• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Developmentofcontrol for amyloid toxicity by novel Screening system

Research Project

Project/Area Number 23656525
Research Category

Grant-in-Aid for Challenging Exploratory Research

Allocation TypeMulti-year Fund
Research Field Biofunction/Bioprocess
Research InstitutionOkayama University (2012)
Osaka University (2011)

Principal Investigator

SHIMANOUCHI Toshinori  岡山大学, 大学院・環境生命科学研究科, 准教授 (10335383)

Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsメンブレン・ストレスバイオテクノロジー / LIPOzyme / 生体膜 / 人工酵素 / バイオリアクター / メンブレノーム / アミロイド / 細胞毒性
Research Abstract

Amyloid fibrils is generally propagated on biomembranes to induce the cell toxicity. Meanwhile, there is no methodology to stop the aforesaid event. In this study, we challenged the development of toxicity control of amyloid fibrils using liposomes. It has been demonstrated that liposome library could give (i) the adequate liposome with lipid composition advantageous for a control of toxicity of amyloid fibrils and (ii) LIPOzyme with the function to promote the inhibitory of fibril formation or disaggregatiojn of fibrils. Those results have implied a possibility on the development of detoxic process of amyloid fibrils by using liposomes.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Research-status Report
  • Research Products

    (43 results)

All 2013 2012 2011

All Journal Article (25 results) (of which Peer Reviewed: 23 results) Presentation (18 results)

  • [Journal Article] Membrane fusion mediated by phospholipase C under endosomal pH conditions2013

    • Author(s)
      Toshinori Shimanouchi, Hidenori Kawasaki, Makoto Fuse, Hiroshi Umakoshi, Ryoichi Kuboi
    • Journal Title

      Coll. Surf.B

      Volume: 103 Pages: 75-83

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Enhanced Cytotoxicity for Colon 26 Cells Using Doxorubicin-Loaded Sorbitan Monooleate (Span 80) Vesicles2013

    • Author(s)
      Keita Hayashi, Tsuyoshi Tatsui, ToshinoriShimanouchi, Hiroshi Umakoshi
    • Journal Title

      Int'l. J. Biol. Sci.

      Volume: 9 Pages: 142-8

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Effect of Liposome Membranes against Disaggregation of Amyloid βFibrils by Dopamine2013

    • Author(s)
      Vu Thi Huong, Toshinori Shimanouchi, Daisuke Ishikawa, Tadaharu Matsumoto, Hisashi Yagi, Yuji Goto, Hiroshi Umakoshi, Ryoichi Kuboi
    • Journal Title

      Biochem. Eng. J.

      Volume: 71 Pages: 118-26

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Growth Behavior of AβProtofibrils on Liposome Membranes and Their Membrane Perturbation Effect2013

    • Author(s)
      Toshinori Shimanouchi, Keiichi Nishiyama,Azusa Hiroiwa, Huong Thi Vu, Nachi Kitaura, Hiroshi Umakoshi, Ryoichi Kuboi
    • Journal Title

      Biochem. Eng. J.

      Volume: 71 Pages: 81-8

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Membrane fusion mediated by phospholipase C under endosomal pH conditions2013

    • Author(s)
      Toshinori Shimanouchi, Hidenori Kawasaki, Makoto Fuse, Hiroshi Umakoshi, Ryoichi Kuboi
    • Journal Title

      Coll. Surf. B

      Volume: 103 Pages: 75-83

    • DOI

      10.1016/j.colsurfb.2012.10.011

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Enhanced cytotoxicity for colon 26 cells using Doxorubicin-loaded sorbitan monooleate(span 80) vesicles2013

    • Author(s)
      林啓太
    • Journal Title

      International journal of biological sciences

      Volume: 9(2) Issue: 2 Pages: 142-148

    • DOI

      10.7150/ijbs.5453

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Growth Behavior of Aβ Protofibrils on Liposome Membranes and Their Membrane Perturbation Effect2013

    • Author(s)
      Toshinori Shimanouchi, Keiichi Nishiyama, Azusa Hiroiwa, Huong Thi Vu, Nachi Kitaura, Hiroshi Umakoshi, Ryoichi Kuboi
    • Journal Title

      Biochem. Eng. J.

      Volume: 71 Pages: 81-88

    • DOI

      10.1016/j.bej.2012.11.006

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effect of Copper (II) Ion against Elongation Behavior of Amyloid βFibrils on Liposome Membranes2012

    • Author(s)
      Toshinori Shimanouchi , R.Onishi, N. Kitaura, H.Umakoshi, R.Kuboi
    • Journal Title

      Cryst. Res. Tech.

      Volume: 47 Pages: 101-8

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Secondary Nucleation of Amyloid Fibrils on Liposome Membranes2012

    • Author(s)
      Toshinori Shimanouchi ,Nachi Kitaura, RyoOnishi, Hiroshi Umakoshi, Ryoichi Kuboi
    • Journal Title

      AIChE J.

      Volume: 57 Pages: 3625-32

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Formatin of Spherulitic Amyloid βAggregate by Anionic Liposomes2012

    • Author(s)
      Toshinori Shimanouchi,NaoyaShimauhi,Ryo Ohnishi, Nachi Kitaura, Hiroshi Umakoshi, Ryoichi Kuboi
    • Journal Title

      Biophys. Biochem. Res. Comn

      Volume: 426 Pages: 165-71

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] リポソーム固定化技術によるメンブレノミクス研究2012

    • Author(s)
      島内寿徳
    • Journal Title

      生物物理

      Volume: 52 Pages: 154-5

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Membranome を基盤とするBio-Inspired膜へのアプローチ2012

    • Author(s)
      馬越大,島内寿徳,菅恵嗣
    • Journal Title

      膜

      Volume: 37 Pages: 264-9

    • NAID

      10031131135

    • Related Report
      2012 Final Research Report
  • [Journal Article] 人工細胞膜上におけるアミロイド形成2012

    • Author(s)
      島内寿徳,北浦奈知,馬越大,久保井亮一
    • Journal Title

      表面科学

      Volume: 33(1) Pages: 40-6

    • NAID

      10030443044

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Secondary nucleation of amyloid fibrils on liposome membranes2012

    • Author(s)
      T.Shimanouchi, N.Kitaura, R.Onishi, H.Umakoshi, R.Kuboi
    • Journal Title

      AlChE Journal

      Volume: (In press) Issue: 12 Pages: 3625-3632

    • DOI

      10.1002/aic.13772

    • Related Report
      2012 Annual Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Formatin of Spherulitic Amyloid βAggregate by Anionic Liposomes2012

    • Author(s)
      Toshinori Shimanouchi, Naoya Shimauchi, Ryo Ohnishi, Nachi Kitaura, Hiroshi Umakoshi, Ryoichi Kuboi
    • Journal Title

      Biophys. Biochem. Res. Comn.

      Volume: 426 Issue: 2 Pages: 165-171

    • DOI

      10.1016/j.bbrc.2012.07.107

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 人工細胞膜上におけるアミロイド形成2012

    • Author(s)
      島内寿徳,北浦奈知,馬越大,久保井亮一
    • Journal Title

      表面科学

      Volume: 33 Pages: 40-46

    • NAID

      10030443044

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Membranomeを基盤とするBio-Inspired 膜へのアプローチ2012

    • Author(s)
      馬越大,島内寿徳,菅恵嗣
    • Journal Title

      膜

      Volume: 37 Pages: 264-269

    • NAID

      10031131135

    • Related Report
      2012 Annual Research Report
  • [Journal Article] Effect of Copper (II) Ion against Elongation Behavior of Amyloid β Fibrils on Liposome Membranes2012

    • Author(s)
      Toshinori Shimanouchi et al.
    • Journal Title

      Crystal Research & Technology

      Volume: 47 Pages: 101-108

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Copper-Mediated Growth of Amyloid βFibrils in the presence of Oxidized and Negatively Charged Liposomes2011

    • Author(s)
      Toshinori Shimanouchi, Ryo Ohnishi, Nachi Kitaura, Ryoichi Kuboi, Hiroshi Umakoshi
    • Journal Title

      J. Biosci. Bioeng.

      Volume: 112 Pages: 611-5

    • NAID

      110008897338

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Relationship between the mobility of phosphocholine headgroups of liposomes and the hydrophobicity at the membrane interface: A characterization with spectrophotometric measurements2011

    • Author(s)
      Toshinori Shimanouchi, Masashi Sasaki, Azusa Hiroiwa, Noriko Yoshimoto, Kazuya Miyagawa, Hiroshi Umakoshi, Ryo Kuboi
    • Journal Title

      Colloids and Surfaces B

      Volume: 88 Pages: 221-23

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Effect of copper (II) ion against elongation behavior ofamyloid βfibrils on liposome membranes2011

    • Author(s)
      Toshinori Shimanouchi, Ryo Onishi, Nachi Kitaura, hiroshi Umakoshi
    • Journal Title

      Cryst. Res. Technol.

      Volume: 47 Pages: 101-8

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] 生体膜晶析工学の創成に関する基礎工学的研究2011

    • Author(s)
      島内寿徳
    • Journal Title

      膜

      Volume: 36(5) Pages: 233-9

    • NAID

      10029888018

    • Related Report
      2012 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Copper-Mediated Growth of Amyloid β Fibrils in the presence of Oxidized and Negatively Charged Liposomes2011

    • Author(s)
      T.Shimanouchi, R.Ohnishi, N.Kitaura, R.Kuboi, H.Umakoshi
    • Journal Title

      Journal of Bioscience and Bioengineering

      Volume: 112 Issue: 6 Pages: 611-615

    • DOI

      10.1016/j.jbiosc.2011.08.015

    • NAID

      110008897338

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Relationship between the mobility of phosphocholine headgroups of liposomes and the hydrophobicity at the membrane interface: A characterization with spectrophotometric measurements2011

    • Author(s)
      Toshinori Shimanouchi et al.
    • Journal Title

      Colloids and Surfaces B

      Volume: 88 Issue: 1 Pages: 221-230

    • DOI

      10.1016/j.colsurfb.2011.06.036

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] 人工細胞膜上におけるアミロイド形成2011

    • Author(s)
      島内寿徳 ら
    • Journal Title

      表面科学

      Volume: 33 Pages: 40-46

    • NAID

      10030443044

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] 異相界面におけるタンパク質の秩序構造制御~アミロイド形成を例に2013

    • Author(s)
      島内寿徳
    • Organizer
      第2回化学生命工学専攻談話会
    • Place of Presentation
      東京大学
    • Year and Date
      2013-02-02
    • Related Report
      2012 Final Research Report
  • [Presentation] 不均一膜組成を有する巨大ベシクルを用いたアミロイド形成現象の直接観察2013

    • Author(s)
      島内寿徳
    • Organizer
      化学工学会年会
    • Place of Presentation
      大阪大学(豊中キャンパス)
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Presentation] 異相界面におけるタンパク質の秩序構造制御 ~アミロイド形成を例に2013

    • Author(s)
      島内 寿徳
    • Organizer
      第2回化学生命工学専攻談話会
    • Place of Presentation
      東京大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] リポソーム膜によるアミロイドの微視的構造への影響2012

    • Author(s)
      島内 寿徳
    • Organizer
      膜シンポジウム
    • Place of Presentation
      神戸大学
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Presentation] 脂質分子からなるダイナミックな界面へのタンパク質の分配特性2012

    • Author(s)
      島内寿徳
    • Organizer
      溶媒抽出学会
    • Place of Presentation
      石川県文化会館
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Presentation] リポソームによるアミロイドの微視的構造の評価と制御2012

    • Author(s)
      島内寿徳,久保井亮一
    • Organizer
      化学工学会秋季大会
    • Place of Presentation
      東北大学(川内キャ ンパス)
    • Related Report
      2012 Final Research Report
  • [Presentation] アミロイド形成に及ぼすリポソーム/銅(II)の影響2012

    • Author(s)
      島内 寿徳
    • Organizer
      分離技術会年会
    • Place of Presentation
      関西大学
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Presentation] リポソーム膜により誘導されるAβ/Cuアミロイドのアスコルビン酸酸化酵素様活性2012

    • Author(s)
      島内寿徳
    • Organizer
      膜学会年会
    • Place of Presentation
      早稲田大学
    • Related Report
      2012 Final Research Report
  • [Presentation] リポソームによるアミロイドの微視的構造の評価と制御2012

    • Author(s)
      島内 寿徳,北浦 奈知,嶋内 直哉,馬越 大,久保井 亮一
    • Organizer
      化学工学会秋季大会
    • Place of Presentation
      東北大学(川内キャンパス)
    • Related Report
      2012 Annual Research Report
  • [Presentation] リポソーム膜により誘導されるA/Cuアミロイドのアスコルビン酸酸化酵素様活性2012

    • Author(s)
      島内 寿徳,大西 諒,北浦 奈知,馬越 大
    • Organizer
      膜学会年会
    • Place of Presentation
      早稲田大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] 生体膜晶析工学:脂質膜上におけるタンパク質のアミロイド形成2011

    • Author(s)
      島内寿徳
    • Organizer
      蛋白研セミナー
    • Place of Presentation
      蛋白研セミナーホール(大阪府)
    • Year and Date
      2011-04-28
    • Related Report
      2012 Final Research Report
  • [Presentation] 生体膜晶析工学の創成に関する基礎工学2011

    • Author(s)
      島内寿徳
    • Organizer
      日本膜学会年会
    • Place of Presentation
      産技総研 臨海副都心センター(東京都)
    • Related Report
      2012 Final Research Report
  • [Presentation] 生体膜を用いるタンパク質の晶析操作~生体膜晶析~2011

    • Author(s)
      島内寿徳
    • Organizer
      分離技術会年会
    • Place of Presentation
      明治大学生田キャンパス(神奈川県)
    • Related Report
      2012 Final Research Report
  • [Presentation] Amyloid fibril formation on model biomembranes2011

    • Author(s)
      T. Shimanouchi et al
    • Organizer
      ICSST11
    • Place of Presentation
      済州島,韓国
    • Related Report
      2012 Final Research Report
  • [Presentation] 生体膜晶析工学:脂質膜上におけるタンパク質のアミロイド形成2011

    • Author(s)
      島内 寿徳
    • Organizer
      蛋白研セミナー(招待講演)
    • Place of Presentation
      蛋白研セミナーホール(大阪府)
    • Related Report
      2011 Research-status Report
  • [Presentation] 生体膜晶析工学の創成に関する基礎工学(日本膜学会研究奨励賞受賞講演)2011

    • Author(s)
      島内 寿徳
    • Organizer
      日本膜学会年会(招待講演)
    • Place of Presentation
      産技総研 臨海副都心センター(東京都)
    • Related Report
      2011 Research-status Report
  • [Presentation] 生体膜を用いるタンパク質の晶析操作~生体膜晶析~2011

    • Author(s)
      島内 寿徳 ら
    • Organizer
      分離技術会年会(招待講演)
    • Place of Presentation
      明治大学生田キャンパス(神奈川県)
    • Related Report
      2011 Research-status Report
  • [Presentation] Amyloid fibril formation on model biomembranes2011

    • Author(s)
      Toshinori Shimanouchi et al.
    • Organizer
      International Conference on Separation Science & Technology 2011 (ICSST2011)
    • Place of Presentation
      済州島,韓国
    • Related Report
      2011 Research-status Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi