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Electrochemical regulation of nucleic acids hybridization

Research Project

Project/Area Number 23655068
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Analytical chemistry
Research InstitutionKumamoto University

Principal Investigator

IHARA Toshihiro  熊本大学, 自然科学研究科, 教授 (40253489)

Co-Investigator(Renkei-kenkyūsha) OBIKA Satoshi  大阪大学, 大学院・薬学研究科, 教授 (80243252)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywords生体分析 / 人工核酸 / 電気化学 / 電極修飾 / DNAセンサー / 電気化学的制御 / DNAコンジュゲート / 核酸 / 分子認識 / センサー / フェロセン / 金属錯体 / ターピリジン / BNA / LNA / 協同性 / 錯生成 / ハイブリダイゼーション
Research Abstract

I have been trying to immobilize modified DNA carrying disulfide-bridged deoxyribose (ssDNA) and two terpyridine units (terpy2DNA) on carbon and gold electrode, respectively. I expect that I can monitor the hybridization by the change in their electrochemical properties.
ssDNA was immobilized on HPOG electrode through stacking interaction of aromatic molecules such as pyrene and perylene onto the HOPG. terpy2DNA was immobilized on Au electrode by using specific affinity of sulfhydryl group, which was modified at the end of terpy2DNA, to Au. Unfortunately, the electrochemical signal of sulfhydryl/disulfide redox pair in ssDNA was not observed. The potential would be out of the measurement window. terpy2DNA formed intramolecular 1:2 complex with some divalent transition metal ions to form [Fe(terpy)2]2+. The redox profile of [Fe(terpy)2]2+/3+ on terpy2DNA was clearly observed on the Au electrode. The hybridization of terpy2DNA was monitored by frequency dependence on SWV measurement.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (28 results)

All 2014 2013 2012 2011 Other

All Journal Article (10 results) (of which Peer Reviewed: 10 results,  Open Access: 1 results) Presentation (14 results) (of which Invited: 6 results) Book (3 results) Remarks (1 results)

  • [Journal Article] Potentiometric DNA Sensing Platform Using Redox-Active DNA Probe Pair for Sandwich-Type Dual Hybridization at Indicator Electrode Surface2014

    • Author(s)
      K. Nakano, T. Kimura, Y. Kitamura, T. Ihara, R. Ishimatsu, T. Imato
    • Journal Title

      J. Electroanal. Chem

      Volume: 720-721 Pages: 71-75

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] In Situ Oxygenous Functionalization of a Graphite Electrode for Enhanced Affinity toward Charged Species and a Reduced Graphene Oxide Mediator2014

    • Author(s)
      M. R. Karim, Y. Ikeda, T. Ide, S. Sugimoto, K. Toda, Y. Kitamura, T. Ihara, T. Matsui, T. Taniguchi, M. Koinuma, Y. Matsumoto, S. Hayami
    • Journal Title

      New J. Chem

      Volume: 38 Pages: 2120-2127

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Versatile Allosteric Molecular Devices Based on Reversible Formation of Luminous Lanthanide Complexes2013

    • Author(s)
      Y. Kitamura, S. Yamamoto, Y. Osawa, H. Matsuura, T. Ihara
    • Journal Title

      Chem. Commun

      Volume: 49 Pages: 285-287

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Metallo-regulation of the Bimolecular Triplex Formation of a Peptide2013

    • Author(s)
      H. Shimada, T. Sakurai, Y. Kitamura, H. Matsuura, T. Ihara
    • Journal Title

      Nucleic Acid Dalton Trans

      Volume: 42 Pages: 16006-16013

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] DNA Analysis Based on Toehold-mediated Strand Displacement on Graphene Oxide2013

    • Author(s)
      T. Miyahata, Y. Kitamura, A. Futamura, H. Matsuura, K. Hatakeyama, M. Koinuma, Y. Matsumoto, T. Ihara
    • Journal Title

      Chem. Commun

      Volume: 49 Pages: 10139-10141

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Versatile Allosteric Molecular Devices Based on Reversible Formation of Luminous Lanthanide Complexes2013

    • Author(s)
      Y. Kitamura, S. Yamamoto, Y. Osawa, H. Matsuura, T. Ihara
    • Journal Title

      Chem. Commun.

      Volume: 49 Issue: 3 Pages: 285-287

    • DOI

      10.1039/c2cc36979f

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Metallo-regulation of the bimolecular triplex formation of a peptide nucleic acid.2013

    • Author(s)
      Shimada H, Sakurai T, Kitamura Y, Matsuura H, Ihara T
    • Journal Title

      Dalton Trans.

      Volume: 42(45) Issue: 45 Pages: 16006-13

    • DOI

      10.1039/c3dt51386f

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Rational design for cooperative recognition of specific nucleobases using β-cyclodextrin-modified DNAs and fluorescent ligands on DNA and RNA scaffolds2013

    • Author(s)
      A. Futamura, A. Uemura, T. Imoto, Y. Kitamura, H. Matsuura, C. -X. Wang, T. Ichihashi, Y. Sato, N. Teramae, S. Nishizawa, T. Ihara
    • Journal Title

      Chemistry, A European Journal

      Volume: 19 Issue: 32 Pages: 10526-10535

    • DOI

      10.1002/chem.201300985

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] スプリット型プローブの協同的錯体形成を利用するDNAの認識及び検出2012

    • Author(s)
      井原敏博、北村裕介
    • Journal Title

      分析化学

      Volume: 61 Pages: 193-206

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Electrochemical Signal Modulation in Homogeneous Solutions Using the Formation of an Inclusion Complex between Ferrocene and β-cyclodextrin on DNA Scaffold2011

    • Author(s)
      T. Ihara, T. Wasano, R. Nakatake, P. Arslan, A. Futamura, A. Jyo
    • Journal Title

      Chem. Commun

      Volume: 47 Pages: 12388-12390

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Presentation] 金属配位構造を骨格に導入した DNA による機能性核酸の制御2014

    • Author(s)
      大浦博之, 白浜千里, 古園智大, 北村裕介, 井原敏博
    • Organizer
      日本化学会第94春季年会
    • Place of Presentation
      名古屋大学東山キャンパス
    • Year and Date
      2014-03-27
    • Related Report
      2013 Final Research Report
  • [Presentation] Ion-directed Drastic Conformational Change of DNA and Its Application to DNAzyme Activity Control2013

    • Author(s)
      H. Ohura, T. Furuzono, C. Shirahama, Y. Kitamura, T. Ihara
    • Organizer
      第40回国際核酸化学シンポジウム
    • Place of Presentation
      神奈川大学
    • Year and Date
      2013-11-13
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] 刺激応答性を有する DNA2013

    • Author(s)
      井原敏博
    • Organizer
      バイオマテリアル学会九州講演会2013
    • Place of Presentation
      熊本大学黒髪北キャンパス
    • Year and Date
      2013-09-20
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] Flexible biomolecular sensor based on reversible formation of luminescent lanthanide complexes by metal chelator-DNA conjugates2013

    • Author(s)
      Y. Kitamura, S. Yamamoto, R. Ozaki, T. Ihara
    • Organizer
      The Twelfth Asian Conference on Analytical Sciences (ASIANALYSIS XII)
    • Place of Presentation
      九州大学馬出キャンパス
    • Year and Date
      2013-08-22
    • Related Report
      2013 Final Research Report
  • [Presentation] 核酸上でのデザインされた分子間相互作用およびその分析化学的応用2013

    • Author(s)
      井原敏博
    • Organizer
      生命理工学研究科セミナー
    • Place of Presentation
      東京工業大学
    • Year and Date
      2013-07-09
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] DNA-directed metal complex formations and analytical applications2013

    • Author(s)
      T. Ihara
    • Organizer
      Asian International Symposium
    • Place of Presentation
      立命館大学びわこ・くさつキャンパス
    • Year and Date
      2013-03-24
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] 刺激応答性を有するDNA2013

    • Author(s)
      井原敏博
    • Organizer
      バイオマテリアル学会九州講演会2013
    • Place of Presentation
      熊本大学
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] シクロデキストリンをシグナル調節因子とするバイオ分析2013

    • Author(s)
      井元千春、二村朱香、北村裕介、井原敏博
    • Organizer
      第30回シクロデキストリンシンポジウム
    • Place of Presentation
      くまもと県民交流館パレア
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 電気化学的モレキュラービーコンの開発とその遺伝子検出への応用2013

    • Author(s)
      井元千春、見汐航太朗、北村裕介、井原敏博
    • Organizer
      第30回シクロデキストリンシンポジウム
    • Place of Presentation
      くまもと県民交流館パレア
    • Related Report
      2013 Annual Research Report
  • [Presentation] 電気化学的モレキュラービーコンの開発2013

    • Author(s)
      井元千春、見汐航太朗、中武隆太、北村裕介、井原敏博
    • Organizer
      第50回化学関連支部合同九州大会
    • Place of Presentation
      北九州国際会議場
    • Related Report
      2013 Annual Research Report
  • [Presentation] Versatile Molecular Devices Based on the Reversible Formation of Luminous Lanthanide Complexes2012

    • Author(s)
      Y. Kitamura, S. Yamamoto, Y. Osawa, T. Ihara
    • Organizer
      The 39th International Symposium on Nucleic Acids Chemistry
    • Place of Presentation
      名古屋大学豊田講堂
    • Year and Date
      2012-11-15
    • Related Report
      2013 Final Research Report
  • [Presentation] π-π相互作用を利用した炭素電極上への DNA 固定化に関する研究2012

    • Author(s)
      森本啓示, 松山泰知, 北村裕介, 井原敏博
    • Organizer
      第49回化学関連支部合同九州大会
    • Place of Presentation
      北九州市国際会議場
    • Year and Date
      2012-06-30
    • Related Report
      2013 Final Research Report
  • [Presentation] DNA上でのフェロセン-シクロデキストリン相互作用を利用した均一溶液中での電気化学的核酸分析法に関する基礎研究2012

    • Author(s)
      井原敏博、中武隆太、見汐航太郎、北村裕介
    • Organizer
      第72回分析化学討論会
    • Place of Presentation
      鹿児島大学工学部
    • Related Report
      2012 Research-status Report
  • [Presentation] 電気化学的モレキュラービーコンの開発2012

    • Author(s)
      見汐航太朗、中武隆太、北村裕介、井原敏博
    • Organizer
      第49回化学関連支部合同九州大会
    • Place of Presentation
      北九州国際会議場
    • Related Report
      2012 Research-status Report
  • [Book] "金属錯体の特異的な形成および相互作用を利用した新規核酸プローブの開発", "Development of Novel Nucleic Acid Probes Based on the Template-directed Formation of Metal Complexes"2013

    • Author(s)
      北村裕介, 井原敏博
    • Publisher
      分析化学
    • Related Report
      2013 Final Research Report
  • [Book] Photochemically Relevant DNA-based Molecular Systems Enabling Chemical and Signal Transductions and Their Analytical Applications2012

    • Author(s)
      T. Ihara, Y. Kitamura
    • Publisher
      J. Photochem. Photobiol. C : Photochem. Rev
    • Related Report
      2013 Final Research Report
  • [Book] "核酸とカチオンの特異な相互作用", CSJ カレントレビュー 核酸化学のニュートレンド2011

    • Author(s)
      井原敏博(共同執筆)
    • Publisher
      化学同人
    • Related Report
      2013 Final Research Report
  • [Remarks]

    • URL

      http://133.95.131.186/~toshi/

    • Related Report
      2013 Final Research Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

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