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Chemical Developmental Toxicity Test Using A New Material Introducing Technique for Medaka Egg

Research Project

Project/Area Number 23651057
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Risk sciences of radiation/Chemicals
Research InstitutionAriake National College of Technology

Principal Investigator

TOMINAGA Nobuaki  有明工業高等専門学校, 物質工学科, 教授 (30227631)

Co-Investigator(Kenkyū-buntansha) KONO Susumu  有明工業高等専門学校, 電気工学科, 教授 (30270375)
有薗 幸司  熊本県立大学, 環境共生学部, 教授 (70128148)
Co-Investigator(Renkei-kenkyūsha) YAMAGUCHI Akemi  有明工業高等専門学校, 技術支援センター, 専門職員 (90399262)
Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords発生影響評価法 / パルスパワー / メダカ / DNAマイクロアレイ / タンパク合成阻害剤 / トランスクリプトーム / フェノーム / 発生影響 / マイクロアレイ
Research Abstract

We developed a novel method of chemical developmental toxicity assay using pulsed power electroporation chemical transfer technique for fertilized medaka egg. We could find the best pulsed power treatment condition which introduced chemicals efficiently and did not affected embryogenesis and gene expression. Then we measured uptake of bisphenol A under this condition by GC-MS. Bisphenol A concentration in egg extract was higher than immersed solution. According to DNA microarray data, we found the specific gene expression profile by uptake of bisphenol A.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (17 results)

All 2013 2012 2011 Other

All Journal Article (4 results) (of which Peer Reviewed: 2 results) Presentation (12 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Development of pulsed power supply for materials incorporation into medaka eggs2012

    • Author(s)
      Kubo T, Yamaguchi A, Tominaga N, Kono S
    • Journal Title

      International Symposium on Technology for Sustainerbility(ISTS2012)

      Volume: 1 Pages: 297-300

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A Study of Material Incorporation for Medaka (Oryzias latipes) Eggs by Various Voltage Pulses2011

    • Author(s)
      Susumu Kono et.al.
    • Journal Title

      Proc. of the 18th IEEE Int. Pulsed Power Conf.

      Volume: in press

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] A Study of Material Incorporation for Medaka (Oryzias latipes) Eggs by Various Voltage Pulses

    • Author(s)
      Susumu Kono, Akemi Yamaguchi, Takashi Tanabe, Nobuaki Tominaga, Hidenori Akiyama
    • Journal Title

      Proc. of the 18th IEEE Int. Pulsed Power Conf.

    • Related Report
      2012 Final Research Report
  • [Journal Article] Development of pulsed power suppl y for materials incorporation into medaka eggs

    • Author(s)
      Kubo T, Yamaguchi A, Tominaga N, Kono S
    • Journal Title

      International Symposium on Technology for Sustainability (ISTS2012)

      Pages: 297-300

    • Related Report
      2012 Final Research Report
  • [Presentation] 化学物質暴露メダカにおけるエストロジェン応答遺伝子の発現変動2012

    • Author(s)
      冨永伸明
    • Organizer
      リスクサイエンス研究フォーラム2012(招待講演)
    • Place of Presentation
      福岡大学(福岡市)
    • Related Report
      2011 Research-status Report
  • [Presentation] 高電界パルスによるメダカ卵への物質導入;分子量と導入効率についての検討2011

    • Author(s)
      山口明美 他
    • Organizer
      第64回 電気関係学会九州支部連合大会
    • Place of Presentation
      佐賀大学(佐賀市)
    • Related Report
      2011 Research-status Report
  • [Presentation] 極性の異なる2連続パルスによるメダカ卵への影響2011

    • Author(s)
      今村泰隆 他
    • Organizer
      第64回 電気関係学会九州支部連合大会
    • Place of Presentation
      佐賀大学(佐賀市)
    • Related Report
      2011 Research-status Report
  • [Presentation] 高電界パルスを用いた化学物質の初期発生影響評価法の開発2011

    • Author(s)
      山口明美 他
    • Organizer
      第14回日本環境ホルモン学会研究発表会
    • Place of Presentation
      東京大学(東京)
    • Related Report
      2011 Research-status Report
  • [Presentation] 異なる作用機構をもつタンパク合成阻害剤がメダカ受精卵に与える影響2011

    • Author(s)
      山口明美 他
    • Organizer
      フォーラム2011衛生薬学・環境トキシコロジー
    • Place of Presentation
      金沢市
    • Related Report
      2011 Research-status Report
  • [Presentation] Improvement of pulsed power system for incorporating materials into medaka eggs

    • Author(s)
      Kono S, Yamaguchi A, Kubo T, Imamura Y, Tominaga N
    • Organizer
      9th International Bioelectronics Symposium
    • Related Report
      2012 Final Research Report
  • [Presentation] 水中放電による衝撃波が生体に及ぼす影響に関する基礎研究

    • Author(s)
      岩屋 健人,河野 晋,山口 明美,冨永 伸明,小田 達也
    • Organizer
      電気学会九州支部平成24年度(第3回)高専卒業研究発表会
    • Related Report
      2012 Final Research Report
  • [Presentation] 高電界パルスによるメダカ卵への物質導入;分子量と導入効率についての検討

    • Author(s)
      山口明美,今村泰隆,河野晋,冨永伸明
    • Organizer
      第64回電気関係学会九州支部連合大会
    • Related Report
      2012 Final Research Report
  • [Presentation] 極性の異なる2連続パルスによるメダカ卵への影響

    • Author(s)
      今村泰隆,山口明美,河野晋,冨永伸明
    • Organizer
      第64回電気関係学会九州支部連合大会
    • Related Report
      2012 Final Research Report
  • [Presentation] 化学物質暴露メダカにおけるエストロジェン応答遺伝子の発現変動

    • Author(s)
      冨永伸明
    • Organizer
      リスクサイエンス研究フォーラム2012
    • Related Report
      2012 Final Research Report
  • [Presentation] 水中放電による衝撃波が生体に及ぼす影響に関する基礎研究

    • Author(s)
      岩屋健人,河野晋,山口明美,冨永伸明,小田達也
    • Organizer
      電気学会九州支部平成24年度高専卒業研究発表会
    • Place of Presentation
      佐世保
    • Related Report
      2012 Annual Research Report
  • [Presentation] Improvement of pulsed power system for incorporating materials into medaka eggs

    • Author(s)
      Kono S, Yamaguchi A, Kubo T, Imamura Y, Tominaga N
    • Organizer
      9th International Bioelectronics Symposium
    • Place of Presentation
      Kumamoto
    • Related Report
      2012 Annual Research Report
  • [Patent(Industrial Property Rights)] 物質導入方法およびその装置2013

    • Inventor(s)
      河野晋,冨永伸明,山口明美
    • Industrial Property Rights Holder
      独立行政法人国立高等専門学校機構種類
    • Industrial Property Number
      2013-120148
    • Filing Date
      2013-06-06
    • Related Report
      2012 Final Research Report

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Published: 2011-08-05   Modified: 2019-07-29  

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