Development of highly-efficient process for producing giant unilamellar vesicles (GUV) by controlling microscopic flow field

• Project/Area Number: 23656145
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Thermal engineering
• Research Institution: The University of Tokyo
• Principal Investigator: TAKANO Kiyoshi 東京大学, 生産技術研究所, 助教 (60302626)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: リポソーム / 振動流 / 生体熱工学 / マイクロ流動場

Research Abstract

We have found that the growth rate of liposomes produced by rehydrating a dried phospholipid layer on a silicone substrate is enhanced by applying the oscillating flow of specific frequency. It has also been found that the initial number of budding liposome from dried phospholipid layer is very sensitive to the electric conductivity of rehydrating solution, although the fluid flow around the initial budding liposome enhances the growing of liposome.