| Project/Area Number |
23651185
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Genome biology
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| Research Institution | Waseda University |
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Principal Investigator |
OHYAMA Takashi 早稲田大学, 教育・総合科学学術院, 教授 (60268513)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 減数分裂期組換え / 高頻度組換え部位 / DSBホットスポット / 相同組換え / 出芽酵母ゲノム / 分裂酵母ゲノム / マウスゲノム / 二本鎖DNA崩壊エネルギー / 減数分裂 / 組換えホットスポット / DSB / 遺伝情報 / 出芽酵母 / クロマチン / DNA物性 |
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| Research Abstract |
We explored characteristics of the physical properties and conformations of DSB (double-strand break) hotspots on the genomes of yeasts (Saccharomyces cerevisiae and Schizosaccharomyces pombe) and mouse (Mus musculus). When averaged, DSB hotspots of each organism clearly showed a distinctive mechanical property compared to the surrounding regions. However, very interestingly, the properties themselves were opposite between the yeasts and mouse: i.e., DSB hotspots are very rigid in the former while very flexible in the latter. Another physical property to be noted was that DSB hotspots of all the three organisms show distinctively high duplex disruption energy compared to the surrounding regions.
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