Mechanizme analysis of accumulation of PFOS in blood
| Project/Area Number |
23651053
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Risk sciences of radiation/Chemicals
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| Research Institution | Kyushu University |
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Principal Investigator |
OSHIMA Yuji 九州大学, 農学研究院, 教授 (70176874)
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| Co-Investigator(Kenkyū-buntansha) |
島崎 洋平 九州大学, 農学研究院 (40363329)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | PFOS 結合タンパク質 / 魚類 / 血液 / PFOS / 結合タンパク質 |
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| Research Abstract |
Perfluorooctane sulfonate (PFOS) was known to accumulate in blood of fish, bird and mammals. PFOS might bind with unknown substance in blood, however the mechanism is unclear. Thus, we performed to purify a PFOS binding substance from fish blood and confirmed its affinity to PFOS. As results, PFOS binding protein was successfully purified from the plasma of PFOS administered-puffer fish (Takifugu rubripes) from the 65-70% ammonium sulfate precipitation. N-terminal analysis strongly suggested that PFOS-binding protein was an apolipoprotein A-I in T. rubripes. In accorded this, PFOS was confirmed to bind with the 65-70% ammonium sulfate fraction prepared from non-PFOS treated T. rubripes. Those results indicated that PFOS was bind to apolipoprotein A-I. Thus, this may suggest disorder of physiological function because apolipoprotein A-I may play an important role in lipid metabolism in fish.
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Report
(3 results)
Research Products
(1 results)
