Construction of the homing cells harboring sensitive fluorescence probes for detection of environmental pollutants

• Project/Area Number: 23651086
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Environmental technology/Environmental materials
• Research Institution: 大学共同利用機関法人自然科学研究機構(岡崎共通研究施設) (2012); National Institutes of Natural Sciences Okazaki Research Facilities (2011)
• Principal Investigator: AONO Shigetoshi 大学共同利用機関法人自然科学研究機構(岡崎共通研究施設), 岡崎統合バイオサイエンスセンター, 教授 (60183729)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 環境技術 / 生物物理 / 蛋白質 / 生体機能利用 / 重金属センサー / 蛍光プローブ / 蛍光プローブ分子

Research Abstract

In this work, the construction of FRET-type fluorescence probes using HRM（heme regulatory motif）or transition metal ions binding protein SmtA was tried.Structural and functional characterization of the transition metal ion-containing chemotaxis signal transducer protein was carried out to obtain basic information for the construction of homing cells that can swarm spontaneously toward transition metal ions.

Research Products

• [Journal Article] Structural Basis for Oxygen Sensing and Signal Transduction of the Heme-Based Sensor Protein Aer2 from Pseudomonas aeruginosa (2012)
  • Author(s): H. Sawai, H. Sugimoto, Y. Shiro, H. Ishikawa, Y. Mizutani, S. Aono
  • Journal Title: Chem. Commun Volume: 48 Issue: 52 Pages: 6523-6525
  • DOI: 10.1039/c2cc32549g
  • Peer Reviewed
• [Presentation] Listeria monocytogenes のヘム取込み系におけるヘム結合タンパク質HupD の性質 (2013)
  • Author(s): 岡本泰典
  • Organizer: 日本化学会第93春季年会
  • Place of Presentation: 立命館大学(滋賀県)
• [Presentation] Listeria monocytogenes のヘム取込み系におけるヘム結合タンパク質HupDの性質 (2013)
  • Author(s): 岡本泰典
  • Organizer: 日本化学会第９３春季年会
  • Place of Presentation: 立命館大学（滋賀県）
• [Presentation] Structural characterization of Aer2 that adopts a heme-containing PAS domain as a sensor for aerotaxis control (2011)
  • Author(s): H. Sawai
  • Organizer: 15th International Conference on Biological Inorganic Chemistry
  • Place of Presentation: Vancouver (Canada)
• [Presentation] ヘムをシグナル分子とする生体機能制御の分子機構
  • Author(s): 青野重利
  • Organizer: 第１０８回触媒討論会(招待講演)
  • Place of Presentation: 北見工業大学（北海道）
• [Presentation] ヘムセンサー機能を有する転写調節因子の構造と機能
  • Author(s): 青野重利
  • Organizer: 第６１回錯体化学討論会
  • Place of Presentation: 岡山理科大学（岡山県）
• [Presentation] Biological Sensing Systems with Heme
  • Author(s): 青野重利
  • Organizer: ICBIC 15(招待講演)
  • Place of Presentation: Vancouver (Canand)