Development of a new strand-invader with artificial nucleotides
| Project/Area Number |
23651128
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Nanomaterials/Nanobioscience
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| Research Institution | Nagoya University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | DNA / ストランドインベージョン / インターカレーター / PNA / D-Threoninol / ストランドインベーダー / トレオニノール / Threoninol / 色素 |
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| Research Abstract |
In this project, we aimed at developing a new strand-invader by combining unmodified peptide nucleic acid (PNA) and modified DNA (TN -DNA) involving new base-surrogate=Threoninol-Nucleotide (TN) that tethers a functional molecule such as intercalator on D-threoninol. As a possible strand-invader, the duplex stability should be in the following order; DNA/PNA, TN-DNA/DNA > DNA/DNA > TN-DNA/PNA. First, we searched a functional molecule and optimum way of introducing TN into DNA that satisfies above requisites. We found that planar molecule (intercalator) with electron-withdrawing group could significantly stabilize the DNA duplex. Furthermore, introduction of TN at every two nucleotides into DNA remarkably facilitated the duplex formation with DNA and retarded hybridization with PNA. On the basis of these results, we combined unmodified PNA and TN-DNA involving anthraquinone as a strand-invader, which was successfully invaded into the target DNA duplex.
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Report
(3 results)
Research Products
(13 results)
