| Project/Area Number |
23651236
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Chemical biology
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| Research Institution | Osaka University |
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Principal Investigator |
OBIKA Satoshi 大阪大学, 大学院・薬学研究科, 教授 (80243252)
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| Co-Investigator(Kenkyū-buntansha) |
TACHIBANA Keisuke 大阪大学, 大学院・薬学研究科, 助教 (30432446)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 高機能性人工核酸 / BNA / アンチセンス分子 / スプライシング制御 / エクソンスキップ / 筋ジストロフィー / 家族性高コレステロール血症 |
|---|
| Research Abstract |
First, we established cell lines containing the reporter gene forantisense oligonucleotides that modify the RNA splicing. Next, we designed and analyzed various BNA antisense oligonucleotides using these screening cell lines, which allowed us to obtain several antisense oligonucleotides that induce exon skipping. We also identified three regions in the dystrophin gene as a good target for antisense oligonucleotide-mediated exon skipping.
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