Preparation of microsphere with minute through-holes by using multi emulsion and its application

Research Project

Project/Area Number	23656498
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Multi-year Fund
Research Field	Properties in chemical engineering process/Transfer operation/Unit operation
Research Institution	Sojo University
Principal Investigator	SAKOGUCHI Akihiro 崇城大学, 工学部, 教授 (30196141)
Project Period (FY)	2011-04-28 – 2015-03-31
Project Status	Completed (Fiscal Year 2014)
Budget Amount *help	¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000) Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000) Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000) Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywords	微粒子 / 貫通型細孔 / マルチエマルション / 固定化酵素 / 糖 / マイクロリアクター / 薄膜 / 接着 / 固体化酵素
Outline of Final Research Achievements	In this study, I proposed a simpler preparation method for microsphere with minute through-holes by lyophilization of O/W/O multiple emulsion. And I succeeded in immobilizing of enzyme in PEG microsphere with minute through-holes. The effects of sugar addition on enzymatic activity of the immmobilized enzyme were investigated and the optimum concentrations of sugars were found. Furthermore, it was found that immobilized enzyme in PEG microsphere with minute-holes, which adhered to the surface of microchannel by simple method, exhibited high activity.

Report

(5 results)