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Development of an innovative method for evaluating DDS formulationpharmacokinetics by using multi-photon excitation microscopy

Research Project

Project/Area Number 23650621
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Clinical oncology
Research InstitutionTohoku University

Principal Investigator

OUCHI Noriaki  東北大学, 大学院・医学系研究科, 教授 (90203710)

Co-Investigator(Kenkyū-buntansha) GONDA Kosuke  東北大学, 大学院・医学系研究科, 准教授 (80375435)
AMARI Masakazu  東北大学, 大学院・医学系研究科, 准教授 (50400312)
TADA Hiroshi  東北大学, 大学院・医学系研究科, 助教 (50436127)
Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywordsドラッグデリバリー / 癌 / バイオテクノロジー / 薬学
Research Abstract

We prepared GFP-fused EB1-expressing human breast cancer cells. The growth rate of the microtubule plus-end was measured using the positions of EB1 assembly over time. Analysis of the dynamics of the EB1-GFP comet showed that the microtubule growth rates decreased when the cells were treated with anticancer drugs. We also measured the microtubule growth rates in tumor-bearing mice by using an original stereotaxic instrument. Thus, alteration in EB1-GFP dynamics is an effective indicator for the effects of anticancer drugs.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (28 results)

All 2012 2011

All Journal Article (14 results) (of which Peer Reviewed: 13 results) Presentation (8 results) Book (2 results) Patent(Industrial Property Rights) (4 results) (of which Overseas: 1 results)

  • [Journal Article] Multilayered, core/shell nanoprobes based on magnetic ferric oxide particles and quantum dots for multimodality imaging of breast cancer tumors.2012

    • Author(s)
      Qiang Ma, et al.
    • Journal Title

      Biomaterials

      Volume: 33 Issue: 33 Pages: 8486-8494

    • DOI

      10.1016/j.biomaterials.2012.07.051

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Development of a quantitative diagnostic method of estrogen receptor expression levels by immunohistochemistry using organic fluorescent material-assembled nanoparticles2012

    • Author(s)
      Gonda K, Miyashita M, Watanabe M, Takahashi Y, Goda H, Okada H, Nakano Y, Tada H, Amari M, Ohuchi N.
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 426(3) Issue: 3 Pages: 409-414

    • DOI

      10.1016/j.bbrc.2012.08.105

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Synthesis of high concentration colloid solution of silica-coated AgI nanoparticles2012

    • Author(s)
      Kobayashi Y, Minato M, Ihara K, Nakagawa T, Gonda K, Takeda M, Ohuchi N, Kasuya A.
    • Journal Title

      J Nanosci Nanotechnol

      Volume: 12(8) Issue: 8 Pages: 6741-6745

    • DOI

      10.1166/jnn.2012.4531

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Development of Silica-Coated Silver Iodide Nanoparticles and Their Biodistribution2012

    • Author(s)
      Sakurai Y, Tada H, Gonda K, Takeda M, Cong L, Amari M, Kobayashi Y, Watanabe M, Ishida T, Ohuchi N.
    • Journal Title

      The Tohoku Journal of Experimental Medicine

      Volume: 228 Issue: 4 Pages: 317-323

    • DOI

      10.1620/tjem.228.317

    • NAID

      130004460030

    • ISSN
      0040-8727, 1349-3329
    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Synthesis of Au-silica core-shell particles by sol-gel process2012

    • Author(s)
      Kobayashi Y, Inose H, Nakagawa T, Gonda K, Takeda M, Ohuhi N, Kasuya A.
    • Journal Title

      Surface Engineering

      Volume: 28(2) Issue: 2 Pages: 129-133

    • DOI

      10.1179/1743294411y.0000000069

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Fabrication andfluorescence properties of multilayered core-shell particles composed of quantum dot, gadolinium compound, and silica2012

    • Author(s)
      Kobayashi Y, Nozawa T, Nakagawa T, GondaK, Takeda M, Ohuchi N.
    • Journal Title

      J Mater Sci

      Volume: 47 Issue: 4 Pages: 1852-1859

    • DOI

      10.1007/s10853-011-5972-z

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] X-ray imaging ofnewly-developed gadolinium compound/silica core-shell particles2012

    • Author(s)
      Hikaru Morimoto, Marie Minato, Tomohiko Nakagawa, Masahide Sato, Yoshio Kobayashi, Kohsuke Gonda, Motohiro Takeda, Noriaki Ohuchi, Noboru Suzuki.
    • Journal Title

      Journal of Sol-Gel Science and Technology

      Volume: 59(3) Issue: 3 Pages: 650-657

    • DOI

      10.1007/s10971-011-2540-6

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Development of a quantitative diagnostic method of estrogen receptor expression levels by immunohistochemistry using organic fluorescent material-assembled nanoparticles.2012

    • Author(s)
      Gonda K
    • Journal Title

      Biochem Biophys Res Commun.

      Volume: 426 Pages: 409-414

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of silica-coated silver iodide nanoparticles and their biodistribution. Tohoku J Exp Med.2012

    • Author(s)
      Sakurai Y
    • Journal Title

      Tohoku J Exp Med.

      Volume: 228 Pages: 317-323

    • Related Report
      2012 Annual Research Report
  • [Journal Article] In vivo imaging of the molecular distribution of the VEGF receptor during angiogenesis in a mouse model of ischemia2011

    • Author(s)
      Hamada Y, Gonda K, Takeda M, Sato A, Watanabe M, Yambe T, Satomi S, Ohuchi N
    • Journal Title

      Blood

      Volume: 118 Issue: 13 Pages: e93-e100

    • DOI

      10.1182/blood-2010-12-322842

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Preparation of silica-coated AgI nanoparticles by an amine-free process and their X-ray imaging properties2011

    • Author(s)
      Ayame T, Kobayashi Y, Nakagawa T, Gonda K, Takeda M, Ohuchi N.
    • Journal Title

      Journal of the Ceramic Society of Japan

      Volume: 119 Issue: 1390 Pages: 397-401

    • DOI

      10.2109/jcersj2.119.397

    • NAID

      130000784598

    • ISSN
      1348-6535, 1882-0743
    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Control of shell thickness insilica-coating of Au nanoparticles and their X-ray imaging properties2011

    • Author(s)
      Kobayashi Y, Inose H, Nakagawa T, Gonda K, Takeda M, Ohuchi N, Kasuya A.
    • Journal Title

      J. Colloid Interface Sci

      Volume: 358(2) Issue: 2 Pages: 329-333

    • DOI

      10.1016/j.jcis.2011.01.058

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Single-particle tracking confirms that multivalent Tat-protein transduction domain induced Heparan-sulfate Proteoglycan (HSPG) cross-linkage activates Rac1 for internalization2011

    • Author(s)
      Imamura J, Suzuki Y, Gonda K, Roy CN, Gatabnaga H, Ohuchi N, Higuchi H
    • Journal Title

      J. Biol. Chem

      Volume: 286(12) Issue: 12 Pages: 10581-10592

    • DOI

      10.1074/jbc.m110.187450

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] In vivo imaging of the molecular distribution of the VEGF receptor during angiogenesis in a mouse model of ischemia2011

    • Author(s)
      Hamada Y, Gonda K, Takeda M, Sato A, Watanabe M, Yambe T, Satomi S, Ohuchi N
    • Journal Title

      Blood

      Volume: 118(13)

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] 抗がん剤の効果的ドラッグデリバリーへ向けた第一原理計算2012

    • Author(s)
      N.S.Venkataramanan,根城均,水関博志,川添良幸,権田幸祐,多田寛,大内憲明
    • Organizer
      ナノ学会第10回大会
    • Place of Presentation
      大阪
    • Related Report
      2012 Final Research Report
  • [Presentation] 抗がん剤の効果的ドラッグデリバリーへ向けた第一原理計算2012

    • Author(s)
      N.S.Venkataramanan
    • Organizer
      ナノ学会 第10回大会
    • Place of Presentation
      大阪
    • Related Report
      2012 Annual Research Report
  • [Presentation] がんの生体内分子メカニズム解明へ向けたin vivo分子イメージングの開発2012

    • Author(s)
      権田幸祐
    • Organizer
      ナノメディシン分子科学 第三回全体会議
    • Place of Presentation
      東京
    • Related Report
      2012 Annual Research Report
  • [Presentation] Nano-bio imaging of disease mechanisms with fluorescent nano-particles2012

    • Author(s)
      Gonda K
    • Organizer
      2012 Northeastern Asian Symposium 14th Biomaterial and Nano-bio Technology
    • Place of Presentation
      仙台
    • Related Report
      2012 Annual Research Report
  • [Presentation] パクリタキセル内包高分子ミセルの新たな抗腫瘍効果評価法2011

    • Author(s)
      濱中洋平,権田幸祐,武田元博,白石貢一,横山昌幸,大内憲明
    • Organizer
      第70回日本癌学会学術総会
    • Place of Presentation
      名古屋
    • Related Report
      2012 Final Research Report
  • [Presentation] DDS製剤の抗腫瘍効果イメージング2011

    • Author(s)
      濱中洋平,権田幸祐,武田元博,白石貢一,横山昌幸,大内憲明
    • Organizer
      第19回日本乳癌学会学術総会
    • Place of Presentation
      仙台
    • Related Report
      2012 Final Research Report 2011 Research-status Report
  • [Presentation] 血管新生における血管内皮増殖因子受容体分布の生体分子イメージング2011

    • Author(s)
      濱田庸,権田幸祐,武田元博,山家智之,佐藤成,大内憲明
    • Organizer
      ナノ学会第9回大会
    • Place of Presentation
      札幌
    • Related Report
      2011 Research-status Report
  • [Presentation] Development of a method to evaluate anti-cancer effect of polymeric micelles incorporating paclitaxel2011

    • Author(s)
      濱中洋平 ,権田幸祐,武田元博,白石貢一 ,横山昌幸,大内憲明
    • Organizer
      第70回日本癌学会学術総会
    • Place of Presentation
      名古屋
    • Related Report
      2011 Research-status Report
  • [Book] 生物物理"日本生物物理学会"京都2011

    • Author(s)
      権田幸祐,渡邉朋信,大内憲明,樋口秀男
    • Publisher
      がん転移の生体ナノイメージング
    • Related Report
      2012 Final Research Report
  • [Book] SURGERYFRONTIER"メディカルレビュー社"東京2011

    • Author(s)
      権田幸祐,樋口秀男,渡邉朋信,武田元博,大内憲明
    • Publisher
      ナノイメージングで探るがん転移の仕組み
    • Related Report
      2012 Final Research Report
  • [Patent(Industrial Property Rights)] 生体物質検出方法2012

    • Inventor(s)
      高梨健作,岡田尚大,中野寧,権田幸祐,大内憲明,渡邉みか
    • Industrial Property Rights Holder
      コニカミノルタエムジー株式会社,国立大学法人東北大学
    • Filing Date
      2012-09-04
    • Related Report
      2012 Final Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] 蛍光物質内包ナノ粒子およびこれを用いた生体物質の検出方法2011

    • Inventor(s)
      権田幸祐,大内憲明,渡邉みか,古澤直子,相宮拓司,中野寧
    • Industrial Property Rights Holder
      コニカミノルタエムジー株式会社,国立大学法人東北大学
    • Industrial Property Number
      2011-197340
    • Filing Date
      2011-09-09
    • Related Report
      2012 Final Research Report
  • [Patent(Industrial Property Rights)] 生体物質発現レベル評価システム2011

    • Inventor(s)
      権田幸祐,大内憲明,渡邉みか,相宮拓司,角森昭教,郷田秀樹,中野寧
    • Industrial Property Rights Holder
      コニカミノルタエムジー株式会社,国立大学法人東北大学
    • Industrial Property Number
      2011-197341
    • Filing Date
      2011-09-09
    • Related Report
      2012 Final Research Report
  • [Patent(Industrial Property Rights)] 生体物質検出方法2011

    • Inventor(s)
      権田幸祐,大内憲明,渡邉みか,高梨健作,岡田尚大,中野寧
    • Industrial Property Rights Holder
      コニカミノルタエムジー株式会社,国立大学法人東北大学
    • Industrial Property Number
      2011-197339
    • Filing Date
      2011-09-09
    • Related Report
      2012 Final Research Report

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Published: 2011-08-05   Modified: 2019-07-29  

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