Challenge to microbial synthesis of block copolyesters by regulated gene expression

Research Project

Project/Area Number	23656521
Research Category	Grant-in-Aid for Challenging Exploratory Research
Allocation Type	Multi-year Fund
Research Field	Biofunction/Bioprocess
Research Institution	Tokyo Institute of Technology
Principal Investigator	FUKUI Toshiaki 東京工業大学, 大学院・生命理工学研究科, 准教授 (80271542)
Project Period (FY)	2011 – 2012
Project Status	Completed (Fiscal Year 2012)
Budget Amount *help	¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000) Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000) Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords	生物機能工学 / 生分解性プラスチック / 合成生物学 / 微生物 / ポリヒドロキシアルカン酸 / 発現制御
Research Abstract	This study aimed at microbial synthesis of polyhydroxyalkanoate (PHA) copolymers in which two kinds of monomers were highly block-covalently bonded by regulated expression of the biosynthetic genes. Firstly, copolymer biosynthesis pathways suitable for regulatory gene expression were established in Escherichia coli. Then, recombinant strains of E. coli equipped with a regulatory PHA biosynthesis pathways were constructed, and PHA biosynthesis by the strains and structural analyses of accumulated PHAs are nowunder progress.

Report

(3 results)