| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Research Abstract |
Infection of adenovirus (Adv) has been widely used as a method of gene transfer into cells. We found that the Advs, pre-treated with carbon nanotubes (CNTs), obtained hundreds-fold higher trans-gene efficiency in cultured cells than the non-treated Advs. It was suggested that CNTs might form a complex with Advs, which efficiently delivered genes into cells through a different pathway from non-treated Advs. The enhanced effect for gene transfer into floating cells was also observed. Moreover CNT-treated Advs delivered object genes into tumor tissues of animal models with significantly higher efficiency than non-treated Advs.
These results indicate that our new system for gene transfer using CNT-treated Advs will contribute to many fields of molecular biology.
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