Enhanced efficacy of adenovirus-mediated gene transfer by carbon nanotubes
| Project/Area Number |
23650623
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Clinical oncology
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| Research Institution | Shinshu University |
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Principal Investigator |
TAKEDA Teiji 信州大学, 医学部附属病院, 特任研究員 (90334896)
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| Co-Investigator(Kenkyū-buntansha) |
SAITO Naoto 信州大学, 医学部, 教授 (80283258)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | ドラッグデリバリー / アデノウイルス / カーボンナノチューブ |
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| Research Abstract |
Infection of adenovirus (Adv) has been widely used as a method of gene transfer into cells. We found that the Advs, pre-treated with carbon nanotubes (CNTs), obtained hundreds-fold higher trans-gene efficiency in cultured cells than the non-treated Advs. It was suggested that CNTs might form a complex with Advs, which efficiently delivered genes into cells through a different pathway from non-treated Advs. The enhanced effect for gene transfer into floating cells was also observed. Moreover CNT-treated Advs delivered object genes into tumor tissues of animal models with significantly higher efficiency than non-treated Advs.
These results indicate that our new system for gene transfer using CNT-treated Advs will contribute to many fields of molecular biology.
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Report
(4 results)
Research Products
(4 results)
