| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
|---|
| Research Abstract |
The labeling method consisting of a peptide tag and a fluorescent dye which can specifically label a target protein and turn on the fluorescence after labeling was developed. We found the pair of tag sequences and a dye which fluorescent intensities were increased 2.6 times after specific labeling via soltase-mediated ligation. Furthermore, to enable intracellular labeling, a method for intracellular specific ligation was successfully developed by transducing Streptococcus pyogenes sortase A into living mammalian cells.
|
