Screening of fluorescently detectable RNA using evolutionary molecular engineering
| Project/Area Number |
23655165
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Chemistry related to living body
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
KATO Yoshio 独立行政法人産業技術総合研究所, バイオメディカル研究部門, 主任研究員 (20415657)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
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| Keywords | 核酸 / 蛍光性ＲＮＡ / 進化分子工学 / RNA / 大腸菌 / 蛍光 / 分子イメージング |
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| Research Abstract |
To obtain the spontaneously fluorescent RNA in cells, we have expressed the randomized RNA library in E. Coli and analyzed the fluorescent characteristics in E. Coli. We have constructed RNA library binding to FMN (flavin mono nucleotide), which emits fluorescence and ubiquitously dispersed all over cells, and expressed the cDNA library in E. Coli. We analyzed the fluorescent characteristics of 10000000 E.Coli clones, however, we have not obtained the expected fluorescent RNA yet.
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Report
(4 results)
Research Products
(1 results)
