Screening of protein-protein interactions based on a growth signal of mammalian cells
| Project/Area Number |
23656516
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 蛋白質 / 細胞・組織 / シグナル伝達 / 生体機能利用 / プロテオーム |
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| Research Abstract |
In this study, we aimed to develop a method to easily detect protein-protein interactions in live mammalian cells. Instead of conventional methods employing fluorescence or luminescence as a read-out, we developed a novel method to detect protein-protein interactions based on cell growth. Using known interacting protein pairs, we successfully induced cell growth dependent on the protein-protein interactions.
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Report
(3 results)
Research Products
(52 results)
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[Journal Article] Growth promotion ofgenetically modified hematopoieticprogenitors using an antibody/c-Mpl chimera2011
Author(s)
Kawahara, M., Chen, J., Sogo, T., Teng, J., Otsu, M., Onodera, M., Nakauchi, H., Ueda, H. and Nagamune, T.
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Journal Title
Cytokine
Volume: 55
Issue: 3
Pages: 402-408
DOI
Related Report
Peer Reviewed
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