Molecular mechanism of radiation/NBS1-associated microcephaly

• Project/Area Number: 23651045
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Risk sciences of radiation/Chemicals
• Research Institution: Kyoto University
• Principal Investigator: KOMATSU Kenshi 京都大学, 放射線生物研究センター, 教授 (80124577)
• Project Period (FY): 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
• Keywords: 感受性 / 小頭症 / 放射線 / 大脳神経幹細胞 / 中心体 / アポトーシス / NBS1 / apical / basal

Research Abstract

The incidence of radiation microcephaly is 10-fold higher than that of radiation carcinogenesis. We showed her that radiation induces the apoptosis in basal neuro-progenitor cells, and develops radiation microcephaly. Simultaneously, apoptosis was observed in apical neuro-progenitor cells, which produce basal neuro-progenitor cells by cell proliferation through attachment of centrosomes to apical layers. These evidences suggest that proliferation is critical to develop radiation apososis, because defect in maintaining centrosomes induces the microcephaly in a patient with Nijmege breakage syndrome.

Research Products

• [Journal Article] 放射線によるDNA損傷の応答機構 (2012)
  • Author(s): 小松賢志
  • Journal Title: バイオクリニカ、北隆館 Volume: 27 Pages: 428-432
• [Journal Article] 放射線リスクと生物研究 (2012)
  • Author(s): 小松賢志
  • Journal Title: 京都大学人環フォーラム Volume: 30 Pages: 10-13
• [Journal Article] NBS1 recruits RAD18 via aRAD6-like domain and regulates Polη-dependent translesion DNA synthesis (2011)
  • Author(s): Yanagihara H, Kobayashi J, Tateishi S, Kato A, Matsuura S, Tauchi H, Yamada K, Takezawa J, Sugasawa K, Masutani C, Hanaoka F, Weemaes CM, Mori T, Zou L, Komatsu K.
  • Journal Title: Mol Cell Volume: 43 Issue: 5 Pages: 788-797
  • DOI: 10.1016/j.molcel.2011.07.026
  • Peer Reviewed
• [Journal Article] Genistein, isoflavonoids in soybeans, prevents the formation of excess radiation-induced centrosomes via p21up-regulation (2011)
  • Author(s): Shimada M, Kato A, Habu T, Komatsu K
  • Journal Title: Mutatation Research Volume: Volume 716 Issue: 1-2 Pages: 27-32
  • DOI: 10.1016/j.mrfmmm.2011.07.017
  • Peer Reviewed
• [Journal Article] Possible Role of WRN Protein in Cellular Response Induced by a Little DNA Damage (2011)
  • Author(s): Kobayashi J
  • Journal Title: Fusion Science and Technology Volume: 60 Pages: 1186-1189
  • Peer Reviewed
• [Journal Article] レントゲン以前の放射線修復遺伝子の役割は? (2011)
  • Author(s): 小松賢志
  • Journal Title: RIニュース Volume: 55 Pages: 2-3
• [Journal Article] RNF20によるヒストンH2Bのモノユビキチン化を介した新規DNA修復機構 (2011)
  • Author(s): 中村恭介
  • Journal Title: 放射線生物研究 Volume: 46(2) Pages: 191-196
• [Presentation] Novel Role of NBS1 in Ubiquitination-Mediated Response through RNF20 (2012)
  • Author(s): Junya Kobayashi, Kyosuke Nakamura, Hiromi Yanagihara, Akihiro Kato, Kenshi Komatsu
  • Organizer: 14th International Workshop on Ataxia-Telangiectasia(ATW2012)
  • Place of Presentation: New Delhi(India)
  • Year and Date: 2012-02-11
• [Presentation] Novel Role of NBS1 in Ubiquitination-Mediated Response through RNF20 (2012)
  • Author(s): Kobayashi J
  • Organizer: 14th International Workshop on Ataxia-Telangiectasia (ATW2012)
  • Place of Presentation: New Delhi (India)(招待講演)
  • Year and Date: 2012-02-11
• [Presentation] he roles of NBS1 in responses to radiation-and UV-induced DNA damage (2012)
  • Author(s): Kenshi Komatsu, Kyosuke Nakamura, Akihiro Kato, Junya Kobayashi, Hiromi Yanagihara, Douglas V. N. P. Oliveira, Mikio Shimada, Satoshi Tashiro, Shinya Matsuura, Toshio Mori, Hiroshi Tauchi
  • Organizer: The Sugahara Memorial International Symposium
  • Place of Presentation: Kyoto
  • Year and Date: 2012-01-25
• [Presentation] The roles of NBS1 in responses to radiation- and UV-induced DNA damage (2012)
  • Author(s): Komatsu K
  • Organizer: The Sugahara Memorial International Symposium
  • Place of Presentation: 京都大学(招待講演)
  • Year and Date: 2012-01-25
• [Presentation] Nucleolin participates in MDC1-related DNA damage response (2011)
  • Author(s): 小林純也、藤本浩子、林幾江、小松賢志
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-16
• [Presentation] Nucleolin participates in MDC1-related DNA damage response (2011)
  • Author(s): 小林純也
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-12-16
• [Presentation] H2B Ubiquitination-mediated chromatin remodeling in response to double-stranded DNA breaks in vertebrates (2011)
  • Author(s): Kenshi Komatsu, Kyosuke Nakamura, Akihiro Kato, Junya Kobayashi, Hiromi Yanagihara, Shuichi Sakamoto, Douglas V. N. P. Oliveira, Mikio Shimada, Satoshi Tashiro, Hiroshi Tauchi
  • Organizer: 日本分子生物学会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-12-15
• [Presentation] H2B ubiquitination-mediated chromatin remodeling in response to double-stranded DNA breaks in vertbrates (2011)
  • Author(s): Komatsu K
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(招待講演)
  • Year and Date: 2011-12-15
• [Presentation] CONTROL OF HOMOLOGOUS RECOMBINATION BY RNF20-DEPENDENT H2B UBIQUITINATION (2011)
  • Author(s): Kenshi Komatsu, Kyosuke Nakamura, Akihiro Kato, Junya Kobayashi
  • Organizer: 27th RBC-NIRS International Symposium
  • Place of Presentation: Kyoto
  • Year and Date: 2011-12-09
• [Presentation] CONTROL OF HOMOLOGUS RECOMBINATION BY RNF20-DEPENDENT H2B UBIQUITINATION (2011)
  • Author(s): Komatsu K
  • Organizer: 27th RBC-NIRS International Symposium
  • Place of Presentation: コープイン京都(招待講演)
  • Year and Date: 2011-12-09
• [Presentation] NBS1を中心とした放射線損傷応答の最近の進歩 (2011)
  • Author(s): 小松賢志
  • Organizer: 第2回国際放射線神経生物学会
  • Place of Presentation: 群馬大学
  • Year and Date: 2011-12-03
• [Presentation] NBS1を中心とした放射線損傷応答の最近の進歩 (2011)
  • Author(s): 小松賢志
  • Organizer: 第2回国際放射線神経生物学会
  • Place of Presentation: 群馬大学(招待講演)
  • Year and Date: 2011-12-03
• [Presentation] 酸化ストレスによるATMの活性制御 (2011)
  • Author(s): 小林純也、奥井理予、Martin Lavin、小松賢志
  • Organizer: 日本放射線影響学会第54回大会
  • Place of Presentation: 神戸
  • Year and Date: 2011-11-19
• [Presentation] 酸化ストレスによるATMの活性制御 (2011)
  • Author(s): 小林純也
  • Organizer: 日本放射線影響学会第54回大会
  • Place of Presentation: 神戸商工会議所会館
  • Year and Date: 2011-11-19
• [Presentation] グリオーマ幹細胞のDNA修復能 (2011)
  • Author(s): 高居邦友、宮澤浩人、小林純也、竹崎達也、秀拓一郎、平山亮一、近藤亨、小松賢志
  • Organizer: 日本放射線影響学会第54回大会
  • Place of Presentation: 神戸
  • Year and Date: 2011-11-18
• [Presentation] Facilitates Chromatin Transcription complex, FACT, on DNA repair (2011)
  • Author(s): Oliveira DV, Nakamura K, Kato A, Kobayashi J, Ikura T, Komatsu K
  • Organizer: 第54回日本放射線影響学会
  • Place of Presentation: 神戸
  • Year and Date: 2011-11-18
• [Presentation] MRN複合体とRAD51の相互作用 (2011)
  • Author(s): 加藤晃弘
  • Organizer: 日本放射線影響学会第54回大会
  • Place of Presentation: 神戸商工会議所会館
  • Year and Date: 2011-11-18
• [Presentation] グリオーマ幹細胞のDNA修復能 (2011)
  • Author(s): 高居邦友
  • Organizer: 日本放射線影響学会第54回大会
  • Place of Presentation: 神戸商工会議所会館
  • Year and Date: 2011-11-18
• [Presentation] Facilitates Chromatin Transcription complex, FACT, on DNA repair (2011)
  • Author(s): Oliveira D
  • Organizer: 日本分子生物学会
  • Place of Presentation: 神戸商工会議所会館
  • Year and Date: 2011-11-18
• [Presentation] Physical and functional interaction between MRN complex and RAD51 (2011)
  • Author(s): Akihiro Kato and Kenshi Komatsu
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 2011-10-05
• [Presentation] Physical and functional interaction between MRN couplex and RAD51 (2011)
  • Author(s): Kato A
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2011-10-05
• [Presentation] AT様疾患における酸化ストレスの増加はATM依存性DNA損傷応答に異常を引き起こす (2011)
  • Author(s): 小林純也、奥井理予、小松賢志
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋
  • Year and Date: 2011-10-03
• [Presentation] AT様疾患における酸化ストレスの増加はATM依存性DNA損傷応答に異常を引き起こす (2011)
  • Author(s): 小林純也
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2011-10-03
• [Presentation] FACT-dependent recruitment of RNF20 during DNA damage repair (2011)
  • Author(s): Douglas Oliveira, Ikura T, Nakamura K, Kato A, Kobayashi J, Komatsu K
  • Organizer: EMBO Workshop, Chromosome Structure, Damage & Repair
  • Place of Presentation: Cape Sounio, Greece
  • Year and Date: 2011-09-26
• [Presentation] FACT-dependent recruitment of RNF20 during DNA damage repair (2011)
  • Author(s): Oliveira D
  • Organizer: EMBO Workshop Chromosome Structure, Damage & Repair, September
  • Place of Presentation: Cape Sounio (Greece)
  • Year and Date: 2011-09-26
• [Presentation] Nucleolin participates in MDC1-related DNA damage response (2011)
  • Author(s): Junya Kobayashi, Hiroko Fujimoto, Kenshi Komatsu
  • Organizer: 14th International Congress of Radiation Research
  • Place of Presentation: Warsaw, Poland
  • Year and Date: 2011-08-30
• [Presentation] The link between MRN complex and RAD51 (2011)
  • Author(s): Akihiro Kato and Kenshi Komatsu
  • Organizer: 14th International Congress of Radiation Research Warsaw
  • Place of Presentation: Poland
  • Year and Date: 2011-08-30
• [Presentation] Nucleolin participates in MDC1-related DNA damage response (2011)
  • Author(s): Kobayashi J
  • Organizer: 14th International Congress of Radiation Research
  • Place of Presentation: Warsaw (Poland)
  • Year and Date: 2011-08-30
• [Presentation] The link between MRN complex and RAD51 (2011)
  • Author(s): Kato A
  • Organizer: 14th International Congress of Radiation Research
  • Place of Presentation: Warsaw (Poland)
  • Year and Date: 2011-08-30
• [Presentation] Ionizing radiation induced-microcephaly depends on dissociation of apical structure during embryonic development of the mouse cerebral cortex (2011)
  • Author(s): Mikio Shimada, J. N. Pulvers, F. Matsuzaki, K. Komatsu
  • Organizer: 14th International Congress of Radiation Research Warsaw
  • Place of Presentation: Poland
  • Year and Date: 2011-08-29
• [Presentation] Ionizing radiation induced-microcephaly depends on dissociation of apical structure during embryonic development of the mouse cerebral cortex (2011)
  • Author(s): Shimada M
  • Organizer: 14th International Congress of Radiation Research
  • Place of Presentation: Warsaw (Poland)
  • Year and Date: 2011-08-29
• [Presentation] DNA二重鎖切断におけるクロマチン・リモデリング因子とその制御機構 (2011)
  • Author(s): 小松賢志
  • Organizer: 環境エピゲノミクス研究会第5回定例会
  • Place of Presentation: 大阪府立大学
  • Year and Date: 2011-05-14
• [Presentation] DNA二重鎖切断におけるクロマチン・リモデリング因子とその制御機構 (2011)
  • Author(s): 小松賢志
  • Organizer: 環境エピゲノミクス研究会第5回定例会
  • Place of Presentation: 大阪府立大学(招待講演)
  • Year and Date: 2011-05-14
• [Presentation] MRN複合体とRAD51の相互作用
  • Author(s): 加藤晃弘、小松賢志
  • Organizer: 日本放射線影響学会第54回大会
  • Place of Presentation: 神戸
• [Book] DNA修復とゲノム不安定性 (2011)
  • Author(s): 小松賢志
  • Publisher: 羊土社
• [Book] DNA修復とゲノム不安定性 (2011)
  • Author(s): 小松賢志
  • Publisher: がん生物学イラストレイテッド、羊土社
• [Remarks]
  • URL: http://www.rbc.kyoto-u.ac.jp/index.html
• [Remarks]
  • URL: http://www.rbc.kyoto-u.ac.jp/index.html