A CHARENGE FOR CRYOPRESERVATION OF FISH EGGS WITH LIQUID MENISCUS
| Project/Area Number |
23656154
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Thermal engineering
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| Research Institution | Kyushu Institute of Technology |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | 凍結保存 / 魚卵 / 液体メニスカス / 脱水冷凍 / メニスカスホルダー / 冷凍保存 / 細胞膜損傷 |
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| Research Abstract |
In order to reduce mechanical stress induced by external ice formation in a freezing of fish egg, we suggest a new method using liquid meniscus. We have developed the meniscus holder that holds the liquid-meniscus up to the top of the fish egg. The membrane of the fish egg is coated by the thin film of torehalose aqueous and can be protected from the external ice formation. Also, we used the dehydro-freezing in order to reduce the rupture due to the internal ice formation. The experimental results show that the fish eggs with meniscus holder have a high shape-preservation rate after the thawing.
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Report
(3 results)
Research Products
(4 results)
