Measurement of mechanical properties of the cell nucleus for an exhaustive evaluation of stem cell differentiation
| Project/Area Number |
23656520
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Tokyo University of Agriculture and Technology |
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Principal Investigator |
MATUNAGA Tadashi 東京農工大学, 大学院・工学研究院, 学長 (10134834)
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| Co-Investigator(Kenkyū-buntansha) |
YOSHINO Tomoko 東京農工大学, 大学院・工学研究院, 准教授 (30409750)
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| Project Period (FY) |
2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2011: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
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| Keywords | バイオセパレーション / 細胞・組織 / マイクロ・ナノデバイス |
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| Research Abstract |
In this study, the microcavity array which enables entrapment of 10, 000 single cells with high efficiency was utilized for the quantitative evaluation of cellular deformability. A quantitative measurement procedure of the three dimensional image of the cells trapped on the microcavity array by image analysis using the confocal microscopy was established. Using these techniques, cellular deformability of normal cells and cancer cells were quantitatively evaluated for considering the correlation between their deformability and progress degrees of differentiation or canceration.
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Report
(2 results)
Research Products
(6 results)
