Technical development of selective observation of proteins inliving cells by using ultraviolet resonance Raman spectroscopy
| Project/Area Number |
23655012
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Physical chemistry
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| Research Institution | Osaka University |
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Principal Investigator |
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| Research Collaborator |
ISHIKAWA Haruto 大阪大学, 大学院・理学研究科, 講師 (40551338)
MIZUNO Misao 大阪大学, 大学院・理学研究科, 助教 (10464257)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 共鳴ラマン分光法 / in-vivo測定 / タンパク質構造 |
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| Research Abstract |
In order to elucidate functional mechanism based on molecular structure of proteins, it is important to study not only purified proteins but also proteins in living cells. In this project, we developed measurement techniques to selectively observe Raman spectra of proteins in living cells with high sensitivity. We investigated dependences of overexpressed proteins and extent of overexpression on ultraviolet resonance Raman spectra of living cells.
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Report
(3 results)
Research Products
(4 results)
