Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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Research Abstract |
World-wide attention has been focused on the development of the efficient methods for induction of safe iPS cells. Therefore, the method to obtain the pure cytoplast prepared from the enucleated ES cells and karyoplast prepared from somatic cells was at first examined in order to develop the efficient methods for induction of the pluripotent stem cells by the use of the enucleated ES cells. As a result, it was found that the combination of the density gradient centrifugation method using Ficoll and the treatment with cytochalasin B was promising.
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