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Seamless Single Cell Sorting Method Appropriately Appling Shear Flows and Distorted Electric Fields

Research Project

Project/Area Number 23656136
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Fluid engineering
Research InstitutionKyoto University

Principal Investigator

NAKABE Kazuyoshi  京都大学, 大学院・工学研究科, 教授 (80164268)

Co-Investigator(Kenkyū-buntansha) TATSUMI Kazuya  京都大学, 大学院・工学研究科, 准教授 (90372854)
Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords単細胞選別 / 流体計測 / せん断流れ / 電場強度分布 / 誘電泳動力 / 静電泳動力
Research Abstract

Numerical simulation and flow visualization on the motions of a fine spherical polystyrene particle in a microchannel installing thin membrane-type electrodes were made in order to develop an innovative method for fast and seamless sorting of single cell, in particular, red blood cell (RBC). Distorted electric fields around straight- or semi-circle- type electrodes that dielectrophoretic (DEP) forces suitably act on the particle flowing in the channel were numerically predicted. The numerically obtained trajectories of the particles show the good agreement with the visualization results, which can be expected to advance an effective manipulation and sorting of RBCs using DEP.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (33 results)

All 2013 2012 2011 Other

All Journal Article (10 results) (of which Peer Reviewed: 10 results) Presentation (17 results) (of which Invited: 1 results) Book (3 results) Remarks (3 results)

  • [Journal Article] Reaction Characteristics of Methanol Non-Catalytic Partial Oxidation Stabilized by Ceramic Honeycomb2013

    • Author(s)
      Y. Rai, H. Kogame, K. Tatsumi and K. Nakabe
    • Journal Title

      Combustion Science and Technology

      Volume: Vol. 185, No. 1 Pages: 1-16

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] 蛇行流路内における粘弾性流体流れの流れと伝熱特性2013

    • Author(s)
      巽和也,永坂亘,Heong Chee Leong,中部主敬
    • Journal Title

      日本機械学会論文集 (B 編)

      Volume: Vol. 79, No. 797 Pages: 93-103

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Development of a Numerical Model for Single Red Blood Cell Motions in Stationary Fluid in the Presence of Uniform Magnetic Field2013

    • Author(s)
      K. Tatsumi, Y. Komori, T. Arakawa, K. Nishitani and K. Nakabe
    • Journal Title

      Progress in Computational Fluid Dynamics

      Volume: Vol. 13, No. 3/4 Pages: 228-241

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] 蛇行流路内における粘弾性流体流れの流れと伝熱特性2013

    • Author(s)
      巽和也,永坂亘,Heong Chee Leong,中部主敬
    • Journal Title

      日本機械学会論文集(B編)

      Volume: 79-797 Pages: 93-103

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Numerical and Experimental Study on Measurements of Red Blood Cell Deformability in Micro-Channel Using Electric Sensors2012

    • Author(s)
      K. Tatsumi, Y. Katsumoto, R. Fujiwara, K. Nakabe
    • Journal Title

      Sensors

      Volume: Vol. 12, No. 8 Pages: 10566-10583

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] メタノール部分酸化改質反応の安定化に及ぼすセラミックハニカムの熱流動効果2012

    • Author(s)
      頼 泰弘,小亀秀行,巽和也,中部主敬
    • Journal Title

      日本機械学会論文集 (B 編)

      Volume: Vol. 78, No. 796 Pages: 2142-2152

    • NAID

      130002572590

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Numerical and Experimental Study on Measurements of Red Blood Cell Deformability in Micro-channel Using Electric Sensors2012

    • Author(s)
      K. Tatsumi, Y. Katsumoto, R. Fujiwara, K. Nakabe
    • Journal Title

      Sensors

      Volume: 12-8 Issue: 8 Pages: 10566-10583

    • DOI

      10.3390/s120810566

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] メタノール部分酸化改質反応の安定化に及ぼすセラミックハニカムの熱流動効果2012

    • Author(s)
      頼 泰弘,小亀秀行,巽和也,中部主敬
    • Journal Title

      日本機械学会論文集(B編)

      Volume: 78-796 Pages: 2142-2152

    • NAID

      130002572590

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Numerical Investigation on Fluid Flow and Heat Transfer Characteristics in a Peristaltic Micro Pump2011

    • Author(s)
      K. Tatsumi, R. Kuroki, M. Nakamura and K. Nakabe
    • Journal Title

      Progress in Computational Fluid Dynamics

      Volume: Vol. 11 Pages: 160-168

    • Related Report
      2012 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Mixing and Combustion Performance of a Baffle-Plate-Type Miniature Confined Multi-Jet2011

    • Author(s)
      K. Tatsumi, Y. Rai, Y. Niibayashi and K. Nakabe
    • Journal Title

      Journal of Fluid Science and Technology

      Volume: Vol. 6 Pages: 465-476

    • NAID

      130000797222

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Presentation] Electric Measurement, and Dielectrophoretic Manipulation to AnalyzeSingle Cell Characteristics2012

    • Author(s)
      K. Nakabe, Microchannel Flow Control
    • Organizer
      5th Conference and Exhibition of Molecular Medicine andDiagnostics
    • Place of Presentation
      Guangzhou, China
    • Related Report
      2012 Final Research Report
  • [Presentation] Flow and Heat Transfer Characteristics of Viscoelastic Fluid Flow in a Serpentine Channel - Influences of the Fluid Properties on the Performance2012

    • Author(s)
      W. Nagasaka, K. Tatsumi, O. Nakajima and K. Nakabe
    • Organizer
      Proc. 3rd Int. Forum on Heat Transfer
    • Place of Presentation
      Nagasaki, Japan
    • Related Report
      2012 Final Research Report
  • [Presentation] Heat Transfer and Reaction Characteristics of Honeycomb-Type Methanol-Fueled Reformer2012

    • Author(s)
      H. Kogame, Y. Rai, K. Tatsumi and K. Nakabe
    • Organizer
      Proc. 12th Kyoto-Seoul National-Tsinghua University Thermal Engineering Conference
    • Place of Presentation
      Kyoto, Japan
    • Related Report
      2012 Final Research Report
  • [Presentation] Flow Observation and Heat Transfer Performance of Viscoelastic Fluid Flow in a Serpentine Channel2012

    • Author(s)
      K. Tatsumi, O. Nakajima, W. Nagasaka and K. Nakabe
    • Organizer
      Proc. 7th Int. Symposium on Turbulence, Heat and Mass Transfer
    • Place of Presentation
      Sicily, Italy
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Presentation] Experimental Study on a Compact Methanol-Fueled Reformer with Heat Regeneration Using Ceramic Honeycomb (3rd Report: Observation of Reaction Structure and Its Effect on Reforming Characteristics)2012

    • Author(s)
      Y. Rai, H. Kogame, K. Tatsumi and K. Nakabe
    • Organizer
      Heat Transfer
    • Place of Presentation
      Split, Croatia
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Presentation] Detecting and Sorting of Difference in Deformability of Single Cell Flowing in a Microchannel2012

    • Author(s)
      K. Nakabe
    • Organizer
      Single Cell Analysis Congress 2012
    • Place of Presentation
      Edinburgh, United Kingdom
    • Related Report
      2012 Final Research Report
  • [Presentation] Microchannel Flow Control, Electric Measurement, and Dielectrophoretic Manipulation to Analyze Single Cell Characteristics2012

    • Author(s)
      K. Nakabe
    • Organizer
      5th Conference and Exhibition of Molecular Medicine and Diagnostics
    • Place of Presentation
      Guangzhou, China
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Flow and heat transfer characteristics of viscoelastic fluid flow in a serpentine channel - Influences of the fluid properties on the performance -2012

    • Author(s)
      W. Nagasaka, K. Tatsumi, O. Nakajima and K. Nakabe
    • Organizer
      Proc. 3rd Int. Forum on Heat Transfer
    • Place of Presentation
      Nagasaki, Japan
    • Related Report
      2012 Annual Research Report
  • [Presentation] Detecting and Sorting of Difference in Deformability of Single Cell Flowing in a Microchannel2012

    • Author(s)
      K. Nakabe
    • Organizer
      Single Cell Analysis Congress 2012(招待講演)
    • Place of Presentation
      Edinburgh, UK
    • Related Report
      2011 Research-status Report
  • [Presentation] Experimental Study on a Compact Methanol-Fueled Reformer Using Ceramic Honeycomb2011

    • Author(s)
      Y. Rai, H. Kogame, K. Tatsumi and K. Nakabe
    • Organizer
      Proc. 11th Kyoto - Seoul National - Tsinghua University Thermal Engineering Conference
    • Place of Presentation
      Jeju, Korea
    • Related Report
      2012 Final Research Report
  • [Presentation] Numerical Model for Microparticle and Lymphocyte Motions in Dieletrophoretic Manipulation Device2011

    • Author(s)
      K. Tatsumi, K. Imajou, H. Shintani, Y. Katsumoto and K. Nakabe
    • Organizer
      Proc. 15th Int. Conference on Miniaturized Systems for Chemistry and Life Sciences(microTAS - 2011)
    • Place of Presentation
      Seattle, U.S.A.
    • Related Report
      2012 Final Research Report
  • [Presentation] Numerical Modeling of Red Blood Cell Suspended in a Channel with Uniform Magnetic Field2011

    • Author(s)
      K. Tatsumi, R. Kuroki, K. Nishitani, T. Arakawa and K. Nakabe
    • Organizer
      Proc. ASME - JSME- KSME Joint Fluids Engineering Conference (AJK2011)
    • Place of Presentation
      Hamamatsu, Japan
    • Related Report
      2012 Final Research Report
  • [Presentation] Numerical Model for Microparticle and Lymphocyte Motions in Dieletrophoretic Manipulation Device2011

    • Author(s)
      Tatsumi, K. Imajou, H. Shintani, Y. Katsumoto and K. Nakabe
    • Organizer
      Proc. 15th Int. Conference on Miniaturized Systems for Chemistry and Life Sciences (microTAS-2011)
    • Place of Presentation
      Seattle, USA
    • Related Report
      2011 Research-status Report
  • [Presentation] Numerical and Experimental Study on Measurement of a Single Red Blood Cell Deformability using a Microchannel and Electric Sensors2011

    • Author(s)
      Fujiwara, K. Tatsumi, Y. Katsumoto and K. Nakabe
    • Organizer
      Proc. 3rd Int. Symposium on Computational Heat Transfer and Fluid Flow (ASCHT-2011)
    • Place of Presentation
      Kyoto, JPN
    • Related Report
      2011 Research-status Report
  • [Presentation] Motion of Red Blood Cell in Stationary Fluid under Uniform Magnetic Field2011

    • Author(s)
      Y. Komori, T. Arakawa, K. Nishitani, K. Tatsumi and K. Nakabe
    • Organizer
      Proc. 3rd Int. Symposium on Computational Heat Transfer and Fluid Flow (ASCHT-2011)
    • Place of Presentation
      Kyoto, JPN
    • Related Report
      2011 Research-status Report
  • [Presentation] Numerical Modeling of Red Blood Cell Suspended in a Channel with Uniform Magnetic Field2011

    • Author(s)
      K. Tatsumi, R. Kuroki, K. Nishitani, T. Arakawa and K. Nakabe
    • Organizer
      Proc. ASME-JSME-KSME Joint Fluids Engineering Conference (AJK2011)
    • Place of Presentation
      Hamamatsu, JPN
    • Related Report
      2011 Research-status Report
  • [Presentation] マイクロ流路内流れにおける誘電泳動現象を利用した粒子の誘導 ―櫛形電極の形状検討―2011

    • Author(s)
      新谷拓宙,今荘和也,巽和也,勝本洋一,中部主敬
    • Organizer
      日本機械学会第3回マイクロ・ナノ工学シンポジウム
    • Place of Presentation
      東京都
    • Related Report
      2011 Research-status Report
  • [Book] 伝導・対流伝熱解析のための基礎(3) , 機械の研究, 4 月号 (Vol. 64, No. 4)2012

    • Author(s)
      中部主敬
    • Publisher
      養賢堂
    • Related Report
      2012 Final Research Report
  • [Book] 伝導・対流伝熱解析のための基礎(2) , 機械の研究, 3 月号 (Vol. 64, No. 3)2012

    • Author(s)
      中部主敬
    • Publisher
      養賢堂
    • Related Report
      2012 Final Research Report
  • [Book] 伝導・対流伝熱解析のための基礎(1) , 機械の研究, 2 月号 (Vol. 64, No. 2)2012

    • Author(s)
      中部主敬
    • Publisher
      養賢堂
    • Related Report
      2012 Final Research Report
  • [Remarks] 京都大学大学院工学研究科機械理工学専攻熱材料力学研究室

    • URL

      http://mtfm.me.kyoto-u.ac.jp/

    • Related Report
      2012 Final Research Report
  • [Remarks] 京都大学大学院工学研究科機械理工学専攻熱材料力学研究室

    • URL

      http://mtfm.me.kyoto-u.ac.jp/

    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://mtfm.me.kyoto-u.ac.jp/

    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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