| Project/Area Number |
23651249
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Resource conservation science
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| Research Institution | Mukogawa Women's University Junior College Division |
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Principal Investigator |
YOSHIDA Toru 武庫川女子大学短期大学部, 食生活学科, 准教授 (00378952)
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| Co-Investigator(Kenkyū-buntansha) |
FUKUO Keisuke 武庫川女子大学, 生活環境学部, 教授 (40156758)
FUKUDA Mitsuru 武庫川女子大学, 生活環境学部, 教授 (90098517)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 凍結保存 / 過冷却 / 磁場凍結 / アルテミア / 凍害防御剤 / トレハロース |
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| Research Abstract |
The cysts of Artemia franciscana (A. franciscana) were hydrated and their hatching rates were measured in order to estimate possible damagaes of cells and tissues by various freezing speeds. We observed that the hatching rates of hydrated cysts declined shortly at rapid frozen (submerged in liquid nitrogen), but high hatching rates maintained at slow freezing rate (0.5C/min), and that improved hatching ratio of decapsulated hydrated cysts by the treatment of cryoprotectants such as trehalose and dimethl sulfoxide (DMSO). We also evaluated that the surface of frozen hydrated cysts using a scanning electron microscope attached with cryo-transfer system (Cryo-SEM), and found fine structural discrepancy in the section of embryos between two freezing speeds.
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