| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Research Abstract |
Oligotrophic phenol-utilizing bacterium, Cupriavidus sp. TW2, is able to degrade Trichloroethene (TCE) by phenol hydroxylase (PH) induced by phenol. Homologous recombination was carried out to insert the tac promoter (Ptac) upstream of PH genes for constitutive expression. The resultant strain, TW2-P, showed the higher TCE degradability without the phenol induction.
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