Development of proteomics technology using next generation sequencer
Project/Area Number |
23651190
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Genome biology
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Research Institution | The University of Tokyo |
Principal Investigator |
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Project Period (FY) |
2011 – 2012
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Project Status |
Completed (Fiscal Year 2013)
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Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
Keywords | プロテオーム / 次世代シーケンサ / インタラクトーム / IVV / プロテオソーム / 次世代シーケンサー |
Research Abstract |
In vitro virus (IVV) technology, a variation of mRNA display, is suitable for extensive protein-protein interaction analysis. Previous IVV method requires bait protein preparation which become the rate-limiting step. This work aimed to develop IVV method which is not need bait molecule purification. Chimeric DNA whose ends are corresponding to interacting pair can prepare by cross-linking of IVV molecule complex and following emulsion PCR, and next generation sequencing of the DNA can detect interacting pair data without bait preparation step. This novel method serves as the foundation of simple and comprehensive protein-protein interaction analysis.
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Report
(4 results)
Research Products
(62 results)
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[Journal Article] Mitochondria-nucleus shuttling FK506-binding protein 51 interacts with TRAF proteins and facilitates the RIG-I-like receptor-mediated expression of type I IFN.2014
Author(s)
Akiyama, T., Shiraishi, T., Qin, J., Konno, H., Akiyama, N., Shinzawa, M., Miyauchi, M., Takizawa, N., Yanai, H., Ohashi, H., Miyamoto-Sato, E., Yanagawa H., Yong, W., Shou, W., and Inoue, J.
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Journal Title
PLoS ONE
Volume: 9
Pages: 1-5
Related Report
Peer Reviewed
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[Journal Article] Mitochondria-nucleus shuttling FK506-binding protein 51 interacts with TRAF proteins and facilitates the RIG-I-like receptor-mediated expression of type I IFN
Author(s)
Akiyama, T., Shiraishi, T., Qin, J., Konno, H., Akiyama, N., Shinzawa, M., Miyauchi, M., Takizawa, N., Yanai, H., Ohashi, H., Miyamoto-Sato, E., Yanagawa H., Yong, W., Shou, W., and Inoue, J
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Journal Title
PLoS ONE
Volume: (in press)
Related Report
Peer Reviewed
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[Presentation] 次世代の個の医療に向けたがんオミクス解析による標的たんぱく質抽出と分子標的薬の設計2013
Author(s)
西川純一, 佐々木啓孝, 大橋広行, 平井直也, 與儀琢也, 清水孝恒, 山口類, 井元清哉, 鷲尾尊規, 山下辰博, 佐谷秀行, 宮野悟, 宮本悦子
Organizer
第36回日本分子生物学会年会
Place of Presentation
神戸ポートアイランド(兵庫県)
Year and Date
2013-12-03
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[Presentation] 統合的オミクス解析に基づく癌幹細胞の個性の理解と制御に向けて2012
Author(s)
宮本悦子, 藤森茂雄, 大橋広行, 平井直也, 清水孝恒, 佐谷秀行, 井本清哉, 山口類, 宮野悟, 森泰昌, 川畑順子, 増岡和代
Organizer
第71回日本癌学会学術総会
Place of Presentation
ロイトン札幌(北海道)
Year and Date
2012-09-19
Related Report
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