Exploring novel DNA modification toward paradigm shift of epigenetics
Project/Area Number |
23655153
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Chemistry related to living body
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Research Institution | The University of Tokyo |
Principal Investigator |
SUZUKI Tsutomu 東京大学, 大学院・工学系研究科, 教授 (20292782)
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Project Period (FY) |
2011 – 2012
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Project Status |
Completed (Fiscal Year 2012)
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Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
|
Keywords | 核酸 / 蛋白質 / 糖化学 / DNA修飾 / マススペクトロメトリー / エピゲノム / エピジェネティックス |
Research Abstract |
Alteration of chromatin structure caused by DNA methylation and histone modifications is closely associated with higher-ordered biological processes including development, differentiation and diseases by regulating gene expression epigenetically. In this study, we employed a high-sensitive analytical method using mass spectrometry to identify some novel DNA modified bases in mammalian genomes. The chemical structure of one of them was determined.
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Report
(3 results)
Research Products
(73 results)
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[Journal Article] Decoding system for the AUA codon by tRNAIle with the UAU anticodon in Mycoplasma mobile2013
Author(s)
aniguchi, T., Miyauchi, K., Nakane, D., Miyata, M., Muto, A., Nishimura, S. and Suzuki, T.
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Journal Title
Nucleic Acids Res.
Volume: 41
Pages: 2621-2631
Related Report
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[Journal Article] Yokobori, S., Yamagishi, A. and Watanabe, K.* Decoding mechanism of non-universal genetic codes in Loligo bleekeri mitochondria2013
Author(s)
Ohira, T., Suzuki, T., Miyauchi, K., Suzuki, T.
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Journal Title
J. Biol. Chem.
Volume: 288
Pages: 7645-7652
Related Report
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[Journal Article] Crystal structure of a putative methyltransferase SAV1081 from Staphylococcus aureus2013
Author(s)
Kita, S., Tanaka, Y., Hirano, N., Kimura, S., Suzuki, T., Suzuki, T., Yao, M. and Tanaka, I.
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Journal Title
Protein & Peptide Letters
Volume: 20
Pages: 530-537
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[Journal Article] Structure-function analysis of human TYW2 enzyme required for the biosynthesis of a highly modified wybutosine (yW) base inphenylalanine-tRNA2012
Author(s)
Rodriguez, V., Vasudevan, S., Noma, A., Carlson, B.A., Green, J.E., Suzuki, T. and Chandrasekharappa, SC.
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Journal Title
Related Report
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[Journal Article] LRPPRC/SLIRP suppresses PNPase-mediated mRNA decay and promotes polyadenylation in human mitochondria.2012
Author(s)
Chujo, T., Ohira, T., Sakaguchi, Y., Goshima, N., Nomura, N., Nagao, A. and Suzuki, T.
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Journal Title
Nucleic Acids Res.
Volume: 40
Pages: 8033-8047
Related Report
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[Journal Article] Loss of snoRNA impairs ribosomal RNA modification and causes developmental defects in zebrafish2012
Author(s)
Higa-Nakamine, S., Suzuki, T., Uechi, T., Chakraborty, A., Nakajima, Y., Nakamura, M., Hirano, N., Suzuki, T. and Kenmochi, N.
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Journal Title
Nucleic Acids Res.
Volume: 40
Pages: 391-398
Related Report
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[Journal Article] Base methylations in the double-stranded RNA by a fused methyltransferase bearing unwinding activity2012
Author(s)
Kimura, S., Ikeuchi, Y., Kitahara, K., Sakaguchi, Y., Suzuki, T. and Suzuki, T.
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Journal Title
Nucleic Acids Res.
Volume: 40
Pages: 4071-4085
Related Report
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[Journal Article] Structure-function analysis of human TYW2 enzyme required for the biosynthesis of a highly modified wybutosine (yW) base in phenylalanine-tRNA.2012
Author(s)
Rodriguez, V., Vasudevan, S., Noma, A., Carlson, B.A., Green, J.E., Suzuki, T. and Chandrasekharappa, SC.
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Journal Title
PLoS ONE
Volume: 7
Issue: 6
Pages: e39297-e39297
DOI
Related Report
Peer Reviewed
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[Journal Article] LRPPRC/SLIRP suppresses PNPase-mediated mRNA decay and promotes polyadenylation in human mitochondria.2012
Author(s)
Chujo, T., Ohira, T., Sakaguchi, Y., Goshima, N., Nomura, N., Nagao, A. and Suzuki, T.
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Journal Title
Nucleic Acids Res.
Volume: 40
Issue: 16
Pages: 8033-8047
DOI
Related Report
Peer Reviewed
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[Journal Article] Molecular basis of dihydrouridine formation on tRNA2011
Author(s)
Yu, F., Tanaka, Y., Yamashita, K., Suzuki, T., Nakamura, A., Hirano, N., Suzuki, T., Yao, M. and Tanaka, I.
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Journal Title
Proc. Natl. Acad. Sci. USA.
Volume: 108
Pages: 19593-19598
Related Report
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[Journal Article] Crystallization and preliminary X-ray diffraction analysis of an archaeal tRNA modificationenzyme TiaS complexed with tRNAIle2 and ATP2011
Author(s)
Osawa, T., Inanaga, H., Kimura, S., Terasaka, N., Suzuki, T. and Numata, T.
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Journal Title
Acta Crystallograph Sect F Struct Biol Cryst Commun.
Volume: 67
Pages: 1414-1416
Related Report
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[Journal Article] Structural basis of tRNA agmatinylation essential for AUA codon decoding2011
Author(s)
Osawa, T., Kimura, S., Terasaka, N., Inanaga, H., Suzuki, T. and Numata, T.
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Journal Title
Nature Struct Mol Biol.
Volume: 18
Pages: 1275-1280
Related Report
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[Journal Article] Taurine-containing uridine modifications in tRNA anticodons are required to decipher non-universal genetic codes inascidian2011
Author(s)
Suzuki, T., Miyauchi, K., Suzuki, T., Yokobori, S., Shigi, N., Kondow, A., Takeuchi,N., Yamagishi, A. and Watanabe, K.
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Journal Title
J. Biol. Chem.
Volume: 286
Pages: 35494-35498
Related Report
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[Journal Article] Deficit of Lys-tRNA Modification by Cdkal1Causes the Development of Type 2 Diabetes inMice2011
Author(s)
Wei, F., Suzuki, T., Watanabe, S., Kimura, S., Kaistuka, T., Fujimura, A., Matsui, H., Atta, M., Fontecave, M., Yamagata, K., Suzuki, T. and Tomizawa, K.
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Journal Title
J. Clin. Invest.
Volume: 121
Pages: 3598-608
Related Report
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[Journal Article] Actin-binding protein ABP140 is a methyltransferase responsible for3-methylcytidine at position 32 of tRNAs inSaccharomyces cerevisiae2011
Author(s)
Noma, A., Yi, S., Katoh, T., Takai, Y., Suzuki, T. and Suzuki, T.
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Journal Title
RNA
Volume: 17
Pages: 1111-1119
Related Report
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[Journal Article] Crystallization and preliminary X-ray diffraction analysis of an archaeal tRNA modification enzyme TiaS complexed with tRNA^<Ile2> and ATP2011
Author(s)
Osawa, T., Inanaga, H., Kimura, S., Terasaka, N., Suzuki, T., Numata T.
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Journal Title
Acta Crystallograph Sect F Struct Biol Cryst Commun
Volume: 67
Issue: 11
Pages: 1414-1416
DOI
Related Report
Peer Reviewed
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[Journal Article] Deficit of tRNA(Lys) modification by Cdkall causes the development of type 2 diabetes in mice2011
Author(s)
Wei FY., Suzuki T., Watanabe S., Kimura S., Kaitsuka T., Fujimura A., Matsui H., Atta M., Michiue H., Fontecave M., Yamagata K., Suzuki T., Tomizawa K.
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Journal Title
The Journal of Clinical Investigation
Volume: 121
Issue: 9
Pages: 3598-3608
DOI
Related Report
Peer Reviewed
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