• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Visualization of directional energy flow in a photosynthetic membrane by means of time-resolved super-resolution microsopy

Research Project

Project/Area Number 23654151
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Biophysics/Chemical physics
Research InstitutionOsaka City University

Principal Investigator

SUGISAKI Mitsuru  大阪市立大学, 大学院理学研究科, 准教授 (20360042)

Co-Investigator(Renkei-kenkyūsha) FUJII Ritsuko  大阪市立大学, 複合先端研究機構, 准教授 (80351740)
HASHIMOTO Hideki  大阪市立大学, 複合先端研究機構, 教授 (50222211)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywords顕微鏡 / 光合成 / 超解像度顕微鏡 / 超高速現象 / エネルギー問題 / ナノテクノロジー
Research Abstract

A microscope with a super-high spatial resolution, especially in the near infrared spectral region, has been constructed to elucidate the early events of photosynthesis, and its performance has been tested. Images taken using the conventional fluorescence microscope configuration were spatially diffraction-limited. However, it was found that a spatial resolution of around 100 nm has been attained when the super-resolution configuration was employed, which is limited by the mechanical steps of the sample stage of the microscope used in the experiment. An add-on unit for taking time-resolved micro-images was also contracted and its temporal resolution was measured.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (30 results)

All 2014 2013 2012 2011 Other

All Journal Article (9 results) (of which Peer Reviewed: 9 results) Presentation (20 results) Remarks (1 results)

  • [Journal Article] Ultrafast intramolecular relaxation dynamics of Mg- and Zn Bacteriochlorophyll a2013

    • Author(s)
      D. Kosumi
    • Journal Title

      J. Chem. Phys.

      Volume: 139 Issue: 3

    • DOI

      10.1063/1.4813526

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Generation of coherently coupled vibronic oscillations in carotenoids2012

    • Author(s)
      M. Sugisaki
    • Journal Title

      Physical Review B

      Volume: 85 Issue: 24 Pages: 245408

    • DOI

      10.1103/physrevb.85.245408

    • NAID

      120006770040

    • URL

      https://ocu-omu.repo.nii.ac.jp/records/2020403

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Excitation energy-transfer dynamics of brown algal photosynthetic antennas2012

    • Author(s)
      D. Kosumi
    • Journal Title

      Journal of Physical Chemistry Letters

      Volume: 3 Issue: 18 Pages: 2659-2664

    • DOI

      10.1021/jz300612c

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Ultrafast excited state dynamics of spirilloxanthin in solution and bound to core antenna complexes: identification of the S* and T1 states2012

    • Author(s)
      D. Kosumi
    • Journal Title

      Journal of Chemical Physics

      Volume: 137 Issue: 6 Pages: 64505-64505

    • DOI

      10.1063/1.4737129

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Excitation of Coherent Vibronic Oscillations in Carotenoid Molecules by Means of Four-Wave Mixing Spectroscopy : Intermolecular Coupling2011

    • Author(s)
      M. Sugisaki, D. Kosumi, K. Saito, R.J. Cogdell, and H. Hashimoto
    • Journal Title

      Carotenoid Science

      Volume: 16 Pages: 50-56

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Ultrafast excited state dynamics of fucoxanthin: excitation energy dependent intramolecular charge transfer dynamics2011

    • Author(s)
      D. Kosumi
    • Journal Title

      Phys. Chem. Chem. Phys.

      Volume: 13 Issue: 22 Pages: 10762-10770

    • DOI

      10.1039/c0cp02568b

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Excited state dynamics of spirilloxanthin in solution and bound to LH1-RC2011

    • Author(s)
      S. Maruta
    • Journal Title

      Carotenoid Science

      Volume: 16 Pages: 45-49

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Excitation of coherent vibronic oscillations in carotenoid molecules by means of four-wave mixing spectroscopy: interlevel coupling2011

    • Author(s)
      M. Sugisaki
    • Journal Title

      Carotenoid Science

      Volume: 16 Pages: 50-56

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Excitation energy dependent excited state dynamics of fucoxanthin2011

    • Author(s)
      D. Kosumi
    • Journal Title

      Carotenoid Science

      Volume: 16 Pages: 73-77

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] 超解像度顕微鏡の構築とその評価2014

    • Author(s)
      杉崎 満 , 小澄 大輔 , 橋本 秀樹
    • Organizer
      日本物理学会第69回年次大会, 平成25年度日本分光学会年次講演会
    • Place of Presentation
      東海大学
    • Related Report
      2013 Final Research Report
  • [Presentation] 超解像度顕微鏡の構築とその評価2014

    • Author(s)
      杉崎満
    • Organizer
      日本物理学会 第69回年次大会
    • Place of Presentation
      東海大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] Elucidation and control of functions of the marine photosynthetic pigment2013

    • Author(s)
      D. Kosumi
    • Organizer
      平成25年度 日本分光学会年次講演会
    • Place of Presentation
      大阪大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] 海洋カロテノイドにおける分子内電荷移動状態発現の共役鎖長依存性2013

    • Author(s)
      小澄大輔
    • Organizer
      第27回カロテノイド研究談話会
    • Place of Presentation
      三重大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] 有機合成によるフコキサンチンの機能制御2013

    • Author(s)
      小澄大輔
    • Organizer
      第27回カロテノイド研究談話会
    • Place of Presentation
      三重大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] 紅色光合成細菌アンテナにおける励起ダイナミク ス2012

    • Author(s)
      長岡悠也
    • Organizer
      日本物理学会 2012年秋季大会
    • Place of Presentation
      横浜国立大学常盤台キャンパス
    • Related Report
      2012 Research-status Report
  • [Presentation] 有機合成を用いた海洋カロテノイドにおける分子内電荷移動の機能制御2012

    • Author(s)
      小澄 大輔
    • Organizer
      日本物理学会第67回年次大会
    • Place of Presentation
      関西学院大学上ヶ原キャンパス
    • Related Report
      2011 Research-status Report
  • [Presentation] カロテノイドにおける超高速光学応答のヘテロダイン検出2011

    • Author(s)
      杉崎 満, 小澄 大輔 , 橋本 秀樹
    • Organizer
      日本物理学会 2011年秋季大会
    • Place of Presentation
      富山大学
    • Related Report
      2013 Final Research Report
  • [Presentation] A new ultrafast energy dissipation pathway found in a purple bacterial photosynthetic light-harvesting system2011

    • Author(s)
      H. Hashimoto
    • Organizer
      International Workshop "Mechanisms of Non-photochemical Quenching"(招待講演)
    • Place of Presentation
      Passau, Germany
    • Related Report
      2011 Research-status Report
  • [Presentation] Energy-transfer dynamics between carotenoid and bacteriochlorophyll in the light-harvesting system of purple photosynthetic bacteria as visualized by sub-picosecond pump-and probe and sub-20 fs four-wave mixing spectroscopies2011

    • Author(s)
      H. Hashimoto
    • Organizer
      Light-Harvesting Processes (LHP) 2011(招待講演)
    • Place of Presentation
      Banz, Germany
    • Related Report
      2011 Research-status Report
  • [Presentation] Excitation energy dependence of intramolecular charge transfer dynamics of fucoxanthin2011

    • Author(s)
      D. Kosumi
    • Organizer
      The 16th International Symposium on Carotenoids
    • Place of Presentation
      Krakow, Poland
    • Related Report
      2011 Research-status Report
  • [Presentation] 紅色光合成細菌の光捕集アンテナにおけるカロテノイド―バクテリオクロロフィル間のエネルギー移動メカニズム2011

    • Author(s)
      丸田 聡
    • Organizer
      第25回カロテノイド研究談話会
    • Place of Presentation
      つくば国際会議場
    • Related Report
      2011 Research-status Report
  • [Presentation] 発現条件の異なる紅色光合成細菌アンテナ系LH2, LH3における色素間励起エネルギー移動2011

    • Author(s)
      長岡 悠也
    • Organizer
      第25回カロテノイド研究談話会
    • Place of Presentation
      つくば国際会議場
    • Related Report
      2011 Research-status Report
  • [Presentation] フェムト秒時間分解分光による褐藻類光合成エネルギー伝達の解明2011

    • Author(s)
      小澄 大輔
    • Organizer
      第25回カロテノイド研究談話会
    • Place of Presentation
      つくば国際会議場
    • Related Report
      2011 Research-status Report
  • [Presentation] 発現条件の異なる紅色光合成細菌アンテナ系LH2, LH3におけるフェムト秒分光2011

    • Author(s)
      長岡 悠也
    • Organizer
      日本物理学会2011年秋季大会
    • Place of Presentation
      富山大学
    • Related Report
      2011 Research-status Report
  • [Presentation] 海洋カロテノイドにおける分子内電荷移動の共役鎖長依存性2011

    • Author(s)
      小澄 大輔
    • Organizer
      日本物理学会2011年秋季大会
    • Place of Presentation
      富山大学
    • Related Report
      2011 Research-status Report
  • [Presentation] 紅色細菌由来の集光アンテナにおけるバクテリオクロロフィルの線形・非線形光学応答2011

    • Author(s)
      長岡 悠也
    • Organizer
      日本物理学会2011年秋季大会
    • Place of Presentation
      富山大学
    • Related Report
      2011 Research-status Report
  • [Presentation] 紅色光合成細菌の光防護作用から見るカロテノイド―バクテリオクロロフィル間のエネルギー移動メカニズム2011

    • Author(s)
      丸田 聡
    • Organizer
      日本物理学会2011年秋季大会
    • Place of Presentation
      富山大学
    • Related Report
      2011 Research-status Report
  • [Presentation] 褐藻類光合成アンテナの異なるサイトに結合したフコキサンチンの励起状態ダイナミクス2011

    • Author(s)
      小澄 大輔
    • Organizer
      日本物理学会2011年秋季大会
    • Place of Presentation
      富山大学
    • Related Report
      2011 Research-status Report
  • [Presentation] カロテノイドにおける超高速光学応答のヘテロダイン検出2011

    • Author(s)
      杉崎 満
    • Organizer
      日本物理学会2011年秋季大会
    • Place of Presentation
      富山大学
    • Related Report
      2011 Research-status Report
  • [Remarks]

    • URL

      http://www.sci.osaka-cu.ac.jp/phys/PBM/index-j.html

    • Related Report
      2013 Final Research Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi