Genome-wide analysis of chromatin structure at single molecule resolution by base modification and next-generation sequencing
| Project/Area Number |
23651199
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
System genome science
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| Research Institution | The University of Tokyo |
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Principal Investigator |
ITO Takashi 東京大学, 大学院・理学系研究科, 教授 (90201326)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2011: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
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| Keywords | クロマチン構造 / 次世代シーケンサ / 次世代シーケンス / クロマチン / 修飾酵素プロービング |
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| Research Abstract |
To improve our understanding of chromatin dynamics, we have to reveal linkage/association among nucleosome/transcription factor?DNA interactions. Toward this goal, we tried to develop a novel method that utilizes base modifications, but not DNA cleavage, to reveal chromatin structure in a genome-wide scale. While a similar method termed NOME-Seq was reported, its combined use with PBAT, a bisulfite sequencing method of our own, would enable a practical and highly-sensitive analysis.
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Report
(3 results)
Research Products
(2 results)
