Development of an innovative method for evaluating DDS formulationpharmacokinetics by using multi-photon excitation microscopy

• Project/Area Number: 23650621
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Clinical oncology
• Research Institution: Tohoku University
• Principal Investigator: OUCHI Noriaki 東北大学, 大学院・医学系研究科, 教授 (90203710)
• Co-Investigator(Kenkyū-buntansha): GONDA Kosuke 東北大学, 大学院・医学系研究科, 准教授 (80375435) ; AMARI Masakazu 東北大学, 大学院・医学系研究科, 准教授 (50400312) ; TADA Hiroshi 東北大学, 大学院・医学系研究科, 助教 (50436127)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: ドラッグデリバリー / 癌 / バイオテクノロジー / 薬学

Research Abstract

We prepared GFP-fused EB1-expressing human breast cancer cells. The growth rate of the microtubule plus-end was measured using the positions of EB1 assembly over time. Analysis of the dynamics of the EB1-GFP comet showed that the microtubule growth rates decreased when the cells were treated with anticancer drugs. We also measured the microtubule growth rates in tumor-bearing mice by using an original stereotaxic instrument. Thus, alteration in EB1-GFP dynamics is an effective indicator for the effects of anticancer drugs.

Research Products

• [Journal Article] Multilayered, core/shell nanoprobes based on magnetic ferric oxide particles and quantum dots for multimodality imaging of breast cancer tumors. (2012)
  • Author(s): Qiang Ma, et al.
  • Journal Title: Biomaterials Volume: 33 Issue: 33 Pages: 8486-8494
  • DOI: 10.1016/j.biomaterials.2012.07.051
  • Peer Reviewed
• [Journal Article] Development of a quantitative diagnostic method of estrogen receptor expression levels by immunohistochemistry using organic fluorescent material-assembled nanoparticles (2012)
  • Author(s): Gonda K, Miyashita M, Watanabe M, Takahashi Y, Goda H, Okada H, Nakano Y, Tada H, Amari M, Ohuchi N.
  • Journal Title: Biochem Biophys Res Commun Volume: 426(3) Issue: 3 Pages: 409-414
  • DOI: 10.1016/j.bbrc.2012.08.105
  • Peer Reviewed
• [Journal Article] Synthesis of high concentration colloid solution of silica-coated AgI nanoparticles (2012)
  • Author(s): Kobayashi Y, Minato M, Ihara K, Nakagawa T, Gonda K, Takeda M, Ohuchi N, Kasuya A.
  • Journal Title: J Nanosci Nanotechnol Volume: 12(8) Issue: 8 Pages: 6741-6745
  • DOI: 10.1166/jnn.2012.4531
  • Peer Reviewed
• [Journal Article] Development of Silica-Coated Silver Iodide Nanoparticles and Their Biodistribution (2012)
  • Author(s): Sakurai Y, Tada H, Gonda K, Takeda M, Cong L, Amari M, Kobayashi Y, Watanabe M, Ishida T, Ohuchi N.
  • Journal Title: The Tohoku Journal of Experimental Medicine Volume: 228 Issue: 4 Pages: 317-323
  • DOI: 10.1620/tjem.228.317
  • NAID: 130004460030
  • ISSN: 0040-8727, 1349-3329
  • Peer Reviewed
• [Journal Article] Synthesis of Au-silica core-shell particles by sol-gel process (2012)
  • Author(s): Kobayashi Y, Inose H, Nakagawa T, Gonda K, Takeda M, Ohuhi N, Kasuya A.
  • Journal Title: Surface Engineering Volume: 28(2) Issue: 2 Pages: 129-133
  • DOI: 10.1179/1743294411y.0000000069
  • Peer Reviewed
• [Journal Article] Fabrication andfluorescence properties of multilayered core-shell particles composed of quantum dot, gadolinium compound, and silica (2012)
  • Author(s): Kobayashi Y, Nozawa T, Nakagawa T, GondaK, Takeda M, Ohuchi N.
  • Journal Title: J Mater Sci Volume: 47 Issue: 4 Pages: 1852-1859
  • DOI: 10.1007/s10853-011-5972-z
  • Peer Reviewed
• [Journal Article] X-ray imaging ofnewly-developed gadolinium compound/silica core-shell particles (2012)
  • Author(s): Hikaru Morimoto, Marie Minato, Tomohiko Nakagawa, Masahide Sato, Yoshio Kobayashi, Kohsuke Gonda, Motohiro Takeda, Noriaki Ohuchi, Noboru Suzuki.
  • Journal Title: Journal of Sol-Gel Science and Technology Volume: 59(3) Issue: 3 Pages: 650-657
  • DOI: 10.1007/s10971-011-2540-6
  • Peer Reviewed
• [Journal Article] Development of a quantitative diagnostic method of estrogen receptor expression levels by immunohistochemistry using organic fluorescent material-assembled nanoparticles. (2012)
  • Author(s): Gonda K
  • Journal Title: Biochem Biophys Res Commun. Volume: 426 Pages: 409-414
  • Peer Reviewed
• [Journal Article] Development of silica-coated silver iodide nanoparticles and their biodistribution. Tohoku J Exp Med. (2012)
  • Author(s): Sakurai Y
  • Journal Title: Tohoku J Exp Med. Volume: 228 Pages: 317-323
• [Journal Article] In vivo imaging of the molecular distribution of the VEGF receptor during angiogenesis in a mouse model of ischemia (2011)
  • Author(s): Hamada Y, Gonda K, Takeda M, Sato A, Watanabe M, Yambe T, Satomi S, Ohuchi N
  • Journal Title: Blood Volume: 118 Issue: 13 Pages: e93-e100
  • DOI: 10.1182/blood-2010-12-322842
  • Peer Reviewed
• [Journal Article] Preparation of silica-coated AgI nanoparticles by an amine-free process and their X-ray imaging properties (2011)
  • Author(s): Ayame T, Kobayashi Y, Nakagawa T, Gonda K, Takeda M, Ohuchi N.
  • Journal Title: Journal of the Ceramic Society of Japan Volume: 119 Issue: 1390 Pages: 397-401
  • DOI: 10.2109/jcersj2.119.397
  • NAID: 130000784598
  • ISSN: 1348-6535, 1882-0743
  • Peer Reviewed
• [Journal Article] Control of shell thickness insilica-coating of Au nanoparticles and their X-ray imaging properties (2011)
  • Author(s): Kobayashi Y, Inose H, Nakagawa T, Gonda K, Takeda M, Ohuchi N, Kasuya A.
  • Journal Title: J. Colloid Interface Sci Volume: 358(2) Issue: 2 Pages: 329-333
  • DOI: 10.1016/j.jcis.2011.01.058
  • Peer Reviewed
• [Journal Article] Single-particle tracking confirms that multivalent Tat-protein transduction domain induced Heparan-sulfate Proteoglycan (HSPG) cross-linkage activates Rac1 for internalization (2011)
  • Author(s): Imamura J, Suzuki Y, Gonda K, Roy CN, Gatabnaga H, Ohuchi N, Higuchi H
  • Journal Title: J. Biol. Chem Volume: 286(12) Issue: 12 Pages: 10581-10592
  • DOI: 10.1074/jbc.m110.187450
  • Peer Reviewed
• [Journal Article] In vivo imaging of the molecular distribution of the VEGF receptor during angiogenesis in a mouse model of ischemia (2011)
  • Author(s): Hamada Y, Gonda K, Takeda M, Sato A, Watanabe M, Yambe T, Satomi S, Ohuchi N
  • Journal Title: Blood Volume: 118(13)
  • Peer Reviewed
• [Presentation] 抗がん剤の効果的ドラッグデリバリーへ向けた第一原理計算 (2012)
  • Author(s): N.S.Venkataramanan,根城均,水関博志,川添良幸,権田幸祐,多田寛,大内憲明
  • Organizer: ナノ学会第10回大会
  • Place of Presentation: 大阪
• [Presentation] 抗がん剤の効果的ドラッグデリバリーへ向けた第一原理計算 (2012)
  • Author(s): N.S.Venkataramanan
  • Organizer: ナノ学会 第10回大会
  • Place of Presentation: 大阪
• [Presentation] がんの生体内分子メカニズム解明へ向けたin vivo分子イメージングの開発 (2012)
  • Author(s): 権田幸祐
  • Organizer: ナノメディシン分子科学 第三回全体会議
  • Place of Presentation: 東京
• [Presentation] Nano-bio imaging of disease mechanisms with fluorescent nano-particles (2012)
  • Author(s): Gonda K
  • Organizer: 2012 Northeastern Asian Symposium 14th Biomaterial and Nano-bio Technology
  • Place of Presentation: 仙台
• [Presentation] パクリタキセル内包高分子ミセルの新たな抗腫瘍効果評価法 (2011)
  • Author(s): 濱中洋平,権田幸祐,武田元博,白石貢一,横山昌幸,大内憲明
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋
• [Presentation] DDS製剤の抗腫瘍効果イメージング (2011)
  • Author(s): 濱中洋平,権田幸祐,武田元博,白石貢一,横山昌幸,大内憲明
  • Organizer: 第19回日本乳癌学会学術総会
  • Place of Presentation: 仙台
• [Presentation] 血管新生における血管内皮増殖因子受容体分布の生体分子イメージング (2011)
  • Author(s): 濱田庸，権田幸祐，武田元博，山家智之，佐藤成，大内憲明
  • Organizer: ナノ学会第9回大会
  • Place of Presentation: 札幌
• [Presentation] Development of a method to evaluate anti-cancer effect of polymeric micelles incorporating paclitaxel (2011)
  • Author(s): 濱中洋平 ，権田幸祐，武田元博，白石貢一 ，横山昌幸，大内憲明
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋
• [Book] 生物物理"日本生物物理学会"京都 (2011)
  • Author(s): 権田幸祐,渡邉朋信,大内憲明,樋口秀男
  • Publisher: がん転移の生体ナノイメージング
• [Book] SURGERYFRONTIER"メディカルレビュー社"東京 (2011)
  • Author(s): 権田幸祐,樋口秀男,渡邉朋信,武田元博,大内憲明
  • Publisher: ナノイメージングで探るがん転移の仕組み
• [Patent(Industrial Property Rights)] 生体物質検出方法 (2012)
  • Inventor(s): 高梨健作,岡田尚大,中野寧,権田幸祐,大内憲明,渡邉みか
  • Industrial Property Rights Holder: コニカミノルタエムジー株式会社,国立大学法人東北大学
  • Filing Date: 2012-09-04
  • Overseas
• [Patent(Industrial Property Rights)] 蛍光物質内包ナノ粒子およびこれを用いた生体物質の検出方法 (2011)
  • Inventor(s): 権田幸祐,大内憲明,渡邉みか,古澤直子,相宮拓司,中野寧
  • Industrial Property Rights Holder: コニカミノルタエムジー株式会社,国立大学法人東北大学
  • Industrial Property Number: 2011-197340
  • Filing Date: 2011-09-09
• [Patent(Industrial Property Rights)] 生体物質発現レベル評価システム (2011)
  • Inventor(s): 権田幸祐,大内憲明,渡邉みか,相宮拓司,角森昭教,郷田秀樹,中野寧
  • Industrial Property Rights Holder: コニカミノルタエムジー株式会社,国立大学法人東北大学
  • Industrial Property Number: 2011-197341
  • Filing Date: 2011-09-09
• [Patent(Industrial Property Rights)] 生体物質検出方法 (2011)
  • Inventor(s): 権田幸祐,大内憲明,渡邉みか,高梨健作,岡田尚大,中野寧
  • Industrial Property Rights Holder: コニカミノルタエムジー株式会社,国立大学法人東北大学
  • Industrial Property Number: 2011-197339
  • Filing Date: 2011-09-09