nucleus. (2011)
  • Author(s): Taira N, Kimura J, Lu Z-G, Yamaguchi T, Higashiyama S, Ono M, Miki Y, Yoshida K
  • Organizer: 'Signal Transduction within the Nucleus', Gordon Research Conference.
  • Place of Presentation: Ventura, CA
• [Presentation] 細胞死誘導と細胞周期制御による発癌抑制の分子機構
  • Author(s): 吉田清嗣
  • Organizer: 第３次対がん１０か年総合戦略・文科省がん支援活動 合同公開シンポジウム
  • Place of Presentation: 東京
• [Book] Functional role for the c-Abl tyrosine kinase signalling in the DNA damage response. In: Kimura S and Shimizu S (eds), DNA repair: New Research. (2012)
  • Author(s): Yoshida K.
  • Publisher: Nova Science Publishers, Inc
• [Book] Role for PKCδon apoptosis in the DNA damage response. In: Clark C. Chen (ed) (2011)
  • Author(s): Yoshida K
  • Publisher: Selected Topics in DNA Repair.InTech
• [Book] Molecular function of the p53 tumor suppressor in the apoptotic response to DNA damage. in Chapter 12 of Tumor Suppressors. (2011)
  • Author(s): Yoshida K
  • Publisher: Nova Science Publishers, Inc
• [Remarks]
  • URL: http://www.jikei.ac.jp/academic/course/06_seikagaku.html