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Development of processing techniques for liposomes using membranes

Research Project

Project/Area Number 23656496
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Properties in chemical engineering process/Transfer operation/Unit operation
Research InstitutionKogakuin University

Principal Investigator

NAKAO Shin-ichi  工学院大学, 工学部, 教授 (00155665)

Co-Investigator(Kenkyū-buntansha) AKAMATSU Kazuki  工学院大学, 工学部, 助教 (50451795)
Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywordsリポソーム / SPG 膜 / 脂質 / 単分散 / 流動性 / アルコール / SPG膜 / DDS
Research Abstract

In this study, we developed a facile and novel method for preparing liposomes simply by permeating lipid-alcohol solutions through Shirasu porous glass (SPG) membranes.This is based on the phenomenon in which an interface between a lipid-alcohol solution and water is formed and the two solutions mix with each other at the surface of an SPG membrane when the lipid-alcohol solution is slowly permeated through the membrane; this reduces the concentration of the alcohol solution and induces spontaneous formation of liposomes. We successfully prepared 100-nm monodispersed liposomes by this method using lipid-isopropyl alcohol (IPA) and lipid-ethanol (EtOH) solutions. Additionally, an increase in the liposome size was clearly observed in freezing-thawing treatment when IPA was used as the alcohol, whereas the liposome size was unchanged by freezing-thawing treatment when EtOH was used. It is interesting that the alcohol affected the liposome properties, in particular the membrane fluidity, even although no effect of the alcohol on the liposome structure, such as size, monodispersity, was observed.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (4 results)

All 2013 Other

All Presentation (3 results) Remarks (1 results)

  • [Presentation] SPG 膜を利用したリポソーム調製法の開発2013

    • Author(s)
      赤松憲樹,清水勇輔,清水龍太郎,中尾真一
    • Organizer
      日本膜学会第35 年会
    • Place of Presentation
      早稲田大学
    • Related Report
      2012 Final Research Report
  • [Presentation] SPG 膜を用いたリポソーム調製法に関する研究2013

    • Author(s)
      清水勇輔,清水龍太郎,赤松憲樹,中尾真一
    • Organizer
      日本海水学会若手会第4回学生研究発表会
    • Place of Presentation
      横浜国立大学
    • Related Report
      2012 Final Research Report
  • [Presentation] SPG膜を用いたリポソーム調製法に関する研究2013

    • Author(s)
      清水勇輔、清水龍太郎、赤松憲樹、中尾真一
    • Organizer
      日本海水学会若手会第4回学生研究発表会
    • Place of Presentation
      横浜国立大学
    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://www.ns.kogakuin.ac.jp/~wwb1051/index.html

    • Related Report
      2012 Final Research Report

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Published: 2011-08-05   Modified: 2019-07-29  

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