Emergence of protein-like higher order structures and functions by dynamic reorganization of polypeptide side chains

• Project/Area Number: 23655099
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Polymer chemistry
• Research Institution: Yokohama National University
• Principal Investigator: OYAMA Toshiyuki 横浜国立大学, 工学研究院, 准教授 (30313472)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 動的共有結合 / ポリペプチド / イミン / アルデヒド / 高次構造 / CDスペクトル / 動的組換え

Research Abstract

A polypeptide to which a functional group derived from an aldehyde was introduced via imine bond was prepared by the reaction of the aldehyde with amino groups of poly(L-lysine) having a small amount of hydrophilic side chains. The polypeptide was dissolved in methanol along with aldehydes with various functional groups, and shuffling of side chain functional groups based on dynamic exchange between imine and aldehyde was observed. Higher-order structure of the polypeptide was found to be changed with the side-chain shuffling.

Research Products

• [Presentation] イミン結合を利用したポリペプチド側鎖の動的組換え反応 (2013)
  • Author(s): 大山俊幸(他2名)
  • Organizer: 日本化学会第93春季年会
  • Place of Presentation: 立命館大学びわこ・くさつキャンパス(滋賀県草津市野路東1丁目1-1)
  • Year and Date: 2013-03-25
• [Presentation] Introduction of various functional groups onto the side chains of polypeptides using dynamic exchange reaction mediated by imine bonds (2012)
  • Author(s): 大山俊幸(他2名)
  • Organizer: 1st International Conference on Emerging Advanced Nanomaterilas (ICEAN2012)
  • Place of Presentation: メルキュールホテル(ブリスベン,オーストラリア)
  • Year and Date: 2012-10-22
• [Presentation] イミン結合の動的結合組換えを利用したポリペプチド鎖への種々の官能基の導入 (2012)
  • Author(s): 大山俊幸(他2名)
  • Organizer: 第61回高分子学会年次大会
  • Place of Presentation: シフィコ横浜(横浜市西区みなとみらい1-1-1)
  • Year and Date: 2012-05-30
• [Presentation] 側鎖に導入したイミン結合の動的組換えがポリペプチド主鎖の高次構造に及ぼす影響 (2012)
  • Author(s): 遠藤慶一郎、高橋昭雄、大山俊幸
  • Organizer: 第６０回高分子討論会
  • Place of Presentation: 岡山大学（岡山県）
• [Presentation] 側鎖に導入したイミン結合の動的組換えがポリペプチド主鎖の高次構造に及ぼす影響 (2011)
  • Author(s): 大山俊幸(他2名)
  • Organizer: 第60回高分子討論会
  • Place of Presentation: 山大学津島キャンパス(岡山市北区津島中2-1-1)
  • Year and Date: 2011-09-30
• [Presentation] イミン結合の動的結合組換えを利用したポリペプチド鎖への種々の官能基の導入
  • Author(s): 遠藤慶一郎（大山俊幸）
  • Organizer: 第６１回高分子学会年次大会
  • Place of Presentation: パシフィコ横浜（神奈川県）
• [Presentation] Introduction of various functional groups onto the side chains of polypeptides using dynamic exchange reaction mediated by imine bonds
  • Author(s): 遠藤慶一郎（大山俊幸）
  • Organizer: 1st International Conference on Emerging Advanced Nanomaterials (ICEAN2012)
  • Place of Presentation: Mercure Hotel (Brisbane, Australia)
• [Presentation] イミン結合を利用したポリペプチド側鎖の動的組換え反応
  • Author(s): 大山俊幸（大山俊幸）
  • Organizer: 日本化学会第93春季年会
  • Place of Presentation: 立命館大学びわこ・くさつキャンパス（滋賀県）