| Project/Area Number |
23655152
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Chemistry related to living body
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| Research Institution | The University of Tokyo |
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Principal Investigator |
SATO Moritoshi 東京大学, 大学院・総合文化研究科, 准教授 (00323501)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 生体機能関連化学 / 生物発光 / 細胞 / 生体 / イメージング / プローブ / ルシフェラーゼ / ガン細胞 / 転移 / 可視化 / エネルギー移動 / 蛍光 |
|---|
| Research Abstract |
In this study, a rational development of superluminescent variants of a luciferase derived from Gaussia princeps was demonstrated. A putative catalytic center of Gaussia luciferase was assigned and modified by a site-directed mutagenesis. The potent variants were found to generate up to 10 times stronger bioluminescence, emitting red shifts of up to 33 nm with natural coelenterazine than native GLuc, rendering an efficient optical signature in bioassays. The advantageous properties were demonstrated with metastases of murine B16 melanoma in BALB/c nude mice.
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