Creation of minimal artificial virus replication system by totally chemical syntheses
| Project/Area Number |
23651223
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Living organism molecular science
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| Research Institution | Tottori University (2012-2013)
Kyushu University (2011) |
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Principal Investigator |
MATSUURA Kazunori 鳥取大学, 工学(系)研究科(研究院), 教授 (60283389)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | ウイルス / DNA / ペプチド / 自己集合 / 無細胞発現系 / 合成ウイルス / 複製 / PCR |
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| Research Abstract |
The aim of this work is construction of minimal artificial virus that consists of synthetic short DNA and peptide nanocapsule (synthetic viral capsid). The important results are as follows: 1) We succeeded in construction of artificial virus that encapsulated M13 phage DNA in the beta-annulus peptide nanocapsule. 2) DNA that encoded beta-annulus peptide was designed, but the objective peptide could not be obtained by cell-free protein synthesis system. 3) DNA-modified artificial viral capsid was able to be constructed by self-assembly of DNA-beta-annulus peptide conjugate.
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Report
(4 results)
Research Products
(28 results)
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[Journal Article]2012
Author(s)
K. Matsuura
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Journal Title
Polymer J
Volume: 44
Issue: 6
Pages: 469-474
DOI
Related Report
Peer Reviewed
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