Direct conversion of plant biomass to isoprene by microorganisms expressing cell-wall degrading enzymes from parasitic plants

• Project/Area Number: 23656527
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Biofunction/Bioprocess
• Research Institution: Osaka Prefecture University (2012-2013); Osaka University (2011)
• Principal Investigator: OKAZAWA Atsushi 大阪府立大学, 生命環境科学研究科(系), 准教授 (10294042)
• Co-Investigator(Kenkyū-buntansha): OHTSUKI Takashi 山梨大学, 医学工学総合研究部, 准教授 (70313781)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 寄生植物 / 糖質加水分解酵素 / マンノシダーゼ / バイオマス変換 / 細胞壁多糖 / β-マンノシダーゼ / 細胞壁分解 / イソプレン

Research Abstract

Enzymes which can degrade cell-wall polysaccharides of plants are useful for effective conversion of plant biomass. In this project, we focused on a parasitic plant, Orobanche minor, which can degrade the cell-wall of roots of their host plants. From RNAseq data, a beta-glucosidase homologous gene was obtained. The enzyme expressed in E. coli showed beta-mannosidase activity when cell-wall polysaccharides from O. minor seeds were used as a substrate. This is the first report that indicates beta-mannosidase from plants showed activity toward plant cell-wall components.

Research Products

• [Presentation] ヤセウツボ発芽種子で高発現する糖加水分解酵素の機能解析 (2014)
  • Author(s): 東久保諒, ギリモグレゴリー, 若林孝俊, 水谷正治, 杉本幸裕, 關光, 村中俊哉, 岡澤敦司
  • Organizer: 日本農芸化学会2014年度大会
  • Place of Presentation: 東京
• [Presentation] 根寄生雑草ヤセウツボの発芽時に発現するβ-マンノシダーゼの役割 (2014)
  • Author(s): 東久保諒，Gregory Guirimand，若林孝俊，水谷正治，杉本幸裕，關光，村中俊哉，岡澤敦司
  • Organizer: 日本農薬学会第 39 回大会
  • Place of Presentation: 京都大学農学部総合館
• [Presentation] ヤセウツボ発芽種子で発現する糖加水分解酵素の機能解析 (2014)
  • Author(s): 東久保諒，Gregory Guirimand，若林孝俊，水谷正治，杉本幸裕，關光，村中俊哉，岡澤敦司
  • Organizer: 日本農芸化学会 2014 年度大会
  • Place of Presentation: 明治大学生田キャンパス
• [Presentation] Functional characterization of β-mannosidase involved in the early germination process of Orobanche minor (2013)
  • Author(s): Guirimand, G., Higashikubo, R., Wakabayashi, T., Yasumoto, S., Mizutani, M., Sugimoto, Y., Seki, H., Muranaka, T., Okazawa, A
  • Organizer: 12th World Congress on Parasitic Plants
  • Place of Presentation: Sheffield, UK
• [Presentation] Functional characterization of β-mannosidase involved in the early germination process of the root-parasitic weed Orobanche minor (2013)
  • Author(s): Guirimand, G., Higashikubo, R., Wakabayashi, T., Yasumoto, S., Mizutani, M., Sugimoto, Y., Seki, H., Muranaka, T., Okazawa, A
  • Organizer: 第54回日本植物生理学会年会
  • Place of Presentation: 岡山
• [Presentation] Functional characterization of a beta-mannnosidase involved in the early germination process of Orobanche minor (2013)
  • Author(s): Guirimand, G., Higashikubo, R., Wakabayashi, T., Yasumoto, S., Mizutani, M., Sugimoto, Y., Seki, H., Muranaka, T., Okazawa, A.
  • Organizer: 12th World Congress on Parasitic Plants
  • Place of Presentation: Sheffield, UK
• [Presentation] Functional characterization of a β-mannosidase involved in the early germination process of the root-parasitic weed Orobanche minor (2013)
  • Author(s): Gregory Guirimand, Ryo Higashikubo, Takatoshi Wakabayashi, Shuhei Yasumoto, Masaharu Mizutani, Yukihiro Sugimoto, Hikaru Seki, Toshiya Muranaka, Atsushi Okazawa
  • Organizer: 第 54 回日本植物生理学会年会
  • Place of Presentation: 岡山大学 津島キャンパス
• [Presentation] ヤセウツボ発芽種子で発現する糖加水分解酵素の機能解析 (2013)
  • Author(s): 東久保諒，Gregory Guirimand，若林孝俊，水谷正治，杉本幸裕，關 光，村中俊哉，岡澤敦司
  • Organizer: 日本農芸化学会 2013 年度（平成 25 年度）大会
  • Place of Presentation: 東北大学 川内北キャンパス