Creation of Artificial Biocatalysts Capable of Catalyzing C-C Bond Formation

• Project/Area Number: 23655157
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Chemistry related to living body
• Research Institution: Okayama University
• Principal Investigator: EMA Tadashi 岡山大学, 大学院・自然科学研究科, 教授 (20263626)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 酵素反応 / 生体触媒 / 合成化学 / 不斉合成

Research Abstract

Articificial enzymes (lipases) bearing N-heterocyclic carbene (NHC) were created and used as a catalyst for the enantioselective C-C bond formation. NHC was linked to a mutated cystein residue or the active site. The articificial enzymes catalyzed the benzoin reaction with low enantioselectivity