A study of the enucleation of embryonic stem cells and the effective induction of the pluripotent stem cells by the use of the enucleated embryonic stem cells

• Project/Area Number: 23656524
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biofunction/Bioprocess
• Research Institution: Kyoto University
• Principal Investigator: KAWAMOTO Takuo 京都大学, 放射性同位元素総合センター, 教授 (10231276)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
• Keywords: ES細胞 / 多能性幹細胞 / iPS細胞 / 初期化 / ＥＳ細胞

Research Abstract

World-wide attention has been focused on the development of the efficient methods for induction of safe iPS cells. Therefore, the method to obtain the pure cytoplast prepared from the enucleated ES cells and karyoplast prepared from somatic cells was at first examined in order to develop the efficient methods for induction of the pluripotent stem cells by the use of the enucleated ES cells. As a result, it was found that the combination of the density gradient centrifugation method using Ficoll and the treatment with cytochalasin B was promising.