Development of non-invasive pancreatic cancer screening by detection of fecal miRNA

• Project/Area Number: 23650615
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Tumor diagnosis
• Research Institution: Okayama University
• Principal Investigator: NAGASAKA Takeshi 岡山大学, 岡山大学病院, 助教 (30452569)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 膵癌 / スクリーニング / miRNA / DNAメチル化 / 検診 / メチル化DNA

Research Abstract

We developed a new non-invasive screening strategy for pancreatic cancers by detection of fecal miRNA which might be expressed in organ-specific spectrum combined with our robust fecal DNA methylation analysis strategy.

Research Products

• [Journal Article] Serum mir-21 as a Diagnostic and Prognostic Biomarker in colorectal cancer (2013)
  • Author(s): 問山祐二、永坂岳司
  • Journal Title: JNCI Volume: (Epub ahead of print)
  • Peer Reviewed
• [Journal Article] MicroRNA-200c modulates epithelial-to-mesenchymal transition (EMT) in human colorectal cancer metastasis. (2013)
  • Author(s): Hur K, Toiyama Y, Takahashi M, Balaguer F, Nagasaka T, Koike J, Hemmi H, Koi M, Boland CR, Goel A
  • Journal Title: Gut Volume: 62 Issue: 9 Pages: 1315-26
  • DOI: 10.1136/gutjnl-2011-301846
  • Peer Reviewed
• [Journal Article] 便中メチル化CpG検出による大腸がんスクリーニング (2012)
  • Author(s): 永坂岳司、藤原俊義
  • Journal Title: 大腸癌フロンティア Volume: 5巻 Pages: 50-57
• [Journal Article] Characteristic patterns of altered DNA methylation predict emergence of human hepatocellular carcinoma (2012)
  • Author(s): 西田直生志、永坂岳司
  • Journal Title: Hepatology Volume: Vol.56 Pages: 994-1003
  • Peer Reviewed
• [Journal Article] MicroRNA-200c modulates epithelial-to-mesemchymal transition (EMT) in human colorectal cancer metastasis (2012)
  • Author(s): Keun Hur、永坂岳司
  • Journal Title: Gut
  • Peer Reviewed
• [Journal Article] 便中メチル化CpG検出による大腸がんスクリーニング (2012)
  • Author(s): 永坂岳司
  • Journal Title: 大腸癌フロンティア Volume: 5 Pages: 50-57
• [Journal Article] Characteristic patterns of altered DNA methylation predict emergence of human hepatocellular carcinoma (2012)
  • Author(s): Naoshi Nishida
  • Journal Title: Hepatology Volume: 56 Issue: 3 Pages: 994-1003
  • DOI: 10.1002/hep.25706
  • Peer Reviewed
• [Journal Article] Expansion of CpG methylation in the SFRP2 promoter resion during colorectal tumorigenesis (2011)
  • Author(s): 竹田正範、永坂岳司
  • Journal Title: Acta Medica Okayama Volume: Vol.65 Pages: 169-177
  • Peer Reviewed
• [Journal Article] De nova constitutional MLH1 epimutations confer early-onset colorectal cancer in two new sporadic Lynch syndrome cases, with derivation of the epimutation on the paternal allele in one (2011)
  • Author(s): Ajay Goel、永坂岳司
  • Journal Title: International of cancer Volume: Vol.128 Pages: 869-978
  • Peer Reviewed
• [Journal Article] Expansion of CpG Methylation in the SFRP2 Promoter Region during Colorectal Tumorigenesis (2011)
  • Author(s): Masanori Takeda, Takeshi Nagasaka
  • Journal Title: Acta Med. Okayama Volume: 65 Pages: 169-177
  • Peer Reviewed
• [Journal Article] De novo constitutional MLH1 epimutations confer early-onset colorectal cancer in two new sporadic Lynch syndrome cases, with derivation of the epimutation on the paternal allele in one (2011)
  • Author(s): Ajay Goel, Takeshi Nagasaka
  • Journal Title: international journal of cancer Volume: 128 Pages: 869-878
  • Peer Reviewed
• [Presentation] 便中メチル化検出による消化器癌スクリーニング (2013)
  • Author(s): 永坂岳司
  • Organizer: 第7回エピジェネティック研究会総会
  • Place of Presentation: 奈良市
  • Year and Date: 2013-05-31
• [Presentation] Analysis ofDNA methylation from exfoliated cancer cells in feces could screen colorectal tumors as well as other gastrointestinal cancers including pancreatic cancers (2013)
  • Author(s): 森川達也
  • Organizer: DDW2013
  • Place of Presentation: USA、オーランド
  • Year and Date: 2013-05-20
• [Presentation] Analysis of DNA methylation from exfoliated cancer cells in feces could screen colorectal tumors as well as other gastrointestinal cancers including pancreatic caners (2013)
  • Author(s): Tatsuya Morikawa
  • Organizer: 2013 DDW
  • Place of Presentation: アメリカ、オーランド
• [Presentation] 便中メチル化検出による消化器癌スクリーニング (2013)
  • Author(s): 永坂岳司
  • Organizer: 第7回日本エピジェネティック研究会総会
  • Place of Presentation: 奈良市
• [Presentation] 便中メチル化CpG検出による消化器癌スクリーニング (2012)
  • Author(s): 永坂岳司
  • Organizer: 第84回日本消化器内視鏡学会総会
  • Place of Presentation: 神戸市
  • Year and Date: 2012-10-11
• [Presentation] Fecal DNA Methylation Analysis to Detect Gastrointestinal Neoplasia (2012)
  • Author(s): 永坂岳司
  • Organizer: World Cancer Congress
  • Place of Presentation: カナダ、トロント
  • Year and Date: 2012-08-28
• [Presentation] FECAL DNA METHYLATION ANALYSIS TO DETECT GASTROINTESTINAL NEOPLASIA (2012)
  • Author(s): Takeshi nagasaka
  • Organizer: 2012 WORLD CANCER CONGRESS
  • Place of Presentation: カナダ、モントリオール
• [Presentation] BRAF変異癌-根治切除後の分ンし生物学的/臨床的特徴 (2011)
  • Author(s): 永坂岳司
  • Organizer: 第49回日本癌治療学会総会
  • Place of Presentation: 名古屋市
  • Year and Date: 2011-10-27
• [Presentation] 膵がんをターゲットにした非侵襲的スクリーニング技術の開発 (2011)
  • Author(s): 谷口信将
  • Organizer: 第111回日本外科学会総会
  • Place of Presentation: 紙上開催
• [Presentation] 膵がんをターゲットにした非侵襲的スクリーング技術の開発 / Power of non-invasive screening tool for pancreatic cancer - Does it leads to curative surgery? (2011)
  • Author(s): 谷口 信将, 永坂岳司
  • Organizer: 第111回日本外科学会定期学術集会シンポジウム
  • Place of Presentation: 紙上開催
• [Presentation] BRAF 変異大腸癌 -根治切除後の分子生物学的/臨床的特徴 (2011)
  • Author(s): 永坂岳司
  • Organizer: 第49回日本癌治療学会学術集会
  • Place of Presentation: 名古屋