• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Construction and analysis of the evaluation system of environmental chemical substances on longevity using a longevity gene as an index of assessment

Research Project

Project/Area Number 23655139
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Environmental chemistry
Research InstitutionHokkaido University

Principal Investigator

KURASAKI Masaaki  北海道大学, 大学院・地球環境科学研究院, 助教 (80161727)

Co-Investigator(Kenkyū-buntansha) SAITO Takeshi  北海道大学, 大学院・保健科学研究院, 教授 (40153811)
Co-Investigator(Renkei-kenkyūsha) SATO Shin  青森県立保健大学, 健康科学部, 教授 (40310099)
Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords微量環境物質評価 / 影響評価法 / 長寿遺伝子 / エピジェネチック / リアルタイムPCR / アポトーシス / フタル酸ジエチル / PC12細胞 / 微量環境汚染化学物質 / サーチュインファミリー
Research Abstract

To construct a new evaluation system for environmental contaminants, we have examined effects of chemical contaminants on expression of sirtuin family using PC12 cell system. As results, expression of Sirt 1 was significantly up-regulated by treatment of diethyl phthalate (one of the potential endocrine disrupters and enhance element of apoptosis in the cell), copper or hydrogen peroxide in PC12 cells. In addition, the tendency that expression of Sirt1 and Sirt2 was decreased in the rat offspring from undernourished pregnant mothers was observed. From the results, it was concluded that measurement of Sirt1 and Sirt2 were useful tool for evaluation of chemical substances on organism.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (16 results)

All 2013 2012 2011 Other

All Journal Article (9 results) (of which Peer Reviewed: 9 results) Presentation (7 results)

  • [Journal Article] Diethyl phthalate enhances expression of SIRT1, DNMT3a and DNMT3b during apoptosis in PC12 Cells2013

    • Author(s)
      Sun Y, Guo Z, Iku S, Saito T and Kurasaki M
    • Journal Title

      Journal of Applied Toxicology

      Volume: (in press) Issue: 12 Pages: 1484-1492

    • DOI

      10.1002/jat.2816

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects of parabens on apoptosis induced by serum deprivation in PC12 cells2012

    • Author(s)
      Mari Egawa, Kentaro Aoki, Yongkun Sun, Toshiyuki Hosokawa, Takeshi Saito and Masaaki Kurasaki
    • Journal Title

      Journal of Environmental Science and Health, Part B. Pesticides, Food Contaminants, and Agricultural Wastes

      Volume: Vol.47, No. 3 Pages: 196-204

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Diethyl phthalate enhances apoptosis induced by serum deprivation inPC12 cells2012

    • Author(s)
      Sun Y, Takahashi K, Saito T, Hosokawa T, Kurasaki M
    • Journal Title

      Basic & Clinical Pharmacology& Toxicology

      Volume: 111 Issue: 2 Pages: 113-119

    • DOI

      10.1111/j.1742-7843.2012.00869.x

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects of parabens on apoptosis induced by serum-free medium2012

    • Author(s)
      Egawa M, Aoki K, Sun Y, Hosokawa T, Saito T and Kurasaki M
    • Journal Title

      Journal of Environmental Science and Health

      Volume: 47 Issue: 3 Pages: 196-204

    • DOI

      10.1080/03601234.2012.634341

    • NAID

      120005303468

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Stevioside enhances apoptosis induced by serum deprivation in PC12 cells2012

    • Author(s)
      Takahashi K, Sun Y, Yanagiuchi I, Hosokawa T, Saito T, Komori M, Okino T and Kurasaki M
    • Journal Title

      Toxicology Mechanisms and Methods

      Volume: 22 Issue: 4 Pages: 243-249

    • DOI

      10.3109/15376516.2012.658978

    • NAID

      120005228672

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Bisphenol-A Suppresses Neurite Extension due to Inhibition of Phosphorylation of Mitogenactivated Protein Kinase in PC12 Cells2011

    • Author(s)
      Yuuka Mukai, Yongkun Sun and Shin Sato, Azuki bean polyphenols intake duringMiyako Komori and Masaaki Kurasaki
    • Journal Title

      Chemico-Biological Interactions

      Volume: Vol. 194, No. 1 Pages: 23-30

    • NAID

      120003483496

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Bisphenol-A suppresses neurite extension due to inhibition of phosphorylation of mitogen-activated protein kinase in PC12 cells2011

    • Author(s)
      Seki S, Aoki M, Hosokawa T, Saito T, Masuma R, Komori M and Kurasaki M
    • Journal Title

      Chemico-Biological Interactions

      Volume: 194 Issue: 1 Pages: 23-30

    • DOI

      10.1016/j.cbi.2011.08.001

    • NAID

      120003483496

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Diethyl phthalate enhances expression of SIRT1 and DNMT3a during apoptosis in PC12 Cells

    • Author(s)
      Yongkun Sun, Zhikun Guo, Shouhei Iku, Takeshi Saito and Masaaki Kurasaki
    • Journal Title

      Journal of Applied Toxicology

      Volume: (in press)

    • NAID

      120005341790

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Diethyl phthalate enhances expression of SIRT1 and DNMT3a during apoptosis in PC12 Cells

    • Author(s)
      Yongkun Sun, Zhikun Guo, Shouhei Iku, Takeshi Saito and MasaakiKurasak i
    • Journal Title

      Journal of Applied Toxicology

      Volume: (in press)

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Presentation] Effect of quercetin intake during lactation on the AMPK activation in the hypothalamus of adult male rat offspring programmed by maternal protein restriction2012

    • Author(s)
      Masato Tanaka, Mai Tsuruga, Norihito Takahashi, Aya Sasaki, Miki Miyajima, Ryo Nishimura, Naoko Hishioka, Yuuka Mukai, Shin Sato, Masaaki Kurasaki and Takeshi Saito
    • Organizer
      The 6th International Niigata Symposium on Diet and Health
    • Place of Presentation
      Toki Messe (Niigata)
    • Year and Date
      2012-10-16
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Presentation] Effects of consumption of green tea during lactation on epigenetic factors in female infants of pregnancy rats with low-protein diet2012

    • Author(s)
      Yongkun Sun, Yuuka Mukai, Shin Sato, Toshiyuki Hosokawa, Takeshi Saito and Masaaki Kurasaki
    • Organizer
      The 6th International Niigata Symposium on Diet and Health
    • Place of Presentation
      Toki Messe (Niigata)
    • Year and Date
      2012-10-16
    • Related Report
      2012 Final Research Report
  • [Presentation] Changes of epigenetic factors in apoptotic status induced by copper in PC12 cells2012

    • Author(s)
      Yongkun Sun, Miki Miyajima, Toshiyuki Hosokawa, Takeshi Saito, Masaaki Kurasaki
    • Organizer
      8th International Copper Meeting; Copper in Biology
    • Place of Presentation
      Hotel Calabona (Italy)
    • Year and Date
      2012-10-02
    • Related Report
      2012 Final Research Report
  • [Presentation] 妊娠期緑茶投与の出産仔のエピゲネチック因子に及ぼす影響2012

    • Author(s)
      孫永〓、佐藤伸、向井友花、細川敏幸、齋藤健、蔵崎正明
    • Organizer
      第82回日本衛生学会
    • Place of Presentation
      京都大学(京都市)
    • Year and Date
      2012-03-25
    • Related Report
      2012 Final Research Report
  • [Presentation] 妊娠期緑茶投与の出産仔のエピゲネチック因子に及ぼす影響2012

    • Author(s)
      孫永コン、佐藤 伸、向井友花、細川敏幸、齋藤健、蔵崎正明
    • Organizer
      第82回日本衛生学会
    • Place of Presentation
      京都大学 (京都市)
    • Year and Date
      2012-03-25
    • Related Report
      2011 Research-status Report
  • [Presentation] Effects of Consumption of Green Tea during Lactation on Epigenetic Factors in Female Infants of Pregnancy Rats with Low-protein Diet2012

    • Author(s)
      Yongkun Sun et al.
    • Organizer
      The 6th International Niigata Symposium on Diet and Health
    • Place of Presentation
      Toki Messe (Niigata)
    • Related Report
      2012 Annual Research Report
  • [Presentation] A Novel Study of Effects of Environmental Chemical Substances on Epigenetic Modification2011

    • Author(s)
      Yongkun Sun, Toshiyuki Hosokawa, Takeshi Saito and Masaaki Kurasaki
    • Organizer
      Cell Symposia: Epigenetics and the Inheritance of Acquired States 2011
    • Place of Presentation
      The Westin Boston Waterfront (USA)
    • Year and Date
      2011-10-11
    • Related Report
      2012 Final Research Report 2011 Research-status Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi