Establishment of radiation-carcinogenesis system using culturedthyroid follicular cells

• Project/Area Number: 23651048
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Risk sciences of radiation/Chemicals
• Research Institution: Nagasaki University
• Principal Investigator: SUZUKI Keiji 長崎大学, 大学院・医歯薬学総合研究科, 准教授 (00196809)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 放射線 / がん / 甲状腺 / 発がん / 幹細胞

Research Abstract

We have isolated an immortalized cells derived from human thyroid follicles. These cells transcribe the PAX-8 gene but do not express any stem cell marker. After gamma-irradiation, cells were maintained at confluence to check focus formation. We observed dose-dependent foci formation, however, foci-derived cells lack transformed phenotypes, indicating that malignant conversion still requires additional genetic alterations

Research Products

• [Journal Article] Low-dose Radiation Exposure and Carcinogenesis (2012)
  • Author(s): Suzuki K, Yamashita S
  • Journal Title: Jpn J Clin Oncol Volume: 42 Pages: 563-568
  • NAID: 120006985780
  • Peer Reviewed
• [Journal Article] 発がんゲノム変異から見た低線量放射線リスクの考察 (2012)
  • Author(s): 鈴木啓司
  • Journal Title: 放射線生物研究 Volume: 47 Pages: 248-271
  • Peer Reviewed
• [Journal Article] Dedifferentiation of human primary thyrocytes into multilineage progenitor cells without gene introduction (2011)
  • Author(s): Suzuki K, Mitsutake N, Saenko V, Suzuki M, Matsuse M, Ohtsuru A, Kumagai A, Uga T, Yano H, Nagayama Y, Yamashita S
  • Journal Title: PLoS One Volume: 6
  • NAID: 120006985082
  • Peer Reviewed
• [Presentation] DNA損傷応答と放射線発がんメカニズム (2012)
  • Author(s): 鈴木啓司、山下俊一
  • Organizer: 日本薬学会フォーラム
  • Place of Presentation: 名古屋
• [Presentation] Molecular mechanism of radiation-induced thyroid cancer
  • Author(s): Keiji Suzuki
  • Organizer: Sugahara Memorial International Symposium
  • Place of Presentation: Kyoto
• [Book] 分裂死-mitotic catastrophe- (2012)
  • Author(s): 鈴木啓司
  • Publisher: 羊土社
• [Book] がん生物学 (2012)
  • Author(s): 鈴木啓司
  • Publisher: 羊土社
• [Remarks]
  • URL: http://www-sdc.med.nagasaki-u.ac.jp/drms/