| Project/Area Number |
23656529
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Hiroshima University |
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Principal Investigator |
KURODA Akio 広島大学, 大学院・先端物質科学研究科, 教授 (50205241)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
|
| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | ルシフェラーゼ / 迅速計測 / 微生物 / ATP / ATP増幅反応 |
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| Research Abstract |
To rapidly measure the number of microorganisms in solution, we attempted to use a capillary array, a circular glass plate with fine pore regularly arranged in two-dimensional array. After applying a given amount of sufficiently diluted solution to the capillary array containing both ATP-extracting and bioluminescence reagents, the number of microorganisms can be easily estimated from the proportion of luminescent pores. In this research, we immobilized luciferase on micro-beads (approximately 2.8 μm in diameter) that could serve as a model of microorganisms in solution. Using the micro-beads, we determined the concentration of ATP needed to generate detectable level of luminescence. We concluded that the rapid measurement of microorganisms would require further improvement of ATP amplification technology and the use of high luminescence luciferase.
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