| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Research Abstract |
Cellular function is regulated by the balance of stringently regulated amounts of mRNA. Previous reports revealed that RNA polymerase II (RNAPII), which transcribes mRNA, c an be classified into the pausing state and the active transcription state according to the phosphorylation state of RPB1, the catalytic subunit of RNAPII. We examined the correlation between the phosphorylation of RNAPII and mRNA expression levels using acombined analysis by ChIPseq and RNAseq. We could visualized the recruitment of various phosphorylated RNAPIIs. Our analysis indicated that the complexity of quantitative regulation of mRNA levels could be classified into three categories according to thephosphorylation state of RNAPII.
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