| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Research Abstract |
We investigated silver nanocolloids (SNCs) effects on gene expression with DNA microarray analyses. 118 genes were up-regulated and 117 genes were down-regulated. Since severe morphological defects and damage of central nervous system were observed in SNCs-exposed medaka embryos, we chose five genes that were relevant to vertebrate embryogenesis and/or morphogenesis and well-studied in mammalian systems. Cathepsin L (ctsl), tropomyosin 1 alpha (tpm1), and retinol-binding protein (rbp) were significantly down-regulated in SNCs exposed embryos, while sarcoplasmic / endoplasmic reticulum calcium ATPase 1 (SERCA1, atp2a1) and homeobox B6b (hoxb6b) were both up-regulated. These genes were validated with with qRT-PCR. We performed gene knockdown techniques of RNAi. We observed the deformities in medaka embryos injected with siRNA of ctsl, tpm1 or rbp.We considered the three genes are target gene candidates of SNCs.
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