| Project/Area Number |
23655146
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Environmental chemistry
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| Research Institution | Tokyo University of Science |
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Principal Investigator |
YOSHIMURA Takeo 東京理科大学, 理工学部・応用生物科学科, 助教 (10580938)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | グリーンケミストリー / マイクロ波 / 無細胞 / 遺伝子増幅 / 無細胞蛋白質発現 |
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| Research Abstract |
New technology of protein expression is necessary for protein crystal structure, biomaterial and medical science. The cell free protein expression system is expected to lead the future bioengineering. To achieve acceleration of activity of enzymatic reactions, we prepared an original microwave applicator for microliter reaction scale. In this study, expression of GFP protein and DNA polymerization were demonstrated by using resonant cavity type of microwave applicator. As a result, we confirmed the microwave effectto enzymatic reactions.
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