Preparation of microsphere with minute through-holes by using multi emulsion and its application
Project/Area Number |
23656498
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Research Category |
Grant-in-Aid for Challenging Exploratory Research
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Allocation Type | Multi-year Fund |
Research Field |
Properties in chemical engineering process/Transfer operation/Unit operation
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Research Institution | Sojo University |
Principal Investigator |
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Project Period (FY) |
2011-04-28 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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Keywords | 微粒子 / 貫通型細孔 / マルチエマルション / 固定化酵素 / 糖 / マイクロリアクター / 薄膜 / 接着 / 固体化酵素 |
Outline of Final Research Achievements |
In this study, I proposed a simpler preparation method for microsphere with minute through-holes by lyophilization of O/W/O multiple emulsion. And I succeeded in immobilizing of enzyme in PEG microsphere with minute through-holes. The effects of sugar addition on enzymatic activity of the immmobilized enzyme were investigated and the optimum concentrations of sugars were found. Furthermore, it was found that immobilized enzyme in PEG microsphere with minute-holes, which adhered to the surface of microchannel by simple method, exhibited high activity.
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Report
(5 results)
Research Products
(3 results)