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Construction of versatile fluoroimmunoassay probe based on quench-release principle

Research Project

Project/Area Number 23656517
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Biofunction/Bioprocess
Research InstitutionTokyo Institute of Technology (2012)
The University of Tokyo (2011)

Principal Investigator

UEDA Hiroshi  東京工業大学, 資源化学研究所, 教授 (60232758)

Co-Investigator(Kenkyū-buntansha) OHASHI Hiroyuki  東京大学, 特任研究員 (40589454)
Co-Investigator(Renkei-kenkyūsha) OHMURO Yuki (MATSUYAMA Yuki)  東京大学, 特任研究員 (30571088)
Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsバイオセンサー / 生物・生体工学 / 分析科学 / 蛋白質 / 可視化 / 抗体 / 表面プラズモン共鳴法 / 蛍光 / タンパク質間相互作用
Research Abstract

With the aim of extending Quenchbody principle that can detect various antigens in a rapid and sensitive manner, an antibody binding protein probe that is fluorescence labeled at its terminus was constructed. When this probe was mixed with an anti-vimentin human Fab fragment, the dye of the probe was quenched by the Trp residues in the antibody. Moreover, when the antigen vimentin was added, dose-dependent release of the quenching was observed, thus resulted in successful detection of antigen by fluorescence increase.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (42 results)

All 2013 2012 2011 Other

All Journal Article (16 results) (of which Peer Reviewed: 16 results) Presentation (19 results) (of which Invited: 2 results) Book (2 results) Remarks (3 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] Demonstration of protein-fragment complementation assay using purified firefly luciferase fragments2013

    • Author(s)
      Y. Ohmuro-Matsuyama, C.I. Chung, and H. Ueda
    • Journal Title

      BMC Biotechnol

      Volume: 13 Pages: 31-31

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Detection of vimentin serine phosphorylation by multicolor Quenchbodies2013

    • Author(s)
      H.-J. Jeong, Y. Ohmuro-Matsuyama, H. Ohashi, F. Ohsawa, Y. Tatsu, M. Inagaki, and H. Ueda
    • Journal Title

      Biosens. Bioelectron

      Volume: 40 Pages: 17-23

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Isolation of recombinant phage antibodies targeting the hemagglutinin cleavage site of highly pathogenic avian influenza virus2013

    • Author(s)
      J. Dong, A. Sakurai, N. Nomura, E.Y. Park, F. Shibasaki, and H. Ueda
    • Journal Title

      PLOS ONE

      Volume: 8

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Detection of vimentin serine phosphorylation by multicolor Quenchbodies2013

    • Author(s)
      Jeong, H.J.
    • Journal Title

      Biosensors and Bioelectronics

      Volume: 40 Issue: 1 Pages: 17-23

    • DOI

      10.1016/j.bios.2012.06.030

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Demonstration of protein-fragment complementation assay using purified firefly luciferase fragments2013

    • Author(s)
      Ohmuro-Matsuyama, Y.
    • Journal Title

      BMC Biotechnology

      Volume: 13 Issue: 1 Pages: 31-31

    • DOI

      10.1186/1472-6750-13-31

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Isolation of recombinant phage antibodies targeting the hemagglutinin cleavage site of highly pathogenic avian influenza virus2013

    • Author(s)
      Jinhua Dong, Akira Sakurai, Namiko Nomura, Enoch Y.Park, Futoshi Shibasaki, Hiroshi Ueda
    • Journal Title

      PLoS One

      Volume: 8巻 Issue: 4 Pages: e61158-e61158

    • DOI

      10.1371/journal.pone.0061158

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Open-sandwich molecular imprinting: making a recognition matrix with antigen-imprintedantibody fragments2012

    • Author(s)
      K. Minami, M. Ihara, S. Kuroda, H. Tsuzuki, and H. Ueda
    • Journal Title

      Bioconj. Chem

      Volume: 23 Pages: 1463-1469

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Improved detection sensitivity and selectivity attained by Open-Sandwich selection of ananti-estradiol antibody2012

    • Author(s)
      X. Liu, M. Eichenberger, Y. Fujioka, J. Dong, and H. Ueda
    • Journal Title

      Anal. Sci

      Volume: 28 Pages: 861-867

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Detection of small molecule diagnostic markers with phage-based Open-Sandwich Immuno-PCR2012

    • Author(s)
      J. Dong, S. Hasan, Y. Fujioka, and H. Ueda
    • Journal Title

      J. Immunol.Methods

      Volume: 377 Pages: 1-7

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Open-sandwich molecular imprinting: making a recognition matrix with antigen-imprinted antibody fragments2012

    • Author(s)
      Minami, K.
    • Journal Title

      Bioconjugate Chemistry

      Volume: 23 Issue: 7 Pages: 1463-1469

    • DOI

      10.1021/bc3000782

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of an Electric Charge-Tunable Micro-column for Capillary Liquid Chromatography2012

    • Author(s)
      Liu, X.
    • Journal Title

      Analytical Sciences

      Volume: 28 Issue: 9 Pages: 853-859

    • DOI

      10.2116/analsci.28.853

    • NAID

      130004442134

    • ISSN
      0910-6340, 1348-2246
    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Detection of small molecule diagnostic markers with phage-based Open-Sandwich Immuno-PCR2012

    • Author(s)
      J.Dong, S.Hasan, Y.Fujioka, H.Ueda
    • Journal Title

      J.Immunol.Methods

      Volume: 377 Issue: 1-2 Pages: 1-7

    • DOI

      10.1016/j.jim.2012.01.005

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Activation of circularly permutatedβ-lactamase tethered to antibody domainsby specific small molecules2011

    • Author(s)
      M. Kojima, H. Iwai, J. Dong, SL. Lim, S. Ito, K. Okumura, M. Ihara, and H. Ueda
    • Journal Title

      Bioconj. Chem

      Volume: 22 Pages: 633-641

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Quenchbodies" : quench-based antibody probes that show antigen-dependent fluorescence2011

    • Author(s)
      R. Abe, H. Ohashi, I. Iijima, M.Ihara, H. Takagi, T. Hohsaka, and H. Ueda
    • Journal Title

      J. Am. Chem. Soc

      Volume: 133 Pages: 17386-17394

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] "Quenchbodies" : quench-based antibody probes that show antigen-dependent fluorescence2011

    • Author(s)
      Ryoji Abe, Hiroyuki Ohashi, Issei Iijima, Masaki Ihara, Hiroaki Takagi, Takahiro Hohsaka, Hiroshi Ueda
    • Journal Title

      J.Am.Chem.Soc.

      Volume: 133 Issue: 43 Pages: 17386-17394

    • DOI

      10.1021/ja205925j

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Activation of circularly permutated β-lactamase tethered to antibody domains by specific small molecules2011

    • Author(s)
      M.Kojima*, H.Iwai*, J.Dong, S.-L.Lim, S.Ito, K.Okumura, M.Ihara, H.Ueda *equal
    • Journal Title

      Bioconj.Chem.

      Volume: 22 Issue: 4 Pages: 633-641

    • DOI

      10.1021/bc1004125

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] 高親和性抗体結合タンパク質を利用した蛍光免疫センサー構築法の開発2013

    • Author(s)
      児島智樹, 大橋広行, 上田宏
    • Organizer
      化学工学会第78年会
    • Place of Presentation
      大阪
    • Year and Date
      2013-03-19
    • Related Report
      2012 Final Research Report
  • [Presentation] 高親和性抗体結合タンパク質を利用した蛍光免疫センサー構築法の開発2013

    • Author(s)
      児島 智樹
    • Organizer
      化学工学会 第78年会
    • Place of Presentation
      大阪
    • Related Report
      2012 Annual Research Report
  • [Presentation] 抗原結合により光る蛍光標識抗体Quenchbody(Q-body)の開発2012

    • Author(s)
      上田宏
    • Organizer
      第3回日本分子生物学会年会
    • Place of Presentation
      福岡
    • Year and Date
      2012-12-14
    • Related Report
      2012 Final Research Report
  • [Presentation] 抗体結合タンパク質を利用した蛍光免疫センサー構築法の検討2012

    • Author(s)
      児島智樹, 大橋広行, 上田宏
    • Organizer
      化学工学会第44回秋季大会
    • Place of Presentation
      仙台
    • Year and Date
      2012-09-19
    • Related Report
      2012 Final Research Report
  • [Presentation] 新規蛍光免疫素子Quenchbodyによる各種バイオマーカーの迅速高感度検出2012

    • Author(s)
      上田宏
    • Organizer
      日本プロテオーム学会2012年大会
    • Place of Presentation
      東京
    • Year and Date
      2012-07-27
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Presentation] "Quenchbodies" :quench-based antibody probes that fluoresce upon antigen binding2012

    • Author(s)
      Ueda, Hiroshi
    • Organizer
      Biosensors 2012
    • Place of Presentation
      Cancun, Mexico
    • Year and Date
      2012-05-16
    • Related Report
      2012 Final Research Report
  • [Presentation] 抗原結合により光る蛍光標識抗体Quenchbody (Q-body)の開発2012

    • Author(s)
      上田 宏
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] ”Quenchbodies” : quench-based antibody probes that fluoresce upon antigen binding2012

    • Author(s)
      Ueda, Hiroshi
    • Organizer
      Biosensors 2012
    • Place of Presentation
      Cancun, Mexico
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] 抗体結合タンパク質を利用した蛍光免疫センサー構築法の検討2012

    • Author(s)
      児島 智樹
    • Organizer
      化学工学会 第44回秋季大会
    • Place of Presentation
      仙台
    • Related Report
      2012 Annual Research Report
  • [Presentation] Detection of vimentin serine phosphorylation by Quenchbodies2011

    • Author(s)
      Hee-Jin Jeong, Hiroyuki Ohashi, Yuki Ohmuro- Matsuyama Masaki Inagaki and Hiroshi Ueda
    • Organizer
      AsiaSense 2011
    • URL

      http://www.asiasense2011.org/

    • Place of Presentation
      済州,韓国
    • Year and Date
      2011-10-26
    • Related Report
      2012 Final Research Report
  • [Presentation] 蛍光標識抗体Quenchbodyを利用した麻薬・覚醒剤類の迅速高感度検出,蛍光標識抗体Quenchbodyを利用した麻薬・覚醒剤類の迅速高感度検2011

    • Author(s)
      大橋広行・阿部亮二・高木広明・上田宏
    • Place of Presentation
      名古屋
    • Year and Date
      2011-09-15
    • Related Report
      2012 Final Research Report
  • [Presentation] オープンサンドイッチ原理と新規相互作用検出系による蛋白質リン酸化修飾の検出2011

    • Author(s)
      大室(松山)有紀,北岡優一,ジョンヒジン,達吉郎,稲垣昌樹,上田宏
    • Organizer
      第11回蛋白質科学会年会
    • Place of Presentation
      大阪
    • Year and Date
      2011-06-08
    • Related Report
      2012 Final Research Report
  • [Presentation] 非天然アミノ酸導入法による蛍光免疫センサー蛋白質Quenchbodyの創製2011

    • Author(s)
      上田宏
    • Organizer
      第11回蛋白質科学会年会1WC-2
    • Place of Presentation
      大阪
    • Year and Date
      2011-06-07
    • Related Report
      2012 Final Research Report
  • [Presentation] Quenchbodies : Antibody Probes That Fluoresce Upon Antigen Binding (Selected as oral presentation)2011

    • Author(s)
      Hiroshi Ueda, Ryoji Abe, HiroakiTakagi, and Takahiro Hohsaka
    • Organizer
      Quenchbodies : Antibody Probes That Fluoresce Upon Antigen Binding (Selected as oral presentation)
    • Place of Presentation
      上海,中国
    • Year and Date
      2011-05-12
    • Related Report
      2012 Final Research Report
  • [Presentation] Detection of vimentin serine phosphorylation by Quenchbodies2011

    • Author(s)
      Hee-Jin Jeong, Hiroyuki Ohashi, Yuki Ohmuro-Matsuyama, Masaki Inagaki and Hiroshi Ueda
    • Organizer
      AsiaSense 2011 (http://www.asiasense2011.org/)
    • Place of Presentation
      済州, 韓国
    • Related Report
      2011 Research-status Report
  • [Presentation] 非天然アミノ酸導入法による蛍光免疫センサー蛋白質Quenchbodyの創製2011

    • Author(s)
      上田 宏
    • Organizer
      第11回蛋白質科学会年会 1WC-2(招待講演)
    • Place of Presentation
      ホテル阪急エキスポパーク
    • Related Report
      2011 Research-status Report
  • [Presentation] オープンサンドイッチ原理と新規相互作用検出系による蛋白質リン酸化修飾の検出2011

    • Author(s)
      大室(松山)有紀,北岡 優一,ジョン ヒジン,達 吉郎,稲垣 昌樹,上田 宏
    • Organizer
      第11回蛋白質科学会年会 2WB-3(招待講演)
    • Place of Presentation
      ホテル阪急エキスポパーク
    • Related Report
      2011 Research-status Report
  • [Presentation] Quenchbodies : Antibody Probes That Fluoresce Upon Antigen Binding (Selected as oral presentation)2011

    • Author(s)
      °Hiroshi Ueda, Ryoji Abe, Hiroaki Takagi, and Takahiro Hohsaka
    • Organizer
      ACB (Asian Congress of Biotechnology) 2011
    • Place of Presentation
      上海,中国
    • Related Report
      2011 Research-status Report
  • [Presentation] 蛍光標識抗体Quenchbodyを利用した麻薬・覚醒剤類の迅速高感度検出2011

    • Author(s)
      ○大橋 広行・阿部 亮二 ・高木 広明・上田 宏
    • Organizer
      化学工学会第43回秋季大会
    • Place of Presentation
      名古屋工業大学
    • Related Report
      2011 Research-status Report
  • [Book] -人と医学のステージへ拡大する細胞周期2013

    • Author(s)
      上田宏, ジョンヒジン
    • Publisher
      羊土社
    • Related Report
      2012 Final Research Report
  • [Book] 実験医学増刊―人と医学のステージへ拡大する細胞周期2013 中山敬一編2013

    • Author(s)
      上田 宏
    • Publisher
      羊土社
    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://www.ueda.res.titech.ac.jp/index.html

    • Related Report
      2012 Final Research Report
  • [Remarks] 上田研究室

    • URL

      http://www.ueda.res.titech.ac.jp/index.html

    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://park.itc.u-tokyo.ac.jp/hueda/

    • Related Report
      2011 Research-status Report
  • [Patent(Industrial Property Rights)] 抗体結合タンパク質2013

    • Inventor(s)
      上田宏, 大橋広行, 阿部亮二
    • Industrial Property Rights Holder
      東京大学,ウシオ電機
    • Filing Date
      2013-02-15
    • Related Report
      2012 Final Research Report
  • [Patent(Industrial Property Rights)] 抗体結合タンパク質2013

    • Inventor(s)
      上田 宏, 大橋 広行,阿部 亮二
    • Industrial Property Rights Holder
      東京大学,ウシオ電機
    • Industrial Property Rights Type
      特許
    • Filing Date
      2013-02-15
    • Related Report
      2012 Annual Research Report

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Published: 2011-08-05   Modified: 2019-09-09  

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