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Creation of the biological flocculant and inorganic capture protein for environmental purification

Research Project

Project/Area Number 23651078
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Environmental technology/Environmental materials
Research InstitutionSetsunan University

Principal Investigator

HIROSHI Oyama  摂南大学, 理工学部, 教授 (50221700)

Co-Investigator(Kenkyū-buntansha) SUGIMURA Yukio  衣笠会繊維研究所, 理事 (20273542)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Keywords濁水浄化 / タンパク質 / モリンガ / 凝集 / 組換え / 種子 / 金属
Research Abstract

We determined the nucleotide sequence of the structural gene of the protein having an excellent flocculation capacity contained in the seed of a tropical tree, the Moringa oleifera. The protein coagulates soil particle and purifies turbid water. Compared with its deduced amino acid sequence and protein sequences of S and L subunits, we predicted that the Moringa flocculation protein is composed of signal peptide, propeptide, S subunit and linker peptide. We succeeded in the gene expression of S subunit and of the S and L subunits including linker peptide. Based on this result, the construction of the purification system of turbid water and the development of metal capture protein may be enabled.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (13 results)

All 2013 2012 2011 Other

All Journal Article (5 results) (of which Peer Reviewed: 4 results) Presentation (5 results) Book (2 results) Remarks (1 results)

  • [Journal Article] Subcellular compartmentation of strontium and zinc in mulberry idioblasts in relation to phytoremediation potential2013

    • Author(s)
      Hisato Katayama, Naoto Banba, Yukio Sugimura, Makoto Tatsumi, Shin-ichi Kusakari, Hiroshi Oyama, Atsushi Nakahira
    • Journal Title

      Environmental and Experimental Botany

      Volume: 85 Pages: 30-35

    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Journal Article] Visualization of mitochondria in living cells with a genetically encoded yellow fluorescent protein originating from a yellow-emitting luminous bacterium2013

    • Author(s)
      Hajime Karatani, Yuki Namikawa, Naomi Mori, Yukihiro Nishikawa, Saki Imai, Yutaka Ihara, Aya Kinoshita, Kengo Kitadokoro, Hiroshi Oyama
    • Journal Title

      Photochemical & Photobiological Sciences

      Volume: 12 Pages: 944-956

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Covalent Chromatography for Chymotrypsin-like Proteases Using a Diphenyl 1-Amino-2-phenylethylphosphonate Derivative2013

    • Author(s)
      Shin Ono, Junya Murai, Seigo Furuta, Kazuya Doike, Fumie Manzaki, Toshiaki Yoshimura, Hirofumi Kuroda, Masahito Umezaki, Hiroshi Oyama
    • Journal Title

      Journal of Biological Macromolecules

      Volume: 13 Pages: 78-85

    • NAID

      130007492675

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Altered gene expression profiles associated with enhanced skin inflammation induced by 12-O-tetradecanoylphorbol-13-acetate in streptozotocin-diabetic mice2013

    • Author(s)
      Yoshinori Iba, Koushi Watanabeb, Kiyokazu Ozaki, Osamu Aozasa, Keisuke Ishizawa, Tetsuro Matsuura, Hiroshi Oyama, Tohru Masukawa
    • Journal Title

      International Immunopharmacology

      Volume: 15 Issue: 3 Pages: 614-619

    • DOI

      10.1016/j.intimp.2013.01.007

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Assessment of Substrate Inhibition of Bacterial Oligopeptidase B2012

    • Author(s)
      Malik Suliman Mohamed Mustafa, Yoshitaka Nakajima, Hiroshi Oyama, Nobuhisa Iwata, Kiyoshi Ito
    • Journal Title

      Biological and Pharmaceutical Bulletin

      Volume: 35 Issue: 11 Pages: 2010-2016

    • DOI

      10.1248/bpb.b12-00544

    • NAID

      130001872267

    • ISSN
      0918-6158, 1347-5215
    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] クマモリシンによる魚鱗の分解とその分解産物のDPP-IV阻害活性2012

    • Author(s)
      尾山 廣、井上 直樹、小泉 聖子、杉原 富人、居場 嘉教、芳本 忠
    • Organizer
      日本農芸化学会
    • Place of Presentation
      京都女子大学(京都市)
    • Related Report
      2011 Research-status Report
  • [Presentation] 耐熱性セリン‐カルボキシルプロテアーゼ、kumamolisinsの構造と機能2011

    • Author(s)
      尾山廣、伊東昌章、中山亨
    • Organizer
      日本生化学会(シンポジウム)
    • Place of Presentation
      国立京都国際会館(京都市)
    • Related Report
      2011 Research-status Report
  • [Presentation] カイコ幼虫後部絹糸腺由来無細胞タンパク質合成系における翻訳促進配列の機能解析

    • Author(s)
      佐事 武、尾山 廣、伊東 昌章
    • Organizer
      日本農芸化学会2014年度大会
    • Place of Presentation
      明治大学生田キャンパス
    • Related Report
      2013 Annual Research Report
  • [Presentation] 細菌由来のオリゴペプチダーゼBの基質阻害

    • Author(s)
      Malik Suliman Mohamed Mustafa、中嶋義隆、尾山廣、芳本忠、小山裕也、大河原晋、岩田修永、伊藤潔
    • Organizer
      第85回日本生化学会大会
    • Place of Presentation
      福岡国際会議場(福岡市)
    • Related Report
      2012 Research-status Report
  • [Presentation] 耐熱性セリン‐カルボキシルプロテアーゼ、クマモリシンの活性化機構の解析

    • Author(s)
      塩川史弥、伊東昌章、尾山廣
    • Organizer
      日本農芸化学会2013年度大会
    • Place of Presentation
      東北大学(仙台市)
    • Related Report
      2012 Research-status Report
  • [Book] Peptide Science 2012 (Length-Sorting Single-Walled Carbon Nanotubes by Bovine a-Casein on a Gel Filtration Chromatography)2012

    • Author(s)
      Shin Ono, Kazuma Gomi, Risa Yamakoshi, Hiroshi Oyama, Hougen Yamamoto, Yasushi Maeda
    • Publisher
      財団法人蛋白質研究奨励会
    • Related Report
      2012 Research-status Report
  • [Book] Peptide Science 2011 (Ed. by K. Sakaguchi)2012

    • Author(s)
      Junya Murai, Yuki Sakai, Hiroshi Oyama, and Shin Ono
    • Publisher
      Affinity Purification of alpha-Amylase Using 0.19 alpha-Amylase Inhibitor from Wheat Kernel.
    • Related Report
      2011 Research-status Report
  • [Remarks] 生体分子機能学研究室ホームページ

    • URL

      http://www.setsunan.ac.jp/~bio/labo/oyama.html

    • Related Report
      2013 Annual Research Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

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