Genome-wide analysis of chromatin structure at single molecule resolution by base modification and next-generation sequencing

Research Project

Project/Area Number	23651199
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Multi-year Fund
Research Field	System genome science
Research Institution	The University of Tokyo
Principal Investigator	ITO Takashi 東京大学, 大学院・理学系研究科, 教授 (90201326)
Project Period (FY)	2011 – 2012
Project Status	Completed (Fiscal Year 2012)
Budget Amount *help	¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000) Fiscal Year 2011: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Keywords	クロマチン構造 / 次世代シーケンサ / 次世代シーケンス / クロマチン / 修飾酵素プロービング
Research Abstract	To improve our understanding of chromatin dynamics, we have to reveal linkage/association among nucleosome/transcription factor?DNA interactions. Toward this goal, we tried to develop a novel method that utilizes base modifications, but not DNA cleavage, to reveal chromatin structure in a genome-wide scale. While a similar method termed NOME-Seq was reported, its combined use with PBAT, a bisulfite sequencing method of our own, would enable a practical and highly-sensitive analysis.

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