Establishment of spatio-temporal analysis for low-dose-rateradiation-induced damage accumulation in tissue stem cells

• Project/Area Number: 23651051
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Risk sciences of radiation/Chemicals
• Research Institution: 一般財団法人電力中央研究所 (2012); Central Research Institute of Electric Power Industry (2011)
• Principal Investigator: OTSUKA Kensuke 一般財団法人電力中央研究所, 原子力技術研究所, 主任研究員 (50371703)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: 放射線 / 組織 / 細胞 / 癌 / 発生 / 分化 / 遺伝子 / 幹細胞 / 低線量 / 腸管 / 組織・細胞 / 発生・分化 / ターンオーバー / 障害蓄積性

Research Abstract

Radiation-induced turnover of tissue stem cells was evaluated by genetic lineagetracing of intestinal tissue stem cells (Lgr5+ stem cells). X-rays induced loss of Lgr5+stem cells and reduced Lgr5 gene expression in the colon but not in the duodenum. Lossof colonic Lgr5+ stem cells seemed to be compensated by de novo production of Lgr5+ stemcells. These findings are important evidences to understand radiation-inducedcarcinogenesis.

Research Products

• [Journal Article] Ionizing Radiation Leads to the Replacement and de novo Production of Colonic Lgr5+ Stem Cells (2013)
  • Author(s): Kensuke Otsuka
  • Journal Title: Radiation Research Volume: 179 Issue: 6 Pages: 637-646
  • DOI: 10.1667/rr3253.1
  • Peer Reviewed
• [Presentation] Evaluation of theradiation-induced turnover ofintestinal stem cells (2012)
  • Author(s): Kensuke Otsuka
  • Organizer: Annual Meetingof Radiation Research Society
  • Place of Presentation: Rio Mar Resort Hotel (アメリカ)
  • Year and Date: 2012-10-01
• [Presentation] Evaluation of theradiation-induced turnover ofintestinal stem cells (2012)
  • Author(s): 大塚健介
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: ロイトン札幌(北海道)
  • Year and Date: 2012-09-21
• [Presentation] Evaluation of theradiation-induced turnover ofintestinal stem cells by Lgr5 lineagetagging system (2011)
  • Author(s): 大塚健介
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場(愛知)
  • Year and Date: 2011-10-05
• [Presentation] Evaluation of radiation-induced turnover of intestinal stem cells by Lgr5-lineage tagging system (2011)
  • Author(s): 大塚 健介
  • Organizer: 日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場（愛知県）
• [Presentation] 腸管組織の幹細胞ターンオーバー (2011)
  • Author(s): 大塚 健介
  • Organizer: 日本放射線影響学会
  • Place of Presentation: 神戸商工会議所会館（兵庫県）
• [Presentation] Evaluation of the radiation-induced turnover of intestinal stem cells
  • Author(s): 大塚 健介
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: ロイトン札幌（北海道）
• [Presentation] Evaluation of the radiation-induced turnover of intestinal stem cells
  • Author(s): Kensuke Otsuka
  • Organizer: Annual Meeting of Radiation Research Society
  • Place of Presentation: Rio Mar Resort Hotel（アメリカ）