| Project/Area Number |
23651204
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
System genome science
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| Research Institution | National Institute of Genetics |
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Principal Investigator |
KANEMAKI Masato 国立遺伝学研究所, 新分野創造センター, 准教授 (20444507)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | タンパク質ネットワーク / タンパク質分解 / 植物ホルモン / 発現抑制 / 機能ゲノミクス / 細胞工学 |
|---|
| Research Abstract |
We have developed a novel protein depletion method, termed the auxin-inducible degron (AID) system, by transplanting a plant specific degradation pathway controlled by auxin. Because another plant hormone, Jasmonate-isoleucine (JA-ile), works in a similar way for degrading JAZ proteins in plants, we expected to make a new degron system in order to control two proteins independently by AID and JA-ile degrons. We tried to make a JA-ile degron system, but so far unfortunately, we have not succeeded yet. It seems to be necessary to revisit the working mechanism of JA-ile in plants and to optimize our assay conditions to test degradation of proteins using JA-ile. On the other hand, we have successfully improved the original AID system and applied for a patent
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