| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
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| Research Abstract |
The length of the intron is widely diverse from under 100nucleotides to hundreds of thousands of nucleotides. Therefore, the lariat RNA intermediates, a by-product of splicing reaction, have various sizes. To sequence lariat RNA intermediates from cancer cells exhaustively using next generation sequencer, construction of high quality library from various size lariat RNA is essential. However, purification of high quality lariat RNA is very difficult because large lariat RNAs break easily biochemically and physically. We tested some RNA purification methods, and as a result, we find that handling very mildly during RNA purification like genomic DNA purification is very important for high quality lariat RNA purification. Now, we are trying to construct high quality lariat RNA library paying attention to this point.
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