A Genome-wide screening of circadian transcripts using a novel poly(A) determination method

Research Project

Project/Area Number	23651184
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Multi-year Fund
Research Field	Genome biology
Research Institution	Kanazawa University
Principal Investigator	TEI Hajime 金沢大学, 自然システム学系, 教授 (00242115)
Project Period (FY)	2011
Project Status	Completed (Fiscal Year 2011)
Budget Amount *help	¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000) Fiscal Year 2011: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Keywords	概日リズム / 時計遺伝子 / 視交叉上核 / Periodl / poly(A) / 翻訳制御
Research Abstract	We have developed a novel method to determine the length of poly(A) in eukaryotic mRNA, and designated as PACHINCO(Poly(A) Capture by Hairpin Chimeric Oligonucleotide)-RT-PCR. In this year, we optimized the application of the method to an automated microchip electrophoresis system(MultiNA, SHIMADZU), including the development of the distribution analysis algorithm of a variety of poly(A) length.