Microantibody: Directed evolution ofMolecular-targeting Peptides in Phage-displayed Libraries
Project/Area Number |
23655161
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Chemistry related to living body
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Research Institution | Osaka Prefecture University |
Principal Investigator |
FUJII Ikuo 大阪府立大学, 大学院・理学系研究科, 教授 (70189984)
|
Co-Investigator(Kenkyū-buntansha) |
TSUMURAYA Takeshi 大阪府立大学, 大学院・理学系研究科, 准教授 (00372855)
|
Project Period (FY) |
2011 – 2012
|
Project Status |
Completed (Fiscal Year 2012)
|
Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
|
Keywords | ペプチド / 進化分子工学 / ファージライブラリー / 分子標的医薬 / 試験管内進化法 / 抗体 |
Research Abstract |
Use of antibody medicines has been limited due to the biophysical properties, immunogenicity, non-cell permeability, high cost to manufacture, and so on. To enable new applications where antibodies showsome limitations, we have developed an alternative-binding molecule with non-immunoglobulin domain. The molecule is a helix- loop- helix peptide. In previous work, we constructed a phage-displayed library of the helix-loop-helix peptides and then screened the library for G-CSF receptor to obtain a molecular-targeting peptide. Here, we examined the ability of the binding peptide as an alternative to antibody medicines. The peptide showed strong binding affinity (K_d: 4 nM) to the receptor, an enzyme-resistant property(half-life: 15 days in mouse sera), and non-immunogenicity. This peptide is named “microAntibodes” due to having the same properties as those of antibodies. Furthermore, we examined to screen the library of helix-loop-helix peptides against VEGF to successfully gain a tight-binding peptide. The semi-rational strategy, which combines directed evolution with de novodesign, provides a new way to generate a post-antibody therapy.
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Report
(3 results)
Research Products
(64 results)
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[Journal Article] An automated system for high-throughput single cell-based breeding2013
Author(s)
Nobuo Yoshimoto, Akiko Kida, Xu Jie, Masaya Kurokawa, Masumi Iijima, Tomoaki Niimi, Andrés D. Maturana, Itoshi Nikaido, Hiroki R. Ueda, Kenji Tatematsu, Katsuyuki Tanizawa, Akihiko Kondo, Ikuo Fujii, Shun'ichi Kuroda
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Journal Title
Scientific Reports
Volume: 3
Issue: 1
Pages: 1191-1191
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] PEGylated antibody in organic media2011
Author(s)
Mingzhe Liu, Muye Xu, Xian Jun Loh, Hiroshi Abe, Takeshi Tsumuraya, Ikuo Fujii, Jun Li, Tae Il Son, Yoshihiro Ito
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Journal Title
Journal of Bioscience and Bioengineering
Volume: 111
Pages: 564-568
NAID
Related Report
Peer Reviewed
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