| Project/Area Number |
23656527
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Osaka Prefecture University (2012-2013)
Osaka University (2011) |
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Principal Investigator |
OKAZAWA Atsushi 大阪府立大学, 生命環境科学研究科(系), 准教授 (10294042)
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| Co-Investigator(Kenkyū-buntansha) |
OHTSUKI Takashi 山梨大学, 医学工学総合研究部, 准教授 (70313781)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 寄生植物 / 糖質加水分解酵素 / マンノシダーゼ / バイオマス変換 / 細胞壁多糖 / β-マンノシダーゼ / 細胞壁分解 / イソプレン |
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| Research Abstract |
Enzymes which can degrade cell-wall polysaccharides of plants are useful for effective conversion of plant biomass. In this project, we focused on a parasitic plant, Orobanche minor, which can degrade the cell-wall of roots of their host plants. From RNAseq data, a beta-glucosidase homologous gene was obtained. The enzyme expressed in E. coli showed beta-mannosidase activity when cell-wall polysaccharides from O. minor seeds were used as a substrate. This is the first report that indicates beta-mannosidase from plants showed activity toward plant cell-wall components.
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