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Development of electroinjection method for loading single fish egg with cryo- and lyoprotective agents

Research Project

Project/Area Number 23656144
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Thermal engineering
Research InstitutionThe University of Tokyo

Principal Investigator

SHIRAKASHI Ryo  東京大学, 生産技術研究所, 准教授 (80292754)

Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords魚卵 / 誘電特性 / 物質輸送 / 誘電操作 / 細胞操作 / 膜輸送
Research Abstract

Cryo- or lyopreservation of fish egg for seedling production has not been succeeded yet mainly because the method of loading eggs with mass cryo-/lyoprotective agents still not exist so far. In this study, a high performance motionless device for needling a fish egg that utilizes the electro manipulation has been developed. In addition, the basic electro manipulation parameters for optimizing the needling have been achieved experimentally.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (13 results)

All 2013 2012

All Journal Article (4 results) (of which Peer Reviewed: 2 results) Presentation (8 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] 魚卵の誘電特性の測定2013

    • Author(s)
      白樫了,Vladimir Sukhorukov
    • Journal Title

      第25回バイオエンジニアリング講演会講演論文集

      Volume: No.12-48 Pages: 179-180

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Journal Article] Sukhorukov, Changes in the dielectric properties of medaka fish embryos during development, studied by electrorotation2012

    • Author(s)
      Ryo Shirakashi,Miriam Mischke,Peter Fischer, Simon Memmel,Georg Krohne,Gunter R.Fuhr, Heiko Zimmermann,Vladimir L.
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: Vol.428 Pages: 127-131

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] 魚卵内への高効率物質輸送法2012

    • Author(s)
      白樫了,安井龍生
    • Journal Title

      熱工学コンファレンス講演論文集

      Volume: No.12-62 Pages: 365-366

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Journal Article] Changes in the dielectric properties of medaka fish embryos during development, studied by electrorotation2012

    • Author(s)
      Ryo Shirakashi, Miriam Mischke, Peter Fischer, Simon Memmel, Georg Krohne, Günter R. Fuhr, Heiko Zimmermann,Vladimir L. Sukhorukov
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 428 Issue: 1 Pages: 127-131

    • DOI

      10.1016/j.bbrc.2012.10.019

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Presentation] 魚卵の誘 電特性の測定2013

    • Author(s)
      白樫了,Vladimir Sukhorukov
    • Organizer
      日本機械学会第25回 バ イオエンジニアリング講演会
    • Place of Presentation
      産業技術総合技術研究所つくばセンタ-
    • Year and Date
      2013-01-09
    • Related Report
      2012 Final Research Report
  • [Presentation] 魚卵の誘電特性の測定2013

    • Author(s)
      白樫 了,Vladimir Sukhorukov
    • Organizer
      日本機械学会 第25回バイオエンジニアリング講演会
    • Place of Presentation
      産業技術総合技術センター
    • Related Report
      2012 Annual Research Report
  • [Presentation] 魚卵内への高効率 物質輸送法2012

    • Author(s)
      白樫了,安井龍生
    • Organizer
      日本機械学会熱工学コン ファレンス
    • Place of Presentation
      熊本大学
    • Year and Date
      2012-11-18
    • Related Report
      2012 Final Research Report
  • [Presentation] Changes in the dielectric properties of medaka fish embryos during development studied by electrorotation2012

    • Author(s)
      V.L.Sukhorukov, R.Shirakashi
    • Organizer
      Broadband Dielectric Spectroscopy and its Applications (BDS2012)
    • Place of Presentation
      ライプチヒ大学ドイツ
    • Year and Date
      2012-09-03
    • Related Report
      2012 Final Research Report
  • [Presentation] Dielectric spectroscopy of medaka fish embryos using Electrorotation2012

    • Author(s)
      R.Shirakashi, V.L.Sukhorukov
    • Organizer
      Broadband Dielectric Spectroscopy and its Applications(BDS2012)
    • Place of Presentation
      ライプチヒ大学ドイツ
    • Year and Date
      2012-09-03
    • Related Report
      2012 Final Research Report
  • [Presentation] Changes in the dielectric properties of medaka fish embryos during development studied by electrorotation2012

    • Author(s)
      V.L. Sukhorukov, R. Shirakashi
    • Organizer
      Broadband Dielectric Spectroscopy and its Applications (BDS2012)
    • Place of Presentation
      ライプチヒ大学(ドイツ)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Dielectric spectroscopy of medaka fish embryos using Electrorotation2012

    • Author(s)
      R. Shirakashi, V.L. Sukhorukov
    • Organizer
      Broadband Dielectric Spectroscopy and its Applications (BDS2012)
    • Place of Presentation
      ライプチヒ大学(ドイツ)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 魚卵内への高効率物質輸送法2012

    • Author(s)
      白樫 了,安井龍生
    • Organizer
      日本機械学会 熱工学コンファレンス
    • Place of Presentation
      熊本大学
    • Related Report
      2012 Annual Research Report
  • [Patent(Industrial Property Rights)] 穿針装置および穿針方法2013

    • Inventor(s)
      白樫 了,安井龍生
    • Industrial Property Rights Holder
      東京大学
    • Industrial Property Rights Type
      特許
    • Filing Date
      2013-03-29
    • Related Report
      2012 Annual Research Report

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Published: 2011-08-05   Modified: 2019-07-29  

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